Carbon nanodot impregnated fluorescent nanofibers for in vivo monitoring and accelerating full-thickness wound healing

2017 ◽  
Vol 5 (32) ◽  
pp. 6645-6656 ◽  
Author(s):  
Pallabi Pal ◽  
Bodhisatwa Das ◽  
Prabhash Dadhich ◽  
Arun Achar ◽  
Santanu Dhara
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Noninvasive Monitoring ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Full Thickness ◽  
In Vivo Monitoring ◽  
Full Thickness Wound

Development of an intrinsically fluorescent nanofibrous scaffold of polycaprolactone–gelatin for skin tissue regeneration and noninvasive monitoring of scaffold activity in vivo.

Bioactive antibacterial bilayer PCL/gelatin nanofibrous scaffold promotes full-thickness wound healing

2020 ◽  
Vol 583 ◽  
pp. 119413 ◽  
Author(s):  
Arman Jafari ◽  
Armin Amirsadeghi ◽  
Shadi Hassanajili ◽  
Negar Azarpira
Keyword(s):  
Wound Healing ◽  
Nanofibrous Scaffold ◽  
Full Thickness ◽  
Full Thickness Wound

Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

2020 ◽  
pp. 153473462092162
Author(s):  
T. S. Shanmugarajan ◽  
N. Kalai Selvan ◽  
Varuna Naga Venkata Arjun Uppuluri
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Macrophage Polarization ◽  
Negative Control ◽  
Skin Tissue ◽  
Self Healing ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Tissue Defects

Full-thickness burns pose a major challenge for clinicians to handle because of their restricted self-healing ability. Even though several approaches have been implemented for repairing these burnt skin tissue defects, all of them had unsatisfactory outcomes. Moreover, during recent years, skin tissue engineering techniques have emerged as a promising approach to improve skin tissue regeneration and overcome the shortcomings of the traditional approaches. Although previous literatures report the wound healing effects of the squalene oil, in the current study, for the first time, we developed a squalene-loaded emulgel-based scaffold as a novel approach for potential skin regeneration. This squalene-loaded agar-based emulgel scaffold was fabricated by using physical cross-linking technique using lecithin as an emulsifier. Characterization studies such as X-ray diffraction, Fourier-transform infrared spectroscopy, and field emission scanning electron microscopy revealed the amorphous nature, chemical interactions, and cross-linked capabilities of the developed emulgel scaffold. The squalene-loaded emulgel scaffold showed excellent wound contraction when compared with the agar gel and negative control. In case of the histopathology and recent immunohistochemistry findings, it was clearly evidenced that squalene-loaded emulgel promoted faster rate of the revascularization and macrophage polarization in order to enhance the burn wound healing. Moreover, the findings also revealed that the incorporation of squalene oil into the formulation enhances collagen deposition and accelerates the burnt skin tissue regeneration process. Finally, we conclude that the squalene-loaded emulgel scaffold could be an effective formulation used in the treatment of the burnt skin tissue defects.

Upregulating mTOR/ERK signaling with leonurine for promoting angiogenesis and tissue regeneration in a full-thickness cutaneous wound model

2018 ◽  
Vol 9 (4) ◽  
pp. 2374-2385 ◽  
Author(s):  
Chenggui Wang ◽  
Zengjie Zhang ◽  
Tianzhen Xu ◽  
Yiting Lou ◽  
Qingqing Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Healing Process ◽  
Erk Signaling ◽  
Wound Healing Process ◽  
Erk Pathway ◽  
Full Thickness ◽  
Cutaneous Wound ◽  
Wound Model

LN promoted the angiogenesis of endothelial cells by activating the mTOR/ERK pathway, and efficiently enhanced the wound-healing processin vivo.

Fabrication of sliver nanoparticles/polyvinyl alcohol/gelatin ternary nanofiber mats for wound healing application

2020 ◽  
Vol 35 (2) ◽  
pp. 287-298
Author(s):  
Somaya Amer ◽  
Noha Attia ◽  
Samir Nouh ◽  
Mahmoud El-Kammar ◽  
Ahmed Korittum ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Wound Dressings ◽  
Full Thickness ◽  
Microbial Invasion ◽  
Thickness Skin ◽  
Nanofiber Mats ◽  
Full Thickness Wound

Purpose In this study, we aimed to determine the regenerative and antimicrobial impact of the electrospun nanofiber mats, with/without silver nanoparticles (AgNPs), on full-thickness skin wounds in rabbits. Methods Polyvinyl alcohol was combined with gelatin to provide biocompatible electrospun binary nanofiber mats. AgNPs were added to the polyvinyl alcohol/gelatin mixture to obtain ternary nanofiber-AgNPs mats. Binary and ternary nanofiber mats were characterized by scanning electron microscopy before being applied as wound dressings in vivo. Subsequently, wound healing was evaluated. Results Both nanofiber/nanofiber-AgNPs mats improved the microscopic quality of the healed skin, albeit without obvious acceleration of the healing rate. As well, both types of nanofiber mats were able to combat microbial invasion into the wound bed. Conclusions Both binary polyvinyl alcohol/gelatin and ternary polyvinyl alcohol/gelatin/AgNPs nanofiber mats developed in the present study depicted similar regenerative and antimicrobial potential when applied as full-thickness wound dressing. However, in comparison to the binary nanofiber mats, no obvious synergistic effect was observed after loading nanofibers with AgNPs.

In Vivo Studies of 3D Starch–Gelatin Scaffolds for Full-Thickness Wound Healing

2020 ◽  
Vol 3 (5) ◽  
pp. 2920-2929 ◽  
Author(s):  
Roha Chhabra ◽  
Vaibhavi Peshattiwar ◽  
Tejal Pant ◽  
Aparna Deshpande ◽  
Deepak Modi ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Studies ◽  
Full Thickness ◽  
Full Thickness Wound

scholarly journals Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 854
Author(s):  
Ahmad Hivechi ◽  
Peiman Brouki Milan ◽  
Khashayar Modabberi ◽  
Moein Amoupour ◽  
Kaveh Ebrahimzadeh ◽  
...  
Keyword(s):  
Cell Activity ◽  
Average Diameter ◽  
Skin Substitute ◽  
Full Thickness ◽  
Skin Integrity ◽  
Hematoxylin And Eosin ◽  
First Time ◽  
Full Thickness Wound

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

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