Development of new graphene oxide incorporated tricomponent scaffolds with polysaccharides and hydroxyapatite and study of their osteoconductivity on MG-63 cell line for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (51) ◽  
pp. 41135-41143 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Gellan Gum ◽  
First Time

GO–alginate–HAP, GO–amylopectin–HAP and GO–gellan gum–HAP were prepared and characterized and their osteoconductivity were checked for the first time.

Development of functionalized multi-walled carbon nanotube-based polysaccharide–hydroxyapatite scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (85) ◽  
pp. 82385-82393 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran ◽  
M. Jeevan Kumar Reddy ◽  
Sung Hun Ryu ◽  
A. M. Shanmugharaj
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Alp Activity ◽  
Multi Walled Carbon Nanotube ◽  
First Time

fMWCNT–amylopectin–HAP and fMWCNT–gellan gum–HAP were prepared and characterized and their in vitro cell proliferation and ALP activity were checked for the first time.

Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

2018 ◽  
Vol 32 (10) ◽  
pp. 1392-1405 ◽  
Author(s):  
Ali Deniz Dalgic ◽  
Ammar Z. Alshemary ◽  
Ayşen Tezcaner ◽  
Dilek Keskin ◽  
Zafer Evis
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Protein Adsorption ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Osteosarcoma Cell ◽  
In Vitro Biocompatibility ◽  
Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

scholarly journals Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Jiaqi Zhu ◽  
Zhiping Qi ◽  
Changjun Zheng ◽  
Pan Xue ◽  
Chuan Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Graphene Oxide ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Cellular Response ◽  
Hybrid Fiber ◽  
Fiber Matrices

Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

scholarly journals Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure

RSC Advances ◽  
2020 ◽  
Vol 10 (17) ◽  
pp. 10118-10128 ◽  
Author(s):  
Lu Wang ◽  
Min Fang ◽  
Yijing Xia ◽  
Jiaxin Hou ◽  
Xiaoru Nan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Biological Properties ◽  
Hybrid Scaffold

A novel SF/nHAp/GO hybrid scaffold with oriented channel-like structure in bone tissue engineering.

Self-mineralizing Ca-enriched methacrylated gellan gum beads for bone tissue engineering

2019 ◽  
Vol 93 ◽  
pp. 74-85 ◽  
Author(s):  
Sílvia Vieira ◽  
Alain da Silva Morais ◽  
Elina Garet ◽  
Joana Silva-Correia ◽  
Rui L. Reis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum

Nanoparticulate bioactive-glass-reinforced gellan-gum hydrogels for bone-tissue engineering

2014 ◽  
Vol 43 ◽  
pp. 27-36 ◽  
Author(s):  
Ana Gantar ◽  
Lucilia P. da Silva ◽  
Joaquim M. Oliveira ◽  
Alexandra P. Marques ◽  
Vitor M. Correlo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum

Fabrication of Porous α-TCP/Gellan Gum Scaffold for Bone Tissue Engineering

2016 ◽  
Vol 16 (3) ◽  
pp. 3077-3083 ◽  
Author(s):  
Jian Wen ◽  
Ill Yong Kim ◽  
Koichi Kikuta ◽  
Chikara Ohtsuki
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum

Differentiation of osteoclast precursors on gellan gum-based spongy-like hydrogels for bone tissue engineering

2018 ◽  
Vol 13 (3) ◽  
pp. 035012 ◽  
Author(s):  
F Raquel Maia ◽  
David S Musson ◽  
Dorit Naot ◽  
Lucilia P da Silva ◽  
Ana R Bastos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Osteoclast Precursors
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