In vitro coronal protein signatures and biological impact of silver nanoparticles synthesized with different natural polymers as capping agents

Biopolymer-capped particles, sodium alginate- gelatin- and reconstituted silk fibroin-capped nanosilver (AgNPs) were synthesized with an intention to study, simultaneously, their in vitro and in vivo haemocompatibility, one of the major...

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Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

Nanomaterials ◽

10.3390/nano10020390 ◽

2020 ◽

Vol 10 (2) ◽

pp. 390 ◽

Cited By ~ 17

Author(s):

Flavia Resende Diniz ◽

Romerito Cesar A. P. Maia ◽

Lucas Rannier Andrade ◽

Luciana Nalone Andrade ◽

Marco Vinicius Chaud ◽

...

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Sodium Alginate ◽

Time Course ◽

In Vivo Studies ◽

Natural Polymers ◽

Cutaneous Lesions ◽

Cutaneous Wounds ◽

Healing Tissue

Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430–450 nm in the ultraviolet-visible (UV–Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT–IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm−1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.

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3D Bioprinting of Cardiovascular Tissues for In Vivo and In Vitro Applications Using Hybrid Hydrogels Containing Silk Fibroin: State of the Art and Challenges

Current Tissue Microenvironment Reports ◽

10.1007/s43152-020-00026-5 ◽

2020 ◽

Vol 1 (4) ◽

pp. 261-276

Author(s):

Laura Vettori ◽

Poonam Sharma ◽

Jelena Rnjak-Kovacina ◽

Carmine Gentile

Keyword(s):

Silk Fibroin ◽

Mechanical Stability ◽

Biological Properties ◽

Cardiac Cells ◽

3D Bioprinting ◽

Natural Polymers ◽

Cardiovascular Tissues ◽

Hybrid Hydrogels

Abstract Purpose of Review 3D bioprinting of cardiovascular tissues for in vitro and in vivo applications is currently investigated as a potential solution to better mimic the microenvironment typical of the human heart. However, optimal cell viability and tissue vascularization remain two of the main challenges in this regard. Silk fibroin (SF) as a natural biomaterial with unique features supports cell survival and tissue vascularization. This review aims to evaluate the potential of hydrogels containing SF in 3D bioprinting of cardiac tissue that better recapitulate the native cardiac microenvironment. Recent Findings SF hydrogels spontaneously develop nanocrystals, which limit their use for 3D bioprinting applications. Nevertheless, the printability of SF is improved in hybrid hydrogels by mixing it with other natural polymers (such as alginate and gelatin). This is achieved by adding SF with other polymers or by crosslinking it by peroxidase catalysis (i.e., with alginate). Compared to only SF-based hydrogels, hybrid hydrogels provide a durable bioprinted construct with improved mechanical stability and biological properties. To date, studies using cardiac cells in bioprinted SF constructs are yet to be performed. Summary Mixing SF with other polymers in bioprinted hybrid hydrogels improves the printability and durability of 3D bioprinted tissues. Studies using these hydrogels with cardiac cells will be required to evaluate the biocompatibility of SF hybrid hydrogels and to establish their potential use for cardiovascular applications.

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Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: In Vitro and In Vivo

SSRN Electronic Journal ◽

10.2139/ssrn.3600190 ◽

2020 ◽

Author(s):

Wenhao Zhou ◽

Teng Zhang ◽

Jianglong Yan ◽

QiYao Li ◽

Panpan Xiong ◽

...

Keyword(s):

Silk Fibroin ◽

Orthopedic Infection

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Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

Current Medicinal Chemistry ◽

10.2174/0929867325666180914104633 ◽

2020 ◽

Vol 27 (10) ◽

pp. 1634-1646 ◽

Cited By ~ 1

Author(s):

Huey-Shan Hung ◽

Shan-hui Hsu

Keyword(s):

Tissue Engineering ◽

Vascular Tissue ◽

Thrombus Formation ◽

Vascular Grafts ◽

Vascular Tissue Engineering ◽

Great Success ◽

Natural Polymers ◽

Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

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Silver Nanoparticles and Their Antibacterial Applications

International Journal of Molecular Sciences ◽

10.3390/ijms22137202 ◽

2021 ◽

Vol 22 (13) ◽

pp. 7202

Author(s):

Tamara Bruna ◽

Francisca Maldonado-Bravo ◽

Paul Jara ◽

Nelson Caro

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Agents ◽

Multidrug Resistant ◽

Secondary Effects ◽

Cytotoxic Effects ◽

Factors Affecting ◽

Gram Positive Bacteria ◽

Resistant Strains

Silver nanoparticles (AgNPs) have been imposed as an excellent antimicrobial agent being able to combat bacteria in vitro and in vivo causing infections. The antibacterial capacity of AgNPs covers Gram-negative and Gram-positive bacteria, including multidrug resistant strains. AgNPs exhibit multiple and simultaneous mechanisms of action and in combination with antibacterial agents as organic compounds or antibiotics it has shown synergistic effect against pathogens bacteria such as Escherichia coli and Staphylococcus aureus. The characteristics of silver nanoparticles make them suitable for their application in medical and healthcare products where they may treat infections or prevent them efficiently. With the urgent need for new efficient antibacterial agents, this review aims to establish factors affecting antibacterial and cytotoxic effects of silver nanoparticles, as well as to expose the advantages of using AgNPs as new antibacterial agents in combination with antibiotic, which will reduce the dosage needed and prevent secondary effects associated to both.

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Curcumin-incorporated crosslinked sodium alginate-g-poly (N-isopropyl acrylamide) thermo-responsive hydrogel as an in-situ forming injectable dressing for wound healing: in vitro characterization and in vivo evaluation

Carbohydrate Polymers ◽

10.1016/j.carbpol.2021.118434 ◽

2021 ◽

pp. 118434

Author(s):

Mohammad Zakerikhoob ◽

Sahar Abbasi ◽

Gholamhossein Yousefi ◽

Maral Mokhtari ◽

Mohammad Sadegh Noorbakhsh

Keyword(s):

Wound Healing ◽

Sodium Alginate ◽

In Vivo Evaluation ◽

In Situ Forming ◽

In Vitro Characterization ◽

Isopropyl Acrylamide

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Biogenic synthesis of silver nanoparticles using Piper betle aqueous extract and evaluation of its anti-quorum sensing and antibiofilm potential against uropathogens with cytotoxic effects: an in vitro and in vivo approach

Environmental Science and Pollution Research ◽

10.1007/s11356-017-1049-0 ◽

2017 ◽

Vol 25 (11) ◽

pp. 10538-10554 ◽

Cited By ~ 19

Author(s):

Ramanathan Srinivasan ◽

Loganathan Vigneshwari ◽

Tamilselvam Rajavel ◽

Ravindran Durgadevi ◽

Arunachalam Kannappan ◽

...

Keyword(s):

Silver Nanoparticles ◽

Quorum Sensing ◽

Aqueous Extract ◽

Piper Betle ◽

Biogenic Synthesis ◽

Cytotoxic Effects

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Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

10.21203/rs.3.rs-38854/v3 ◽

2020 ◽

Author(s):

Afshin Fathi ◽

Mehdi Khanmohammadi ◽

Arash Goodarzi ◽

Lale Foroutani ◽

Zahra Taherian Mobarakeh ◽

...

Keyword(s):

Wound Healing ◽

Polyvinyl Alcohol ◽

Electrospun Fiber ◽

Biochemical Properties ◽

Skin Substitute ◽

Natural Polymers ◽

Silk Fibers ◽

Wound Model

Abstract Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

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