Functionalised magnetic nanoparticles for uranium adsorption with ultra-high capacity and selectivity

Introduction: Further development of magnetic-based detection techniques could be of significant use in increasing the sensitivity of detection and quantification of hepatitis B virus (HBV) infection. The present work addresses the fabrication and characterization of a new bio-nano composite based on the immobilization of goat anti-HBsAg antibody on modified core-shell magnetic nanoparticles (NPs) by (3-aminopropyl) triethoxysilane (APTES), named Fe3O4@SiO2/NH2, and magnetic NPs modified by chitosan (Fe3O4@CS). Methods: At the first step, Fe3O4 was modified with the silica and APTES (Fe3O4@SiO2/NH2) and chitosan (Fe3O4@CS) separately. The goat anti-HBsAg antibody was activated by two different protocols: Sodium periodate and EDC-NHS. Then the resulted composites were conjugated with activated goat anti-HBsAg IgG. An external magnet collected Bio-super magnetic NPs (BSMNPs) and the remained solution was analyzed by the Bradford method to check the amount of attached antibody to the surface of BSMNPs. Results: The findings indicated that activation of antibodies by sodium periodate method 15-17 µg antibody immobilized on 1 mg of super magnetic nanoparticles (SMNPs). However, in the EDC-NHS method, 8-10 µg of antibody was conjugated with 1 mg of SMNPs. The resulting bio-magnetic NPs were applied for interaction with the HBsAg target using enzyme-linked immunosorbent assay (ELISA). About 1 µg antigen attached to 1 mg SMNPs, which demonstrated that the fabricated materials are applicable in the detection scope of HBsAg. Conclusion: In the present study, we developed new antibody-conjugated magnetic NPs for the detection of HBsAg using an efficient conjugation strategy. The results demonstrated that the binding capacity of Fe3O4@SiO2/NH2 was comparable with commercially available products. Our designed method for conjugating anti-HBsAg antibody to a magnetic nanoparticle opens the way to produce a high capacity of magnetic NPs.

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Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body

10.21203/rs.3.rs-68869/v2 ◽

2020 ◽

Author(s):

Robert Minkner ◽

Jian Xu ◽

Kenshin Takamura ◽

Jirayu Boonyakida ◽

Hermann Wätzig ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Recombinant Proteins ◽

Fat Body ◽

Binding Capacity ◽

Affinity Purification ◽

Expression System ◽

High Capacity ◽

Complex Matrix ◽

Special Equipment ◽

Promising Tool

Abstract Purification of recombinant proteins is often a challenging matter because high purity and high recovery are desired. If the expressed recombinant protein is also in a complex matrix, such as from the silkworm expression system, purification becomes more challenging. Even if purification from the silkworm expression system is troublesome, it benefits from a high capacity for the production of recombinant proteins. In this study, magnetic nanoparticles (MNPs) were investigated as a suitable tool for the purification of proteins from the complex matrix of the silkworm fat body. The MNPs were modified with nickel so that they have an affinity for His-tagged proteins, as the MNP purification protocol itself does not need special equipment except for a magnet. Among the three different kinds of investigated MNPs, MNPs with sizes of 100 nm to 200 nm and approximately 20 nm-thick nickel shells were the most suitable for our purpose. With them, the total protein amount was reduced by up to at least approximately 77.7%, with a protein recovery of around 50.8% from the silkworm fat body. The minimum binding capacity was estimated to be 83.3 µg protein/mg MNP. Therefore, these MNPs are a promising tool as a purification pretreatment of complex sample matrices.

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Ni-modified Magnetic Nanoparticles for Affinity Purification of His-tagged Proteins From the Complex Matrix of the Silkworm Fat Body

10.21203/rs.3.rs-68869/v1 ◽

2020 ◽

Author(s):

Robert Minkner ◽

Jian Xu ◽

Kenshin Takamura ◽

Jirayu Boonyakida ◽

Hermann Wätzig ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Recombinant Proteins ◽

Fat Body ◽

Binding Capacity ◽

Affinity Purification ◽

Expression System ◽

High Capacity ◽

Complex Matrix ◽

Special Equipment ◽

Modified Magnetic Nanoparticles

Abstract Purification of recombinant proteins is often a challenging matter because high purity and high recovery are desired. If the expressed recombinant protein is also in a complex matrix, such as a silkworm expression system, purification becomes more challenging. Even if purification from the silkworm expression system is troublesome, it has the benefit of a high capacity for production of recombinant proteins. In this study, we investigated magnetic nanoparticles (MNPs) as a suitable tool for the purification of proteins from the complex matrix of the silkworm fat body. We modified MNPs with nickel so that they have an affinity for His-tagged proteins, as the MNP purification protocol does not need special equipment except for a magnet. Among the three different kinds of MNPs we investigated, MNPs with sizes of 100 nm to 200 nm and approximately 20 nm-thick nickel shells were the most suitable for our purpose. We reduced the total protein amount by up to 77.7%, with a protein recovery of 50.8% from the silkworm fat body. The minimum binding capacity was estimated to be 83.3 µg protein/mg MNP.

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Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body

Journal of Nanobiotechnology ◽

10.1186/s12951-020-00715-1 ◽

2020 ◽

Vol 18 (1) ◽

Author(s):

Robert Minkner ◽

Jian Xu ◽

Kenshin Takemura ◽

Jirayu Boonyakida ◽

Hermann Wätzig ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Recombinant Proteins ◽

Fat Body ◽

Binding Capacity ◽

Affinity Purification ◽

Expression System ◽

High Capacity ◽

Complex Matrix ◽

Special Equipment ◽

Promising Tool

Abstract Purification of recombinant proteins is often a challenging matter because high purity and high recovery are desired. If the expressed recombinant protein is also in a complex matrix, such as from the silkworm expression system, purification becomes more challenging. Even if purification from the silkworm expression system is troublesome, it benefits from a high capacity for the production of recombinant proteins. In this study, magnetic nanoparticles (MNPs) were investigated as a suitable tool for the purification of proteins from the complex matrix of the silkworm fat body. The MNPs were modified with nickel so that they have an affinity for His-tagged proteins, as the MNP purification protocol itself does not need special equipment except for a magnet. Among the three different kinds of investigated MNPs, MNPs with sizes of 100 nm to 200 nm and approximately 20 nm-thick nickel shells were the most suitable for our purpose. With them, the total protein amount was reduced by up to at least approximately 77.7%, with a protein recovery of around 50.8% from the silkworm fat body. The minimum binding capacity was estimated to be 83.3 µg protein/mg MNP. Therefore, these MNPs are a promising tool as a purification pretreatment of complex sample matrices.

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Facile preparation of boronic acid-functionalized magnetic nanoparticles with a high capacity and their use in the enrichment ofcis-diol-containing compounds from plasma

Biomedical Chromatography ◽

10.1002/bmc.3277 ◽

2014 ◽

Vol 29 (2) ◽

pp. 312-320 ◽

Cited By ~ 21

Author(s):

Jin Du ◽

Maofang He ◽

Xiaomeng Wang ◽

Hua Fan ◽

Yinmao Wei

Keyword(s):

Magnetic Nanoparticles ◽

Boronic Acid ◽

High Capacity ◽

Functionalized Magnetic Nanoparticles ◽

Facile Preparation

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Constructing Carbon-Coated Fe3O4 Hierarchical Microstructures with a Porous Structure and their Excellent Cr(VI) Ion Removal Properties

10.21203/rs.3.rs-335739/v1 ◽

2021 ◽

Author(s):

xiaoping wu ◽

Lin Cheng ◽

Chang-Sheng Song ◽

Yi-Zhe Zhang ◽

Xiao-Jing Shi ◽

...

Keyword(s):

Aqueous Solution ◽

Magnetic Nanoparticles ◽

Solvothermal Method ◽

Hollow Spheres ◽

High Capacity ◽

Hollow Structure ◽

Magnetic Composite ◽

Ion Removal ◽

Carbon Coated ◽

Facile Solvothermal Method

Abstract Here, a facile solvothermal method coupled with an annealing strategy is developed to synthesize Fe3O4/carbon (Fe3O4@C) magnetic composite microstructures with different morphologies, including flower-like, hollow spheres and egg-like. Owing to the unique multi-porous and hollow structure, the as-prepared hierarchical Fe3O4@C hollow microspheres composite exhibit appealing performance as an absorbent of Cr(VI) ions in aqueous solution, delivering a high capacity of ca.197.2mg/g. Furthermore, the magnetic Fe3O4 ‘‘core’’ in composite hierarchical microstructures makes them easy to separate from aqueous systems by magnetic separation, the layer of carbon effectively prevents agglomerations of magnetic nanoparticles and expands their range of applications. The excellent Cr(VI) ions adsorbent activities of the Fe3O4@C magnetic composite microstructures would have a potential adsorbing material application in environmental purification.

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Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

Pharmaceutics ◽

10.3390/pharmaceutics12090854 ◽

2020 ◽

Vol 12 (9) ◽

pp. 854

Author(s):

Hai Doan Do ◽

Christine Ménager ◽

Aude Michel ◽

Johanne Seguin ◽

Tawba Korichi ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Nucleic Acid ◽

High Efficiency ◽

High Capacity ◽

Cationic Liposomes ◽

Nucleic Acid Delivery ◽

Image Guided ◽

Liposome Preparation ◽

Magnetic Resonance Imaging Contrast ◽

Diagnostic Agent

Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities r2 and r1 was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy.

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