scholarly journals Experimental and theoretical approaches for the selective detection of thymine in real samples using gold nanoparticles as a biochemical sensor

RSC Advances ◽  
2018 ◽  
Vol 8 (43) ◽  
pp. 24328-24337 ◽  
Author(s):  
Kamlesh Shrivas ◽  
Nidhi Nirmalkar ◽  
Santosh Singh Thakur ◽  
Ramsingh Kurrey ◽  
Deepak Sinha ◽  
...  

We report a simple, selective and cost effective method for the qualitative and quantitative determination of thymine in a DNA standard and in urine samples using gold nanoparticles (AuNPs) as a label-free colorimetric biochemical sensor.

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Haliza Katas ◽  
Noor Zianah Moden ◽  
Chei Sin Lim ◽  
Terence Celesistinus ◽  
Jie Yee Chan ◽  
...  

Biosynthesized or biogenic metallic nanoparticles, particularly silver and gold nanoparticles (AgNPs and AuNPs, respectively), have been increasingly used because of their advantages, including high stability and loading capacity; moreover, these nanoparticles are synthesized using a green and cost-effective method. Previous studies have investigated reducing and/or stabilizing agents from various biological sources, including plants, microorganisms, and marine-derived products, using either a one-pot or a multistep process at different conditions. In addition, extensive studies have been performed to determine the biological or pharmacological effects of these nanoparticles, such as antimicrobial, antitumor, anti-inflammatory, and antioxidant effects. In the recent years, chitosan, a natural cationic polysaccharide, has been increasingly investigated as a reducing and/or stabilizing agent in the synthesis of biogenic metallic nanoparticles with potential applications in nanomedicine. Here, we have reviewed the mechanism of biosynthesis and potential applications of AgNPs and AuNPs and their chitosan-mediated nanocomposites in nanomedicine.


2018 ◽  
Vol 5 (10) ◽  
pp. 181359 ◽  
Author(s):  
Samah Abo El Abass ◽  
Heba Elmansi

A green, sensitive and cost-effective method is introduced in this research for the determination of bambuterol and its main degradation product, terbutaline, simultaneously, relying on the synchronous spectrofluorimetric technique. First derivative synchronous spectrofluorimetric amplitude is measured at Δ λ = 20 nm, so bambuterol can be quantitated at 260 nm, and terbutaline can be measured at 290 nm, each at the zero crossing point of the other. The amplitude–concentration plots were linear over the concentration ranges of 0.2–6.0 µg ml −1 and 0.2–4.0 µg ml −1 for both bambuterol and terbutaline, respectively. Official guidelines were followed to calculate the validation parameters of the proposed method. The low values of limits of detection of 0.023, 0.056 µg ml −1 and limits of quantitation of 0.071, 0.169 µg ml −1 for bambuterol and terbutaline, respectively, point to the sensitivity of the method. Bambuterol is a prodrug for terbutaline, and the latter is considered its degradation product so the established method could be regarded as a stability-indicating one. Moreover, the proposed method was used for the analysis of bambuterol and terbutaline in their single ingredient preparations and the results revealed statistical agreement with the reference method. The suggested method, being a simple and low-cost procedure, is superior to the previously published methods which need more sophisticated techniques, longer analysis time and highly toxic solvents and reagents. It could be considered as an eco-friendly analytical procedure.


2021 ◽  
Vol 34 (1) ◽  
pp. 41-57
Author(s):  
Beeta Rani Khalkho ◽  
Anushree Saha ◽  
Bhuneshwari Sahu ◽  
Manas Kanti Deb

Abstract. The purpose of the present research was to design a method for the colorimetric determination of L-cysteine. We have employed PVA capped gold nanoparticles (GNPs) as a probe. The as-synthesized GNPs were further characterized by UV-vis absorption spectroscopy, transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and Zeta potential analyser. The results show that the presence of L-cysteine caused the quenching of the surface plasmon resonance band of the GNPs at 524 nm. It was accompanied by the appearance of a new absorbance of a new absorbance band at 670 nm. The color of the colloidal GNPs changed from wine red to blue. The change in color of the GNPs was due to their aggregation induced by the presence of L-cysteine. Based on these observations, the as-synthesized GNPs were utilized to develop a novel colorimetric sensor for L-cysteine detection in food samples. Significantly, other biomolecules such as alanine, proline, phenylalanine, tryptophane, valine, arginine, glutamic acid, lysine and histidine did not cause any change in the color of the GNPs solutions. This colorimetric probe showed excellent selectivity and high sensitivity for L-cysteine with a detection limit of 2.0 μg mL-1.


2018 ◽  
Vol 6 (48) ◽  
pp. 8214-8220 ◽  
Author(s):  
Quan Li ◽  
Kaite Peng ◽  
Yanzhen Lu ◽  
Aoxin Li ◽  
Fenfang Che ◽  
...  

Label-free fluorescent ionic liquid-functionalized silicon nanoparticles with tunable amphiphilicity for highly sensitive and selective detection of Hg2+ were synthesized.


2012 ◽  
Vol 4 (10) ◽  
pp. 3193 ◽  
Author(s):  
Vladimir V. Apyari ◽  
Stanislava G. Dmitrienko ◽  
Viktoriya V. Arkhipova ◽  
Aydar G. Atnagulov ◽  
Yuri A. Zolotov

2011 ◽  
Vol 3 (6) ◽  
pp. 1405 ◽  
Author(s):  
Abdolkarim Abbaspour ◽  
Hamed Valizadeh ◽  
Abdolreza Khajehzadeh

2017 ◽  
Vol 9 (4) ◽  
pp. 716-723 ◽  
Author(s):  
Nafiseh Fahimi-Kashani ◽  
Ali Rashti ◽  
M. Reza Hormozi-Nezhad ◽  
Vahideh Mahdavi

A reliable, simple, and sensitive fluorescence method was developed for the determination of methyl parathion (MP) in rice using MoS2 quantum dots (QDs).


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