Multi-color polymer pen lithography for oligonucleotide arrays

2016 ◽  
Vol 52 (83) ◽  
pp. 12310-12313 ◽  
Author(s):  
R. Kumar ◽  
S. Weigel ◽  
R. Meyer ◽  
C. M. Niemeyer ◽  
H. Fuchs ◽  
...  
Keyword(s):  
Single Cell ◽  
Oligonucleotide Arrays ◽  
Multiple Protein ◽  
Polymer Pen Lithography ◽  
Color Patterning

Multi-color patterning by polymer pen lithography (PPL) was used to fabricate covalently immobilized fluorophore and oligonucleotide arrays with up to five different components. These can easily be translated for presentation of multiple protein types to a single cell.

Detection of ≥1 Mb microdeletions and microduplications in a single cell using custom oligonucleotide arrays

2012 ◽  
Vol 32 (1) ◽  
pp. 10-20 ◽  
Author(s):  
Weimin Bi ◽  
Amy Breman ◽  
Chad A. Shaw ◽  
Pawel Stankiewicz ◽  
Tomasz Gambin ◽  
...  
Keyword(s):  
Single Cell ◽  
Oligonucleotide Arrays

scholarly journals Polymer Pen Lithography-Fabricated DNA Arrays for Highly Sensitive and Selective Detection of Unamplified Ganoderma Boninense DNA

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 561 ◽  
Author(s):  
Ekta Rani ◽  
Siti Mohshim ◽  
Muhammad Ahmad ◽  
Royston Goodacre ◽  
Shahrul Alang Ahmad ◽  
...  
Keyword(s):  
Soft Materials ◽  
Selective Detection ◽  
Dna Arrays ◽  
Ganoderma Boninense ◽  
Laboratory Equipment ◽  
Oligonucleotide Arrays ◽  
Scanning Probe Lithography ◽  
Target Dna ◽  
Polymer Pen Lithography ◽  
Increasing Demand

There is an increasing demand for lithography methods to enable the fabrication of diagnostic devices for the biomedical and agri-food sectors. In this regard, scanning probe lithography methods have emerged as a possible approach for this purpose, as they are not only convenient, robust and accessible, but also enable the deposition of “soft” materials such as complex organic molecules and biomolecules. In this report, the use of polymer pen lithography for the fabrication of DNA oligonucleotide arrays is described, together with the application of the arrays for the sensitive and selective detection of Ganoderma boninense, a fungal pathogen of the oil palm. When used in a sandwich assay format with DNA-conjugated gold nanoparticles, this system is able to generate a visually observable result in the presence of the target DNA. This assay is able to detect as little as 30 ng of Ganoderma-derived DNA without any pre-amplification and without the need for specialist laboratory equipment or training.

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

Unravelling Single Cell Genomics

2010 ◽  
Keyword(s):  
Single Cell ◽  
Single Cell Genomics

Use of the single cell gel electrophoresis assay (Comet assay) as a technique for monitoring low-temperature treated and irradiated muscle tissues

2000 ◽  
Vol 35 (6) ◽  
pp. 555-561 ◽  
Author(s):  
Jong-Heum Park ◽  
Chang-Kee Hyun ◽  
Seok-Kyu Jeong ◽  
Mi-Ae Yi ◽  
Seung-Taek Ji ◽  
...  
Keyword(s):  
Low Temperature ◽  
Comet Assay ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis ◽  
Muscle Tissues
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