scholarly journals An improved ASM-GDA approach to evaluate the production kinetics of loosely bound and tightly bound extracellular polymeric substances in biological phosphorus removal process

RSC Advances ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2495-2506
Author(s):  
Hai Cui ◽  
Shan-Shan Yang ◽  
Ji-Wei Pang ◽  
Hai-Rong Mi ◽  
Chen-Chen Nuer ◽  
...  

An extended activated sludge model no. 2 provides a new recognition of the contributions of both loosely- and tightly-bound EPS into phosphorus removal by incorporating their formation and degradation processes during the anaerobic–aerobic cycle.

1998 ◽  
Vol 37 (4-5) ◽  
pp. 481-484 ◽  
Author(s):  
Mamoru Kawaharasaki ◽  
Takahiro Kanagawa ◽  
Hideo Tanaka ◽  
Kazunori Nakamura

A 16S rRNA-targeted oligonucletide probe (MP2) specific for the phosphate-accumulating bacterium (PAB) M. phosphovorus was designed and applied to a sludge from an enhanced biological phosphorus removal (EBPR) process. Probes specific for defined phylogenetic groups and the polyphosphate staining dye, DAPI (4′, 6-diamidino-2-phenylindol dihydrochloride) were also used to analyze the activated sludge community. M. phosphovorus was about 3% of the total bacteria in the EBPR sludge used. Proteobacteria belonging to the beta subclass were the most abundant. Many coccoid bacteria similar to M. phosphovorus were stained with DAPI. The percentage of PABs detected by DAPI stain was about 9% of the total bacteria.


2001 ◽  
Vol 44 (1) ◽  
pp. 49-56 ◽  
Author(s):  
M. Wichern ◽  
F. Obenaus ◽  
P. Wulf ◽  
K.-H. Rosenwinkel

In 1999 the Activated Sludge Model no. 3 (ASM 3) by the IWA task Group on Mathematical Modeling for Design and Operation of Biological Wastewater Treatment was presented. The model is used for simulation of nitrogen removal. On the basis of a new calibration of the ASM 3 with the easy degradable COD measured by respiration simulation runs of this paper have been done. In 2000 a biological phosphorus removal module by the EAWAG was added to the calibrated version of ASM 3 and is now serving the current requirements for modelling the enhanced biological P-removal. Only little experiences with different load situations of large-scale wastewater treatment plants were made with both new models so far. This article reports the experiences with the simulation and calibration of the biological parameters using ASM 3 and the EAWAG BioP Module. Three different large-scale wastewater treatment plants in Germany with different treatment systems will be discussed (Koblenz: pre-denitrification; Hildesheim: simultaneous denitrification with EBPR; Duderstadt: intermediate denitrification with EBPR). Informations regarding the choice of kinetic and stoichiometric parameters will be given.


1995 ◽  
Vol 31 (2) ◽  
pp. 13-23 ◽  
Author(s):  
M. Henze ◽  
W. Gujer ◽  
T. Mino ◽  
T. Matsuo ◽  
M. C. Wentzel ◽  
...  

The characterization of wastewater and biomass in relation to the Activated Sludge Model No. 2 is described. A new fraction of organic fermentable matter is needed. Phosphate accumulating organisms and their structural compounds polyphosphate and polyhydroxyalkanoate have to be included in the biomass characterization. There is still a need for development of analytical methods for characterization of the various components.


1995 ◽  
Vol 31 (2) ◽  
pp. 1-11 ◽  
Author(s):  
W. Gujer ◽  
M. Henze ◽  
T. Mino ◽  
T. Matsuo ◽  
M. C. Wentzel ◽  
...  

The Activated Sludge Model No. 2 is introduced as a further development of Activated Sludge Model No. 1. Model No. 2 introduces phosphorus accumulating organisms (PAO) and allows us to simulate the behaviour of biological nutrient removal activated sludge systems. Typical wastewater composition and a set of stoichiometric and kinetic parameters are provided in order to make reasonable predictions. The model has not yet been calibrated or verified in any full-scale application. This report contains a simplified version of the full Model No. 2; the full model is published in the final report of the Task Group (Henze et al., 1994).


Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3358 ◽  
Author(s):  
Daxue Li ◽  
Hailing Xi

A large amount of phosphorus was found in the extracellular polymeric substances (EPS) of activated sludge used in enhanced biological phosphorus removal (EBPR), so the role of EPS and extracellular phosphorus in EBPR should not be neglected. The composition and properties of tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were significantly different, and it was necessary to study the adsorption performance of EPS through the fractionating of activated sludge into LB-EPS, TB-EPS and microbial cells. In this study, the adsorption performance of LB-EPS and TB-EPS for phosphate was explored by extracting LB-EPS and TB-EPS via sonication and cation exchange resin (CER), respectively. The results indicated that the sonication-CER method was an efficient and reliable extraction method for EPS with a synergistic effect. The performance of EPS in the adsorption/complexing of phosphate was excellent because of its abundant functional groups. Specifically, the type and content of metal elements and functional groups in TB-EPS were much greater than those in LB-EPS, which led to the key role of TB-EPS in the adsorption/complexing of phosphate. Finally, a metabolic model for EBPR with consideration of the adsorption performance of LB-EPS and TB-EPS was proposed.


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