scholarly journals Recent Progress on Biodegradable Tissue Engineering Scaffolds Prepared by Thermally-Induced Phase Separation (TIPS)

2021 ◽  
Vol 22 (7) ◽  
pp. 3504
Author(s):  
Reza Zeinali ◽  
Luis J. del Valle ◽  
Joan Torras ◽  
Jordi Puiggalí
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Material Selection ◽  
Three Dimensional ◽  
Target Cells ◽  
Porous Scaffolds ◽  
Thermally Induced Phase Separation ◽  
Tissue Engineering Scaffolds ◽  
Thermally Induced ◽  
Biodegradable Scaffolds

Porous biodegradable scaffolds provide a physical substrate for cells allowing them to attach, proliferate and guide the formation of new tissues. A variety of techniques have been developed to fabricate tissue engineering (TE) scaffolds, among them the most relevant is the thermally-induced phase separation (TIPS). This technique has been widely used in recent years to fabricate three-dimensional (3D) TE scaffolds. Low production cost, simple experimental procedure and easy processability together with the capability to produce highly porous scaffolds with controllable architecture justify the popularity of TIPS. This paper provides a general overview of the TIPS methodology applied for the preparation of 3D porous TE scaffolds. The recent advances in the fabrication of porous scaffolds through this technique, in terms of technology and material selection, have been reviewed. In addition, how properties can be effectively modified to serve as ideal substrates for specific target cells has been specifically addressed. Additionally, examples are offered with respect to changes of TIPS procedure parameters, the combination of TIPS with other techniques and innovations in polymer or filler selection.

Thermal Processing of Tissue Engineering Scaffolds

2010 ◽  
Vol 133 (3) ◽  
Author(s):  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Thermal Processing ◽  
Three Dimensional ◽  
Processing Parameters ◽  
High Pressure Processing ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Thermally Induced ◽  
Advantages And Disadvantages ◽  
Particulate Leaching

Tissue engineering requires complex three-dimensional scaffolds that mimic natural extracellular matrix function. A wide variety of techniques have been developed to create both fibrous and porous scaffolds out of polymers, ceramics, metals, and composite materials. Existing techniques include fiber bonding, electrospinning, emulsion freeze drying, solvent casting/particulate leaching, gas foaming/particulate leaching, high pressure processing, and thermally induced phase separation. Critical scaffold properties, including pore size, porosity, pore interconnectivity, and mechanical integrity, are determined by thermal processing parameters in many of these techniques. In this review, each tissue engineering scaffold preparation method is discussed, including recent advancements as well as advantages and disadvantages of the technique, with a particular emphasis placed on thermal parameters. Improvements on these existing techniques, as well as new thermal processing methods for tissue engineering scaffolds, will be needed to provide tissue engineers with finer control over tissue and organ development.

scholarly journals Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

RSC Advances ◽  
2015 ◽  
Vol 5 (27) ◽  
pp. 21301-21309 ◽  
Author(s):  
Xifeng Liu ◽  
A. Lee Miller II ◽  
Brian E. Waletzki ◽  
Michael J. Yaszemski ◽  
Lichun Lu
Keyword(s):  
Lactic Acid ◽  
Phase Separation ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Porous Structures ◽  
Polymer Scaffolds ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced ◽  
Biodegradable Poly ◽  
Poly Propylene

Three-dimensional polymer scaffolds with interconnected porous structures were fabricated by thermally induced phase separation of novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid).

Preparation of Three-Dimensional Scaffolds from Degradable Poly(ether)esterurethane by Thermally-Induced Phase Separation

2011 ◽  
Vol 309-310 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Karola Luetzow ◽  
Thomas Weigel ◽  
Michael Schossig ◽  
Karl Kratz ◽  
Andreas Lendlein
Keyword(s):  
Phase Separation ◽  
Three Dimensional ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

scholarly journals Water Uptake in PHBV/Wollastonite Scaffolds: A Kinetics Study

2019 ◽  
Vol 3 (3) ◽  
pp. 74 ◽  
Author(s):  
Ribas ◽  
Montanheiro ◽  
Montagna ◽  
Prado ◽  
Campos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Cell Growth ◽  
Cell Viability ◽  
Water Uptake ◽  
Kinetic Mechanism ◽  
Kinetics Study ◽  
Biological Applications ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a widely studied polymer and it has been found that porous PHBV materials are suitable for substrates for cell cultures. A crucial factor for scaffolds designed for tissue engineering is the water uptake. This property influences the transport of water and nutrients into the scaffold, which promotes cell growth. PHBV has significant hydrophobicity, which can harm the production of cells. Thus, the addition of α-wollastonite (WOL) can modify the PHBV scaffold’s water uptake. To our knowledge, a kinetics study of water uptake of α-wollastonite phase powder and the PHBV matrix has not been reported. In this work, PHBV and WOL, (PHBV/WOL) films were produced with 0, 5, 10, and 20 wt % of WOL. Films were characterized, and the best concentrations were chosen to produce PHBV/WOL scaffolds. The addition of WOL in concentrations up to 10 wt % increased the cell viability of the films. MTT analysis showed that PHBV/5%WOL and PHBV/10%WOL obtained cell viability of 80% and 98%, respectively. Therefore, scaffolds with 0, 5 and 10 wt % of WOL were fabricated by thermally induced phase separation (TIPS). Scaffolds were characterized with respect to morphology and water uptake in assay for 65 days. The scaffold with 10 wt % of WOL absorbed 44.1% more water than neat PHBV scaffold, and also presented a different kinetic mechanism when compared to other samples. Accordingly, PHBV/WOL scaffolds were shown to be potential candidates for biological applications.

Comparison of Supermacroporous Polyester Matrices Fabricated by Thermally Induced Phase Separation and 3D Printing Techniques

2019 ◽  
Vol 822 ◽  
pp. 277-283
Author(s):  
Mariia Stepanova ◽  
Aleksei Eremin ◽  
Ilia Averianov ◽  
Iosif Gofman ◽  
Antonina Lavrentieva ◽  
...  
Keyword(s):  
Phase Separation ◽  
3D Printing ◽  
Three Dimensional ◽  
Uniaxial Compression Test ◽  
Thermally Induced Phase Separation ◽  
Cell Penetration ◽  
Thermally Induced ◽  
Printing Technique ◽  
3D Printing Technique ◽  
Printing Techniques

Supermacroporous three-dimensional matrices based on poly-D,L-lactide or polycaprolactone were fabricated by thermally induced phase separation method and 3D printing technique. The morphology and mechanical properties of the resulting matrices were studied with the use of optical and scanning electron microscopy and the uniaxial compression test, respectively. All matrices were characterized with supermacroporous structure suitable for cell penetration. A significant increase in Young's modulus and tensile strength was established for both polymer matrices prepared by 3D printing technique.

APPLICATIONS OF CALCIUM PHOSPHATE NANOPARTICLES IN POROUS HARD TISSUE ENGINEERING SCAFFOLDS

NANO ◽  
2012 ◽  
Vol 07 (04) ◽  
pp. 1230004 ◽  
Author(s):  
ZHE WANG ◽  
ZHURONG TANG ◽  
FANGZHU QING ◽  
YOULIANG HONG ◽  
XINGDONG ZHANG
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Important Approach ◽  
Calcium Phosphate Nanoparticles ◽  
Hard Tissue Engineering

To repair bone defects, an important approach is to fabricate tissue engineering scaffolds as substitutions to replace auto-/allologous bones. Currently, processing a biomaterial into three-dimensional porous scaffolds and incorporating the calcium phosphate (Ca–P) nanoparticles into scaffolds profile two main characteristics of bone tissue engineering scaffolds. Based on this fact, in this paper we describe the design principles of the Ca–P nanoparticle-based and porous bone tissue engineering scaffolds. Then we summarize a variety of the Ca–P nanoparticle-based scaffolds, including discussion of the integration of the Ca–P nanoparticles with ceramics and polymers, followed by introduction of safety of the Ca–P nanoparticles in scaffolds.

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