A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes

Extra-synaptic exocytosis is an essential component of cellular communication. A knowledge gap exists in the exocytosis of the non-redox active transmitter acetylcholine. Using the nano-interface between two immiscible electrolyte solutions and scanning electrochemical microscopy, a high resolution spatiotemporal study of acetylcholine exocytosis is shown from individual neuronal soma.

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Scanning Electrochemical Microscopy. 30. Application of Glass Micropipet Tips and Electron Transfer at the Interface between Two Immiscible Electrolyte Solutions for SECM Imaging

Analytical Chemistry ◽

10.1021/ac00113a011 ◽

1995 ◽

Vol 67 (17) ◽

pp. 2787-2790 ◽

Cited By ~ 28

Author(s):

Theodros. Solomon ◽

Allen J. Bard

Keyword(s):

Electron Transfer ◽

Scanning Electrochemical Microscopy ◽

Electrolyte Solutions ◽

Electrochemical Microscopy

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Nanoscale mapping of catalytic hotspots on Fe, N-modified HOPG by scanning electrochemical microscopy-atomic force microscopy

Nanoscale ◽

10.1039/c8nr00849c ◽

2018 ◽

Vol 10 (15) ◽

pp. 6962-6970 ◽

Cited By ~ 9

Author(s):

Srikanth Kolagatla ◽

Palaniappan Subramanian ◽

Alex Schechter

Keyword(s):

Atomic Force Microscopy ◽

Surface Modification ◽

High Resolution ◽

Scanning Electrochemical Microscopy ◽

Graphitic Carbon ◽

Force Microscopy ◽

Atomic Force ◽

Electrochemical Microscopy

The scanning electrochemical microscopy-atomic force microscopy (SECM-AFM) technique is used to map catalytic currents post Fe and N surface modification of graphitic carbon with an ultra-high resolution of 50 nm.

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Characterization of Microfabricated Probes for Combined Atomic Force and High-Resolution Scanning Electrochemical Microscopy

Analytical Chemistry ◽

10.1021/ac0521495 ◽

2006 ◽

Vol 78 (15) ◽

pp. 5436-5442 ◽

Cited By ~ 47

Author(s):

Maurizio R. Gullo ◽

Patrick L. T. M. Frederix ◽

Terunobu Akiyama ◽

Andreas Engel ◽

Nico F. deRooij ◽

...

Keyword(s):

High Resolution ◽

Scanning Electrochemical Microscopy ◽

Atomic Force ◽

Electrochemical Microscopy

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Quantifying microbial chatter: scanning electrochemical microscopy as a tool to study interactions in biofilms

Proceedings of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rspa.2018.0405 ◽

2018 ◽

Vol 474 (2220) ◽

pp. 20180405 ◽

Cited By ~ 7

Author(s):

Sophie E. Darch ◽

Dipankar Koley

Keyword(s):

Scanning Electrochemical Microscopy ◽

Emergent Properties ◽

Spatial Structures ◽

Natural Habitats ◽

Redox Active ◽

Primary Means ◽

Microbe Interactions ◽

By Products ◽

Electrochemical Microscopy ◽

Chemical Mediators

Bacteria are often found in their natural habitats as spatially organized biofilm communities. While it is clear from recent work that the ability to organize into precise spatial structures is important for fitness of microbial communities, a significant gap exists in our understanding regarding the mechanisms bacteria use to adopt such physical distributions. Bacteria are highly social organisms that interact, and it is these interactions that have been proposed to be critical for establishing spatially structured communities. A primary means by which bacteria interact is via small, diffusible molecules including dedicated signals and metabolic by-products; however, quantitatively monitoring the production of these molecules in time and space with the micron-scale resolution required has been challenging. In this perspective, scanning electrochemical microscopy (SECM) is discussed as a powerful tool to study microbe–microbe interactions through the detection of small redox-active molecules. We highlight SECM as a means to quantify and spatially resolve the chemical mediators of bacterial interactions and begin to elucidate the mechanisms used by bacteria to regulate the emergent properties of biofilms.

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