Tissue-specific oxidative stress and element distribution after oral exposure to titanium dioxide nanoparticles in rats

Health hazards of titanium dioxide nanoparticles (TiO2-NPs) have raised severe concerns because of the paucity of information regarding the toxic effects among the population. In the present research, the in vitro and in vivo cytotoxic potential of TiO2-NPs were evaluated using flow cytometric techniques. Further, in vitro and in vivo genotoxic endpoints were estimated by means of comet, micronucleus (MN), and chromosomal aberration (CA) assays. In vitro analysis was performed at the concentration range of 10–100 µg/mL using murine RAW 264.7 cells. In vivo experiments were conducted on Albino mice (M/F) by exposing them to 200 and 500 mg/kg TiO2-NPs for 90 days. Decreased percentage of cell viability with higher doses of TiO2-NPs was evident in both in vitro and in vivo flow cytometric analysis. Further, an impaired cell cycle (G0/G1, S, and G2/M) was reflected in the present investigation following the exposure to TiO2-NPs. Increased comet scores such as tail length, % DNA in tail, tail moment, and olive moment were also observed with the higher doses of TiO2-NPs in vitro and in vivo comet assays. Finally, the in vivo MN and CA assays revealed the formation of MN and chromosomal breakage following the exposure to TiO2-NPs.

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Gender difference in hepatic toxicity of titanium dioxide nanoparticles after subchronic oral exposure in Sprague‐Dawley rats

Journal of Applied Toxicology ◽

10.1002/jat.3769 ◽

2019 ◽

Vol 39 (5) ◽

pp. 807-819 ◽

Cited By ~ 10

Author(s):

Zhangjian Chen ◽

Di Zhou ◽

Shupei Zhou ◽

Guang Jia

Keyword(s):

Titanium Dioxide ◽

Gender Difference ◽

Titanium Dioxide Nanoparticles ◽

Oral Exposure ◽

Hepatic Toxicity ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells

Toxicology Letters ◽

10.1016/j.toxlet.2008.06.869 ◽

2008 ◽

Vol 180 (3) ◽

pp. 222-229 ◽

Cited By ~ 374

Author(s):

Eun-Jung Park ◽

Jongheop Yi ◽

Kyu-Hyuck Chung ◽

Doug-Young Ryu ◽

Jinhee Choi ◽

...

Keyword(s):

Oxidative Stress ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles

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Titanium dioxide nanoparticles promote oxidative stress, autophagy and reduce NLRP3 in primary rat astrocytes

Chemico-Biological Interactions ◽

10.1016/j.cbi.2020.108966 ◽

2020 ◽

Vol 317 ◽

pp. 108966 ◽

Cited By ~ 5

Author(s):

José Antonio Pérez-Arizti ◽

José Luis Ventura-Gallegos ◽

Roberto Erasmo Galván Juárez ◽

María del Pilar Ramos-Godinez ◽

Zaira Colín-Val ◽

...

Keyword(s):

Oxidative Stress ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Rat Astrocytes

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Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts

Molecules ◽

10.3390/molecules23081955 ◽

2018 ◽

Vol 23 (8) ◽

pp. 1955 ◽

Cited By ~ 13

Author(s):

Elizabeth Huerta-García ◽

Iván Zepeda-Quiroz ◽

Helen Sánchez-Barrera ◽

Zaira Colín-Val ◽

Ernesto Alfaro-Moreno ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Cycle ◽

Cell Proliferation ◽

Cell Death ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Membrane Integrity ◽

The Body ◽

Potential Health ◽

Tio2 Nps

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry and daily life. TiO2 NPs can penetrate into the body, translocate from the lungs into the circulation and come into contact with cardiac cells. In this work, we evaluated the toxicity of TiO2 NPs on H9c2 rat cardiomyoblasts. Internalization of TiO2 NPs and their effect on cell proliferation, viability, oxidative stress and cell death were assessed, as well as cell cycle alterations. Cellular uptake of TiO2 NPs reduced metabolic activity and cell proliferation and increased oxidative stress by 19-fold measured as H2DCFDA oxidation. TiO2 NPs disrupted the plasmatic membrane integrity and decreased the mitochondrial membrane potential. These cytotoxic effects were related with changes in the distribution of cell cycle phases resulting in necrotic death and autophagy. These findings suggest that TiO2 NPs exposure represents a potential health risk, particularly in the development of cardiovascular diseases via oxidative stress and cell death.

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