Fluorescent Wittig reagent as a novel ratiometric probe for the quantification of 5-formyluracil and its application in cell imaging

In this study, for the first time, a novel near-infrared and ratiometric fluorescent probe was conveniently synthesized by reacting PDI-based salicylaldehyde with 2-(hydrazonomethyl)phenol.

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Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

Current Analytical Chemistry ◽

10.2174/1573411014666180904104629 ◽

2018 ◽

Vol 15 (1) ◽

pp. 47-55

Author(s):

Xuebing Li ◽

Haifen Yang ◽

Ning Wang ◽

Tijian Sun ◽

Wei Bian ◽

...

Keyword(s):

Microwave Heating ◽

Fluorescent Probe ◽

Fluorescence Intensity ◽

Carbon Dots ◽

Cell Imaging ◽

Water Soluble ◽

Heating Process ◽

X Ray ◽

Doped Carbon Dots ◽

Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

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Engineering dithiobenzoic acid lactone-decorated Si-rhodamine as a highly selective near-infrared HOCl fluorescent probe for imaging drug-induced acute nephrotoxicity

Chemical Communications ◽

10.1039/c9cc04736k ◽

2019 ◽

Vol 55 (73) ◽

pp. 10916-10919 ◽

Cited By ~ 17

Author(s):

Jinping Wang ◽

Dan Cheng ◽

Longmin Zhu ◽

Peng Wang ◽

Hong-Wen Liu ◽

...

Keyword(s):

Fluorescent Probe ◽

Near Infrared ◽

Drug Induced ◽

Acid Lactone ◽

First Time

A NIR fluorescent probe based on Si-rhodamine dithiobenzoic acid lactone was used to selectively visualize HOCl during GEN-induced nephrotoxicity for the first time.

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A pH tuning single fluorescent probe based on naphthalene for dual-analytes (Mg2+ and Al3+) and its application in cell imaging

RSC Advances ◽

10.1039/d0ra02101f ◽

2020 ◽

Vol 10 (36) ◽

pp. 21399-21405

Author(s):

Chunwei Yu ◽

Shuhua Cui ◽

Yuxiang Ji ◽

Shaobai Wen ◽

Li Jian ◽

...

Keyword(s):

Schiff Base ◽

Fluorescent Probe ◽

Cell Imaging ◽

Quantitative Detection

In this study, a naphthalene Schiff-base P which serves as a dual-analyte probe for the quantitative detection of Al3+ and Mg2+ has been designed.

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Vanillin-coumarin hybrid molecule as an efficient fluorescent probe for trace level determination of Hg(II) and its application in cell imaging

Talanta ◽

10.1016/j.talanta.2011.06.073 ◽

2011 ◽

Vol 85 (3) ◽

pp. 1658-1664 ◽

Cited By ~ 18

Author(s):

Subarna Guha ◽

Sisir Lohar ◽

Ipsit Hauli ◽

Subhra K. Mukhopadhyay ◽

Debasis Das

Keyword(s):

Fluorescent Probe ◽

Cell Imaging ◽

Hybrid Molecule ◽

Trace Level ◽

Trace Level Determination

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I2 catalyzed access of spiro[indoline-3,4′-pyridine] appended amine dyad: new ON–OFF chemosensors for Cu2+ and imaging in living cells

Organic & Biomolecular Chemistry ◽

10.1039/c7ob02651j ◽

2018 ◽

Vol 16 (2) ◽

pp. 302-315 ◽

Cited By ~ 8

Author(s):

Animesh Mondal ◽

Barnali Naskar ◽

Sanchita Goswami ◽

Chandraday Prodhan ◽

Keya Chaudhuri ◽

...

Keyword(s):

Fluorescent Probe ◽

Hepg2 Cells ◽

Cell Imaging ◽

Colorimetric Detection ◽

Living Cells ◽

Fluorescent Cell

An efficient, easily tuneable route to construct a structurally diverse organic fluorescent probe and its applications towards the colorimetric detection of Cu2+ ions and in vitro fluorescent cell imaging of Cu2+ in HepG2 cells.

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Live-Cell Imaging of Caspase Activation for High-Content Screening

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057109343207 ◽

2009 ◽

Vol 14 (8) ◽

pp. 956-969 ◽

Cited By ~ 31

Author(s):

Christophe Antczak ◽

Toshimitsu Takagi ◽

Christina N. Ramirez ◽

Constantin Radu ◽

Hakim Djaballah

Keyword(s):

Cell Death ◽

Live Cell Imaging ◽

Cell Imaging ◽

Exposure Period ◽

Live Cells ◽

Assay Method ◽

Caspase Activation ◽

Fluorogenic Substrate ◽

Automated Imaging ◽

First Time

Caspases are central to the execution of programmed cell death, and their activation constitutes the biochemical hallmark of apoptosis. In this article, the authors report the successful adaptation of a high-content assay method using the DEVDNucView488™ fluorogenic substrate, and for the first time, they show caspase activation in live cells induced by either drugs or siRNA. The fluorogenic substrate was found to be nontoxic over an exposure period of several days, during which the authors demonstrate automated imaging and quantification of caspase activation of the same cell population as a function of time. Overexpression of the antiapoptotic protein Bcl-XL, alone or in combination with the inhibitor Z-VAD-FMK, attenuated caspase activation in HeLa cells exposed to doxorubicin, etoposide, or cell death siRNA. This method was further validated against 2 well-characterized NSCLC cell lines reported to be sensitive (H3255) or refractory (H2030) to erlotinib, where the authors show a differential time-dependent activation was observed for H3255 and no significant changes in H2030, consistent with their respective chemosensitivity profile. In summary, the results demonstrate the feasibility of using this newly adapted and validated high-content assay to screen chemical or RNAi libraries for the identification of previously uncovered enhancers and suppressors of the apoptotic machinery in live cells. ( Journal of Biomolecular Screening 2009:956-969)

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