Optimization and hydrolysis of cellulose under subcritical water treatment for the production of total reducing sugars

RSC Advances ◽  
2015 ◽  
Vol 5 (125) ◽  
pp. 103265-103275 ◽  
Author(s):  
Mood Mohan ◽  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Tamal Banerjee ◽  
Vaibhav V. Goud ◽  
...  
Keyword(s):  
Organic Matter ◽  
Water Treatment ◽  
Process Parameters ◽  
Reducing Sugars ◽  
Subcritical Water ◽  
Reaction Medium ◽  
Hydrolysis Of Cellulose ◽  
Total Reducing Sugars ◽  
Rsm Model ◽  
Hydrolysis Of

Subcritical water (SCW) treatment has gained enormous attention as an environmentally friendly technique for organic matter and an attractive reaction medium for a variety of applications. In the current work the process parameters were optimized by RSM model.

Cellulose Hydrolysis by Acidic Ionic Liquids Enhanced with Microwave Heating

2018 ◽  
Vol 1145 ◽  
pp. 75-79 ◽  
Author(s):  
Hai Yun Ma ◽  
Zhi Ping Zhao ◽  
Peng Lu
Keyword(s):  
Ionic Liquids ◽  
Microwave Heating ◽  
Reducing Sugars ◽  
Cellulose Hydrolysis ◽  
Degree Of Polymerization ◽  
Deionized Water ◽  
Acidic Ionic Liquids ◽  
Hydrolysis Of Cellulose ◽  
Total Reducing Sugars ◽  
Hydrolysis Of

The hydrolysis of cellulose into platform compounds and chemicals fuels has gained much attention to relieve the global energy crisis and environmental pollution. The filter paper (FP) cellulose with average degree of polymerization (DP) of 1000-1300 was dissolved in 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) firstly. And then acidic ionic liquids (ILs), ([(CH2)3SO3HVIm]HSO4) as the catalyst was applied to hydrolyze the FP cellulose by microwave heating. Compared with the oil bath heating method, microwave heating could effectively increase the total reducing sugars (TRS) yield about 10.7%. When the ratio of ILs catalyst to FP (w/w) was 0.167, and the ratio of deionized water to FP (w/w) was 0.833, the TRS yield was up to 60.8% within 20 min at 100°C.

Enhanced Hydrolysis of Cellulose to Reducing Sugars on Kaolinte Clay Activated by Mineral Acid

2021 ◽  
Author(s):  
Yuxiao Dong ◽  
Dongshen Tong ◽  
Laibin Ren ◽  
Xingtao Chen ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Mineral Acid ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Hydrolysis of bamboo biomass by subcritical water treatment

2015 ◽  
Vol 191 ◽  
pp. 244-252 ◽  
Author(s):  
Mood Mohan ◽  
Tamal Banerjee ◽  
Vaibhav V. Goud
Keyword(s):  
Water Treatment ◽  
Subcritical Water ◽  
Hydrolysis Of

Bioconversion of De-Oiled Rice Bran (DORB) Hemicellulosic Hydrolysate into Ethanol by Pichia stipitis NCM3499 under Optimized Conditions

2009 ◽  
Vol 5 (1) ◽  
Author(s):  
Anuj Kumar Chandel ◽  
Mangamoori Lakshmi Narasu ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
Linga Venkateswar Rao
Keyword(s):  
Ethanol Production ◽  
Rice Bran ◽  
Process Parameters ◽  
Reducing Sugars ◽  
Carbon Sources ◽  
Pichia Stipitis ◽  
Medium Temperature ◽  
Carbon Substrates ◽  
Total Reducing Sugars ◽  
Optimized Conditions

In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.

Pyridinium based ionic liquid: A pretreatment solvent and reaction medium for catalytic conversion of cellulose to total reducing sugars (TRS)

2018 ◽  
Vol 272 ◽  
pp. 330-336 ◽  
Author(s):  
Saliha Saher ◽  
Haris Saleem ◽  
Azmat Mehmood Asim ◽  
Maliha Uroos ◽  
Nawshad Muhammad
Keyword(s):  
Ionic Liquid ◽  
Reducing Sugars ◽  
Reaction Medium ◽  
Catalytic Conversion ◽  
Total Reducing Sugars

scholarly journals A Thermophilic GH5 Endoglucanase from Aspergillus fumigatus and Its Synergistic Hydrolysis of Mannan-Containing Polysaccharides

Catalysts ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 862
Author(s):  
Xinxi Gu ◽  
Haiqiang Lu ◽  
Lijuan Zhang ◽  
Xiangchen Meng
Keyword(s):  
Aspergillus Fumigatus ◽  
Reducing Sugars ◽  
Hydrolytic Activity ◽  
The Novel ◽  
Carbohydrate Active Enzymes ◽  
Polysaccharide Lyase ◽  
Optimal Activity ◽  
Total Reducing Sugars ◽  
Encoding Genes ◽  
Hydrolysis Of

In this study, we isolated and identified a thermophilic strain of Aspergillus fumigatus from the “Daqu” samples. Transcriptomic analysis of A. fumigatus identified 239 carbohydrate-active enzymes (CAZy)-encoding genes, including 167 glycoside hydrolase (GH)-encoding genes, 58 glycosyltransferase (GT)-encoding genes, 2 polysaccharide lyase (PLs)-encoding genes and 12 carbohydrate esterase (CEs)-encoding genes, which indicates that the strain has a strong potential for application for enzyme production. Furthermore, we also identified a novel endoglucanase gene (AfCel5A), which was expressed in Pichia pastoris and characterized. The novel endoglucanase AfCel5A exhibited the highest hydrolytic activity against CMC-Na and the optimal activity at 80 °C and pH 4.0 and also showed good stability at pH 3.0–11.0 and below 70 °C. The Km and Vmax values of AfCel5 were 0.16 ± 0.05 mg·mL−1 and 7.23 ± 0.33 mol mg−1·min−1, respectively, using CMC-Na as a substrate. Further, the endoglucanase exhibited a high tolerance toward NaCl as well as glucose. In addition, the finding that the endoglucanase AfCel5A in combination with β-mannanse (ManBK) clearly increased the release of total reducing sugars of glucomannan by up to 74% is significant.

scholarly journals Effect of Combinative Pretreatments on Cellulose-to-Glucose Conversion of Empty Palm Fruit Bunch (EFB)

2014 ◽  
Vol 7 ◽  
pp. 81-85
Author(s):  
Yakindra Prasad Timilsena ◽  
Nicolas Brosse
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugars ◽  
Dilute Acid ◽  
Palm Fruit ◽  
Step Counter ◽  
Lignin Removal ◽  
One Step ◽  
Total Reducing Sugars ◽  
Hydrolysis Of ◽  
Sugar Conversion

Various methods of pretreatments were investigated to their effect on cellulose to glucose conversion efficiency on enzymatic hydrolysis of a tropical agro-industrial waste residue (empty palm fruit bunch, EFB). Four different kinds of combinative pretreatments (autohydrolysis with and without naphthol, dilute acid prehydrolysis, soda prehydrolysis and enzymatic prehydrolysis) were tested for delignification during the first and the second steps of pretreatment. Each prehydrolysis step was seconded by the organosolv delignification with the same conditions of pretreatment. It was observed that all the combinative methods were far more efficient in delignification and enzymatic hydrolysis ability as compared to its one step counter parts. The combinative pretreatment method involving dilute acid prehydrolysis followed by organosolv delignification revealed the best result with respect to lignin removal and enzymatic hydrolysis. The resultant pulp contained very low Klason lignin (~5%) with high sugar conversion ratio (64% total reducing sugars). DOI: http://dx.doi.org/10.3126/jfstn.v7i0.10613   J. Food Sci. Technol. Nepal, Vol. 7 (81-85), 2012  

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