Automated sample preparation and GC-API-MS/MS as a powerful tool for analysis of legacy POPs in human serum and plasma

2020 ◽  
Vol 12 (7) ◽  
pp. 912-929
Author(s):  
Sandra Huber ◽  
Maria Averina ◽  
Jan Brox
Keyword(s):  
Sample Preparation ◽  
Polychlorinated Biphenyls ◽  
Human Serum ◽  
Organochlorine Pesticides ◽  
Organic Pollutants ◽  
High Throughput ◽  
Persistent Organic Pollutants ◽  
Polybrominated Diphenylethers ◽  
Automated Sample Preparation ◽  
Spe Method

An automated high-throughput SPE method for selected persistent organic pollutants comprising 17 organochlorine pesticides, 22 polychlorinated biphenyls, 9 polybrominated diphenylethers and octachlorostyrene in human serum and plasma was developed.

High throughput method for the determination of organochlorine pesticides and polychlorinated biphenyls in human serum

2007 ◽  
Vol 852 (1-2) ◽  
pp. 15-21 ◽  
Author(s):  
Fernando Goñi ◽  
Raul López ◽  
Arsenio Etxeandia ◽  
Esmeralda Millán ◽  
Pilar Amiano
Keyword(s):  
Polychlorinated Biphenyls ◽  
Human Serum ◽  
Organochlorine Pesticides ◽  
High Throughput ◽  
High Throughput Method

scholarly journals «Грязная дюжина» Стокгольмской конвенции. Химия и токсикология стойких органических загрязняющих веществ (СОЗ): обзор литературы

2020 ◽  
pp. 12-61
Author(s):  
В.Ю. Цыганков
Keyword(s):  
Polychlorinated Biphenyls ◽  
Organochlorine Pesticides ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Chemical Properties ◽  
Stockholm Convention ◽  
Physical And Chemical Properties ◽  
Dirty Dozen ◽  
Physical And Chemical ◽  
And Chemical Properties

В главе представлена информация о Стокгольмской конвенции, этапах ее формирования и "грязной дюжине". Описаны физико-химические свойства, распределение в окружающей среде, метаболизм и деградация, токсичность основных стойких органических загрязняющих веществ (СОЗ), таких как хлорорганические пестициды (ХОП) и полихлорированные бифенилы (ПХБ). Ключевые слова: «грязная дюжина»; Стокгольмская конвенция; СОЗ; ХОП; ПХБ; физико-химические свойства; распределение в окружающей среде; метаболизм и деградация; токсичность. In the chapter, the "dirty dozen" of the Stockholm Convention and the stages of its formation are presented. The physical and chemical properties, distribution in the environment, metabolism and degradation, toxicity of the main persistent organic pollutants (POPs), such as organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), are described. Keywords: "dirty dozen"; Stockholm Convention; POPs; OCP; PCBs; physical and chemical properties; distribution in the environment; metabolism and deg-radation; toxicity.

scholarly journals Obesity and Persistent Organic Pollutants: Possible Obesogenic Effect of Organochlorine Pesticides and Polychlorinated Biphenyls

Obesity ◽  
2011 ◽  
Vol 19 (4) ◽  
pp. 709-714 ◽  
Author(s):  
Eveline Dirinck ◽  
Philippe G. Jorens ◽  
Adrian Covaci ◽  
Tinne Geens ◽  
Laurence Roosens ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Organochlorine Pesticides ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants

scholarly journals Automated Sample Preparation and Data Collection Workflow for High-Throughput In Vitro Metabolomics

Metabolites ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 52
Author(s):  
Julia M. Malinowska ◽  
Taina Palosaari ◽  
Jukka Sund ◽  
Donatella Carpi ◽  
Gavin R. Lloyd ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
High Throughput Screening ◽  
In Vitro Screening ◽  
Chemical Safety ◽  
Experimental Conditions ◽  
Well Location ◽  
Relative Standard ◽  
Automated Sample Preparation

Regulatory bodies have started to recognise the value of in vitro screening and metabolomics as two types of new approach methodologies (NAMs) for chemical risk assessments, yet few high-throughput in vitro toxicometabolomics studies have been reported. A significant challenge is to implement automated sample preparation of the low biomass samples typically used for in vitro screening. Building on previous work, we have developed, characterised and demonstrated an automated sample preparation and analysis workflow for in vitro metabolomics of HepaRG cells in 96-well microplates using a Biomek i7 Hybrid Workstation (Beckman Coulter) and Orbitrap Elite (Thermo Scientific) high-resolution nanoelectrospray direct infusion mass spectrometry (nESI-DIMS), across polar metabolites and lipids. The experimental conditions evaluated included the day of metabolite extraction, order of extraction of samples in 96-well microplates, position of the 96-well microplate on the instrument’s deck and well location within a microplate. By using the median relative standard deviation (mRSD (%)) of spectral features, we have demonstrated good repeatability of the workflow (final mRSD < 30%) with a low percentage of features outside the threshold applied for statistical analysis. To improve the quality of the automated workflow further, small method modifications were made and then applied to a large cohort study (4860 sample infusions across three nESI-DIMS assays), which confirmed very high repeatability of the whole workflow from cell culturing to metabolite measurements, whilst providing a significant improvement in sample throughput. It is envisioned that the automated in vitro metabolomics workflow will help to advance the application of metabolomics (as a part of NAMs) in chemical safety, primarily as an approach for high throughput screening and prioritisation.

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