Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2136-2153 ◽  
Author(s):  
Glen Kelp ◽  
Joy Li ◽  
Junlan Lu ◽  
Nicholas DiNapoli ◽  
Robert Delgado ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Live Cells ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy ◽  
Flowing Solution

Microfluidics, dielectrophoresis (DEP) and metasurface-enhanced infrared reflection spectroscopy (MEIRS) are combined for capturing and rapid spectroscopy of live cells.

scholarly journals Nanoplasmonic sensor for monitoring the effects of chemical stimuli on live cells using metasurface-enhanced infrared reflection spectroscopy

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Steven H. Huang ◽  
Jiaruo Li ◽  
Zhiyuan Fan ◽  
Robert Delgado ◽  
Gennady Shvets
Keyword(s):  
Infrared Spectroscopy ◽  
Biological Materials ◽  
Live Cells ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Chemical Stimuli ◽  
Infrared Reflection Spectroscopy

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures – plasmonic metasurfaces – as substrates for metasurface-enhanced...

Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy

2021 ◽  
Author(s):  
Steven H. Huang ◽  
Jiaruo Li ◽  
Zhiyuan Fan ◽  
Robert Delgado ◽  
Gennady Shvets
Keyword(s):  
Intracellular Signaling ◽  
Strong Field ◽  
Detection System ◽  
Field Enhancement ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures, or plasmonic metasurfaces, as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.

Noncontact infrared reflection spectroscopy using optical fibers

1994 ◽  
Vol 65 (8) ◽  
pp. 2731-2732 ◽  
Author(s):  
D. Bunimovich ◽  
E. Belotserkovsky ◽  
A. Katzir
Keyword(s):  
Optical Fibers ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy
Sign in / Sign up

Export Citation Format

Share Document