Use of a pH-sensitive fluorescent probe for measuring intracellular pH of Caco-2 cells

2007 ◽  
Vol 338 (1-2) ◽  
pp. 104-109 ◽  
Author(s):  
Earvin Liang ◽  
Puchun Liu ◽  
Steven Dinh
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Ph Sensitive

Construction of Polysiloxane-Based Fluorescent Probe for Visualizing The pH Down-regulation

2021 ◽  
Author(s):  
Xiaoni Wang ◽  
Zhiming Gou ◽  
Minggang Tian ◽  
Yujing Zuo
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Cell Apoptosis ◽  
Pathological Process ◽  
Down Regulation ◽  
Ph Values

Intracellular pH values change is a significant physiological and pathological process and plays vital roles in autophagy, self-repairing, and programmed cell apoptosis. A unique fluorescent probe (PN-1) based on polysiloxanes...

Light-activated “cycle-reversible intramolecular charge transfer” fluorescent probe: monitoring of pHi trace change induced by UV light in programmed cell death

2019 ◽  
Vol 55 (36) ◽  
pp. 5279-5282 ◽  
Author(s):  
Tao Jiang ◽  
Xin Wang ◽  
Ge Wang ◽  
Yafu Wang ◽  
Kui Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Charge Transfer ◽  
Programmed Cell Death ◽  
Synergistic Effect ◽  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Intramolecular Charge Transfer ◽  
Uv Light

A light-activated fluorescent probe shows cycle-reversible intramolecular charge transfer for different intracellular pH under the synergistic effect of protonation–deprotonation.

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

scholarly journals A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2679 ◽  
Author(s):  
Yibin Zhang ◽  
Jianheng Bi ◽  
Shuai Xia ◽  
Wafa Mazi ◽  
Shulin Wan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Solution Ph ◽  
Acidic Conditions ◽  
Ph Level ◽  
Rhodamine Dye

A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

Intracellular pH-Sensitive PEG-block-Acetalated-Dextrans as Efficient Drug Delivery Platforms

2013 ◽  
Vol 5 (21) ◽  
pp. 10760-10766 ◽  
Author(s):  
Zhe Zhang ◽  
Xiaofei Chen ◽  
Li Chen ◽  
Shuangjiang Yu ◽  
Yue Cao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Intracellular Ph ◽  
Ph Sensitive

Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

2017 ◽  
Vol 46 (40) ◽  
pp. 13957-13965 ◽  
Author(s):  
Shuoren Du ◽  
Javier Hernández-Gil ◽  
Hao Dong ◽  
Xiaoyu Zheng ◽  
Guangming Lyu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Ph Sensitive ◽  
Upconversion Nanoparticles ◽  
Imaging Probe ◽  
Ph Imaging ◽  
Ratiometric Probe ◽  
Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

A pH-sensitive multifunctional fluorescent probe based on N-annulated perylene for the sensitive and selective detection of hypochlorous acid

2017 ◽  
Vol 1 (11) ◽  
pp. 2292-2298 ◽  
Author(s):  
Xiao Zhang ◽  
Yongchao Yan ◽  
Qing Peng ◽  
Jian Wang ◽  
Yandi Hang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Samples ◽  
Hypochlorous Acid ◽  
Ph Sensitive ◽  
Selective Detection

The pH-sensitive multifunctional fluorescent probe PNPM based on N-annulated perylene was firstly developed for detecting HOCl selectively. And the probe could detect pH and HOCl simultaneously, and was successfully employed to detect HOCl in serum and water samples.

Sign in / Sign up

Export Citation Format

Share Document