Improved assessment of accuracy and performance indicators in paper-based ELISA

The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the “gold standard” involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.

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Point-of-Care Systems for Rapid DNA Quantification in Oncology

Tumori Journal ◽

10.1177/030089160809400214 ◽

2008 ◽

Vol 94 (2) ◽

pp. 216-225 ◽

Cited By ~ 5

Author(s):

Marco Bianchessi ◽

Sarah Burgarella ◽

Marco Cereda

Keyword(s):

Point Of Care ◽

Low Cost ◽

Semiconductor Industry ◽

Point Of Care Testing ◽

Lab On Chip ◽

Diagnostic Assays ◽

Care Systems ◽

Point Of Care Systems ◽

The Common ◽

On Chip

The development of new powerful applications and the improvement in fabrication techniques are promising an explosive growth in lab-on-chip use in the upcoming future. As the demand reaches significant levels, the semiconductor industry may enter in the field, bringing its capability to produce complex devices in large volumes, high quality and low cost. The lab-on-chip concept, when applied to medicine, leads to the point-of-care concept, where simple, compact and cheap instruments allow diagnostic assays to be performed quickly by untrained personnel directly at the patient's side. In this paper, some practical and economical considerations are made to support the advantages of point-of-care testing. A series of promising technologies developed by STMicroelectronics on lab-on-chips is also presented, mature enough to enter in the common medical practice. The possible use of these techniques for cancer research, diagnosis and treatment are illustrated together with the benefits offered by their implementation in point-of-care testing.

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Integrated point of care testing system based on low cost polymer biochips

10.1117/12.808160 ◽

2009 ◽

Author(s):

Albrecht Brandenburg ◽

Franziska Curdt ◽

Joerg Nestler ◽

Thomas Otto ◽

Kai Wunderlich ◽

...

Keyword(s):

Point Of Care ◽

Low Cost ◽

Testing System ◽

Point Of Care Testing

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Four Dissemination Pathways for a Social Media–Based Breastfeeding Campaign: Evaluation of the Impact on Key Performance Indicators (Preprint)

10.2196/preprints.14589 ◽

2019 ◽

Author(s):

Kassandra Harding ◽

Rafael Pérez-Escamilla ◽

Grace Carroll ◽

Richmond Aryeetey ◽

Opeyemi Lasisi

Keyword(s):

Social Media ◽

Performance Indicators ◽

Performance Indicator ◽

Low Cost ◽

Social Media Marketing ◽

Campaign Evaluation ◽

Indicator Data ◽

And Performance ◽

Top 40 ◽

The Impact

BACKGROUND Social media utilization is on the rise globally, and the potential of social media for health behavior campaigns is widely recognized. However, as the landscape of social media evolves, so do techniques used to optimize campaign dissemination. OBJECTIVE The primary aim of this study was to evaluate the impact of 4 material dissemination paths for a breastfeeding social media marketing campaign in Ghana on exposure and engagement with campaign material. METHODS Campaign materials (n=60) were posted to a Facebook and Twitter campaign page over 12 weeks (ie, baseline). The top 40 performing materials were randomized to 1 of 4 redissemination arms (control simply posted on each platform, key influencers, random influencers, and paid advertisements). Key performance indicator data (ie, exposure and engagement) were extracted from both Facebook and Twitter 2 days after the material was posted. A difference-in-difference model was used to examine the impact of the dissemination paths on performance. RESULTS At baseline, campaign materials received an average (SD) exposure of 1178 (670) on Facebook and 1071 (905) on Twitter (n=60). On Facebook, materials posted with paid advertisements had significantly higher exposure and engagement compared with the control arm (<italic>P</italic><.001), and performance of materials shared by either type of influencer did not differ significantly from the control arm. No differences in Twitter performance were detected across arms. CONCLUSIONS Paid advertisements are an effective mechanism to increase exposure and engagement of campaign posts on Facebook, which was achieved at a low cost.

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Low-Cost Microdevices for Point-of-Care Testing

Point-of-Care Diagnostics on a Chip - Biological and Medical Physics, Biomedical Engineering ◽

10.1007/978-3-642-29268-2_1 ◽

2012 ◽

pp. 3-21 ◽

Cited By ~ 26

Author(s):

Curtis D. Chin ◽

Sau Yin Chin ◽

Tassaneewan Laksanasopin ◽

Samuel K. Sia

Keyword(s):

Point Of Care ◽

Low Cost ◽

Point Of Care Testing

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Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Sensors ◽

10.3390/s20216073 ◽

2020 ◽

Vol 20 (21) ◽

pp. 6073

Author(s):

Chunyang Lu ◽

Jintao Han ◽

Xiaoyi Sun ◽

Gen Yang

Keyword(s):

Tumor Cells ◽

Circulating Tumor Cells ◽

Electrochemical Method ◽

Point Of Care ◽

Low Cost ◽

High Sensitivity ◽

Point Of Care Testing ◽

Testing Instrument ◽

The Past ◽

Main Target

Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

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