Development of a gradient micellar liquid chromatographic method eluting from micellar mode to high submicellar mode for the rapid separation of free amino acids

2017 ◽  
Vol 9 (11) ◽  
pp. 1762-1770 ◽  
Author(s):  
Jing Ke ◽  
Yuming Dong ◽  
Tian Luo ◽  
Yunfei Xie
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Free Amino Acids ◽  
Free Amino ◽  
Chromatographic Method ◽  
Rapid Separation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

A rapid surfactant-related gradient liquid chromatographic method progressing from a micellar liquid chromatographic mode to a submicellar liquid chromatographic mode has been developed for the quantitative analysis of free amino acids.

Chromatographic Separation of Free Amino Acids in Table Sirups

1971 ◽  
Vol 54 (1) ◽  
pp. 61-65
Author(s):  
Arthur Russell Johnson ◽  
Richard L Corliss ◽  
Enrique Fernandez-Flores
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Butyl Esters ◽  
Tlc Analysis

Abstract Qualitative chromatographic methods for the separation of free amino acids in table sirups are presented to aid in the development of chemical indices of composition which may be useful in establishing the identity of sirups and detecting their adulteration. Free amino acids in 2 table sirups were isolated on ion exchange columns and eluted with dilute ammonia. The concentrated amino acid mixture in the eluate was spotted directly on silica gel G plates for TLC analysis, or the amino acids were converted to their N-trifluoroacetyl n-butyl esters for GLC analysis. As many as 16 amino acids were qualitatively separated and identified and a potential for quantitative analysis was demonstrated.

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