Switchable polarity solvents for preconcentration and simultaneous determination of amino acids in human plasma samples

2018 ◽  
Vol 42 (12) ◽  
pp. 10007-10015 ◽  
Author(s):  
Fahimeh Zare ◽  
Mehrorang Ghaedi ◽  
Ramin Jannesar ◽  
Lobat Tayebi

The application of a mixture of amidine and alcohol as a switchable polarity solvent for the microextraction of 19 amino acids in biological samples.

2019 ◽  
Vol 169 ◽  
pp. 11-18 ◽  
Author(s):  
Davide Ferrari ◽  
Marco Ripa ◽  
Simone Premaschi ◽  
Giuseppe Banfi ◽  
Antonella Castagna ◽  
...  

2006 ◽  
Vol 29 (15) ◽  
pp. 2265-2283 ◽  
Author(s):  
Gorka Iriarte ◽  
Nerea Ferreirós ◽  
Izaskun Ibarrondo ◽  
Rosa Maria Alonso ◽  
Miren Itxaso Maguregi ◽  
...  

2020 ◽  
Vol 12 (7) ◽  
pp. 930-937 ◽  
Author(s):  
Erfan Barati ◽  
Naader Alizadeh

PPy-DBS was synthesized by an electrochemical process and used as a HS-SPME fiber for determination of antidepressants in plasma without derivatization steps.


2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.


2012 ◽  
Vol 38 (02) ◽  
pp. 178-184 ◽  
Author(s):  
Job Harenberg ◽  
Sandra Erdle ◽  
Svetlana Marx ◽  
Roland Krämer

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