Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

2017 ◽  
Vol 46 (40) ◽  
pp. 13957-13965 ◽  
Author(s):  
Shuoren Du ◽  
Javier Hernández-Gil ◽  
Hao Dong ◽  
Xiaoyu Zheng ◽  
Guangming Lyu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Ph Sensitive ◽  
Upconversion Nanoparticles ◽  
Imaging Probe ◽  
Ph Imaging ◽  
Ratiometric Probe ◽  
Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

scholarly journals 3P180 Intracellular pH measurement of Salmonella with a pH-sensitive GFP, pHluorin, and a newly developed pH imaging system(Molecular motors,Poster Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S248
Author(s):  
Yusuke Morimoto ◽  
Yong-Suk Che ◽  
Tohru Minamino ◽  
Nobunori Kami-ike ◽  
Keiichi Namba
Keyword(s):  
Intracellular Ph ◽  
Molecular Motors ◽  
Imaging System ◽  
Ph Sensitive ◽  
Ph Measurement ◽  
Ph Imaging ◽  
Poster Presentations

A profluorescent ratiometric probe for intracellular pH imaging

Talanta ◽  
2015 ◽  
Vol 131 ◽  
pp. 666-671 ◽  
Author(s):  
Qingqing Zhang ◽  
Ming Zhou
Keyword(s):  
Intracellular Ph ◽  
Ph Imaging ◽  
Ratiometric Probe

A Fluorescence Ratiometric Probe for Imaging of Acidic pH in Living Cells

2013 ◽  
Vol 864-867 ◽  
pp. 83-87
Author(s):  
Ling Liang Long ◽  
Yan Jun Wu ◽  
Lin Wang
Keyword(s):  
Fluorescence Emission ◽  
Emission Spectra ◽  
Living Cells ◽  
Acidic Ph ◽  
X Ray Diffraction ◽  
Fluorescence Emission Spectra ◽  
H Nmr ◽  
Ph Imaging ◽  
Visible Effect ◽  
Ratiometric Probe

A new fluorescent probe 1, bearing a pyridine group as receptor for H+and a coumarin dye as fluorophore, was synthesized and characterized by1H NMR,13C NMR, ESI-Ms and single crystal X-ray diffraction analysis. The probe exhibited fluorescence ratiometric response to acidic pH. With decreasing of the pH from 8.32 to 2.49, the fluorescence emission spectra exhibited large red shift from 541 to 631 nm, with the emission ratios (I541/I631) changed dramatically from 25.9 to 0.08, and the pKavalue was calculated to be 5.45. Probe 1 exhibited high selectivity to pH, other interference species including metal ions and amino acid exerted no visible effect on probe 1 detecting pH. The intracellular pH imaging applications proved that the probe is suitable for monitoring acidic pH fluctuations in living cells.

scholarly journals Live-Cell Imaging and Measurement of Intracellular pH in Filamentous Fungi Using a Genetically Encoded Ratiometric Probe

2009 ◽  
Vol 8 (5) ◽  
pp. 703-712 ◽  
Author(s):  
Tanja Bagar ◽  
Kirsten Altenbach ◽  
Nick D. Read ◽  
Mojca Benčina
Keyword(s):  
Intracellular Ph ◽  
Chimeric Protein ◽  
Weak Acid ◽  
Cytoplasmic Ph ◽  
Ph Gradients ◽  
Ph Values ◽  
Ph Imaging ◽  
Carbonyl Cyanide ◽  
Ratio Imaging ◽  
Ratiometric Probe

ABSTRACT A novel, genetically encoded, ratiometric pH probe (RaVC) was constructed to image and measure intracellular pH in living hyphae of Aspergillus niger. RaVC is a chimeric protein based on the pH-sensitive probe pHluorin, which was partially codon optimized for expression in Aspergillus. Intracellular pH imaging and measurement was performed by simultaneous, dual-excitation confocal ratio imaging. The mean cytoplasmic pH measured was 7.4 to 7.7 based on calibrating RaVC in situ within nigericin-treated hyphae. Pronounced, longitudinal cytoplasmic pH gradients were not observed in the apical 20 μm of actively growing hyphae at the periphery of 18-h-old colonies. The cytoplasmic pH remained unchanged after prolonged growth in buffered medium with pH values between 2.5 or 9.5. Sudden changes in external pH significantly changed cytoplasmic pH by <1.3 pH units, but it returned to its original value within 20 min following treatment. The weak acid and antifungal food preservative sorbic acid caused prolonged, concentration-dependent intracellular acidification. The inhibition of ATPases with N-ethylmaleimide, dicychlohexylcarbodimide, or sodium azide caused the cytoplasmic pH to decrease by <1 pH unit. Treatment with the protonophore carbonyl cyanide m-chlorophenylhydrazone or cyanide p-(trifluoromethoxy) phenylhydrazone reduced the cytoplasmic pH by <1 pH unit. In older hyphae from 32-h-old cultures, RaVC became sequestered within large vacuoles, which were shown to have pH values between 6.2 and 6.5. Overall, our study demonstrates that RaVC is an excellent probe for visualizing and quantifying intracellular pH in living fungal hyphae.

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

scholarly journals Quantitative Imaging of Pre-mRNA Splicing Factors in Living Cells

2000 ◽  
Vol 11 (2) ◽  
pp. 413-418 ◽  
Author(s):  
Roland Eils ◽  
Daniel Gerlich ◽  
Wolfgang Tvaruskó ◽  
David L. Spector ◽  
Tom Misteli
Keyword(s):  
Quantitative Imaging ◽  
Living Cells ◽  
Mrna Splicing ◽  
Splicing Factors

Reversible Self-Assembly of Nanoprobes in Live Cells for Dynamic Intracellular pH Imaging

ACS Nano ◽  
2019 ◽  
Author(s):  
Bo Dong ◽  
Shuangli Du ◽  
Chunxiao Wang ◽  
Haohao Fu ◽  
Qiang Li ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Self Assembly ◽  
Live Cells ◽  
Ph Imaging

Intracellular pH-Sensitive PEG-block-Acetalated-Dextrans as Efficient Drug Delivery Platforms

2013 ◽  
Vol 5 (21) ◽  
pp. 10760-10766 ◽  
Author(s):  
Zhe Zhang ◽  
Xiaofei Chen ◽  
Li Chen ◽  
Shuangjiang Yu ◽  
Yue Cao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Intracellular Ph ◽  
Ph Sensitive

Use of a pH-sensitive fluorescent probe for measuring intracellular pH of Caco-2 cells

2007 ◽  
Vol 338 (1-2) ◽  
pp. 104-109 ◽  
Author(s):  
Earvin Liang ◽  
Puchun Liu ◽  
Steven Dinh
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Ph Sensitive
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