Influence of photoactivated tetra sulphonatophenyl porphyrin and TiO2nanowhiskers on rheumatoid arthritis infected bone marrow stem cell proliferation in vitro and oxidative stress biomarkers in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (130) ◽  
pp. 107285-107292 ◽  
Author(s):  
Fawad Ur Rehman ◽  
Chunqiu Zhao ◽  
Changyu Wu ◽  
Hui Jiang ◽  
Matthias Selke ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress Biomarkers ◽  
Promoting Effect ◽  
Stress Biomarkers ◽  
Growth Promoting ◽  
Proliferation In Vitro ◽  
And Oxidative Stress ◽  
Marrow Stem Cell

Besides the lethal effects of photodynamic therapy on neoplasms, herein we report photoactivated TSPP–TiO2nanocomposites' growth promoting effect on rheumatoid arthritis BMS cells.

scholarly journals The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alireza Rezaeinezhad ◽  
Pegah Eslami ◽  
Hossein Mirmiranpour ◽  
Hamid Ghomi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Fluorescence Analysis ◽  
Atmospheric Plasma ◽  
Oxidative Stress Biomarkers ◽  
Cold Atmospheric Plasma ◽  
Stress Biomarkers

AbstractCold atmospheric plasma (CAP) is known as the versatile tool in different biological, and medical applications. In this study, we investigated the effect of cold plasma on diabetes via in vitro and in vivo assessments. We performed the in vitro assay to evaluate the impact of CAP on glycated glutathione peroxidase (GPx) through enzyme activity measurement as a function index and far- and near-UV circular dichroism (CD) and fluorescence analysis as structure indices. The result of in vitro assessment showed that the exposure of glycated GPx to plasma causes a considerable increase in enzyme activity up to 30%. Also, the evaluation of far- and near-UV CD and fluorescence analysis indicated a modification in the protein structure. According to obtained result from in vitro assessment, in vivo assay evaluated the effect of CAP on diabetic mice through analyzing of blood glucose level (BGL), advanced glycation end products (AGEs), antioxidant activity, oxidative stress biomarkers such as malondialdehyde (MDA), advanced oxidation protein products (AOPP), and oxidized low-density lipoprotein (oxLDL), and inflammation factors including tumor necrosis factor (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6). The result of in vivo experiment also showed a 20% increase in antioxidant activity. Also, the reduction in AGEs, oxidative stress biomarkers, and inflammatory cytokines concentrations was observed. The result of this study revealed that CAP could be useful in diabetes treatment and can be utilized as a complementary method for diabetes therapy.

In vivo and in vitro hair growth‐promoting effect of silver and iron nanoparticles synthesized via Blumea eriantha DC plant extract

2020 ◽  
Author(s):  
Rohankumar R. Chavan ◽  
Somnath D. Bhinge ◽  
Mangesh A. Bhutkar ◽  
Dheeraj S. Randive ◽  
Ganesh H. Wadkar ◽  
...  
Keyword(s):  
Plant Extract ◽  
Hair Growth ◽  
Iron Nanoparticles ◽  
Promoting Effect ◽  
Growth Promoting

scholarly journals Hair Growth Promoting Effect of Black Soybean Extract In Vitro and In Vivo

2011 ◽  
Vol 43 (6) ◽  
pp. 747-753 ◽  
Author(s):  
Hee-Young Jeon ◽  
Seung-Hun Kim ◽  
Chae-Wook Kim ◽  
Hyun-Jeong Shin ◽  
Dae-Bang Seo ◽  
...  
Keyword(s):  
Hair Growth ◽  
Promoting Effect ◽  
Black Soybean ◽  
Soybean Extract ◽  
Growth Promoting

scholarly journals Short-Term Bixin Supplementation of Healthy Subjects Decreases the Susceptibility of LDL to Cu2+-Induced Oxidation Ex Vivo

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Lisiane Conte ◽  
Sabrina Somacal ◽  
Sabrina Marafiga Nichelle ◽  
Cristine Rampelotto ◽  
Silvino Sasso Robalo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Biochemical Parameters ◽  
Ex Vivo ◽  
Ldl Oxidation ◽  
Short Term ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress

Lycopene-based medications and supplements have been developed to prevent atherosclerosis, primarily because of their ability to decrease low-density lipoprotein (LDL) oxidation. Bixin and norbixin are carotenoids found in the seeds of annatto (Bixa orellana) and are colorants widely used by the food industry. Some studies have already demonstrated that these compounds have antioxidant and antiatherogenic potential in vitro and in animal models, but there is no evidence supporting the effects of their long-term or short-term consumption by humans. The aim of this study was to evaluate the effects of short-term intake of annatto carotenoids on biochemical and oxidative stress biomarkers as well as on the susceptibility of LDL oxidation in healthy individuals, using lycopene as a positive control. The effect of daily supplementation (0.05 mg/kg of body weight (b.w.)) with bixin, norbixin, lycopene, or placebo for 7 days was evaluated in a randomized, controlled crossover study in 16 healthy volunteers (8 men and 8 women). The susceptibility of LDL to Cu2+-induced oxidation ex vivo, biochemical parameters, and oxidative stress biomarkers were evaluated. No treatment affected biochemical parameters or most oxidative stress biomarkers. However, bixin reduced the oxidation rate of the LDL lipid moiety (−275%, p<0.1) and nitric oxide metabolites (NOx) (−460%, p<0.1), compared to the placebo group. Moreover, we observed that the changes in these parameters were positively associated, supporting the hypothesis that bixin decreases the susceptibility of LDL to Cu2+-induced oxidation by decreasing NOx levels, probably by downregulating the inducible nitric oxide synthase.

scholarly journals Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

2020 ◽  
Vol 10 (10) ◽  
pp. 3602 ◽  
Author(s):  
Javier Hernández-Fernández ◽  
Ellie Anne López-Barrera ◽  
Leonardo Mariño-Ramírez ◽  
Pilar Rodríguez-Becerra ◽  
Andrés Pinzón-Velasco
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Loggerhead Turtle ◽  
In Vitro Models ◽  
Glutathione S Transferase ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Loggerhead Turtles ◽  
And Oxidative Stress

This study describes the use of erythrocytes (RBCs) of loggerhead turtles as in vitro models for evaluating their toxicity to methylmercury. Blood samples of loggerhead turtles that were born in the Colombian Caribbean were used. The LC50 of RBCs to methylmercury was determined at 96 h using methylmercury concentrations of 0.5–100 mg L−1. Next, the viability of the RBCs and the activity of the enzymes superoxide dismutase (SOD), glutathione S-transferase (GST), and lipid peroxidation by malondialdehyde (MDA) at 6 and 12 h of exposure to acute concentrations of 0, 1, and 5 mg L−1 were evaluated. The LC50 for loggerhead turtle RBCs was 8.32 mg L−1. The cell viability bioassay of RBCs exposed for 12 h only showed 100% cell viability. Increasing in vitro MeHg concentrations caused a corresponding increase in MDA concentration as well as decreases in the activities of SOD and GST. The RBCs represent an excellent model for ecotoxicological studies and SOD, GST, and MDA are biomarkers of environmental pollution and oxidative stress in loggerhead turtles. This was the first study conducted on loggerhead turtle where the response of RBCs to MeHg-induced oxidative stress is evaluated.

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