Bioresponsive Microlasers with Tunable Lasing Wavelength

Nanoscale ◽  
2021 ◽  
Author(s):  
Zhiyi Yuan ◽  
Xiaotian Tan ◽  
Xuerui Gong ◽  
Chaoyang Gong ◽  
Xin Cheng ◽  
...  
Keyword(s):  
Living Cells ◽  
Lasing Wavelength ◽  
Strong Intensity ◽  
Recent Advances

Lasing particles are emerging tools for amplifying light-matter interactions at the biointerface by exploiting its strong intensity and miniaturized size. Recent advances in implementing laser particles into living cells and...

scholarly journals Lessons from Photoreceptors: Turning Off G-Protein Signaling in Living Cells

Physiology ◽  
2010 ◽  
Vol 25 (2) ◽  
pp. 72-84 ◽  
Author(s):  
Marie E. Burns ◽  
Edward N. Pugh
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Signaling System ◽  
Signaling Systems ◽  
Recent Advances ◽  
Retinal Rods

Phototransduction in retinal rods is one of the most extensively studied G-protein signaling systems. In recent years, our understanding of the biochemical steps that regulate the deactivation of the rod's response to light has greatly improved. Here, we summarize recent advances and highlight some of the remaining puzzles in this model signaling system.

scholarly journals RNA interactomics: recent advances and remaining challenges

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1824 ◽  
Author(s):  
Brigitte Schönberger ◽  
Christoph Schaal ◽  
Richard Schäfer ◽  
Björn Voß
Keyword(s):  
High Throughput ◽  
Rna Structure ◽  
Living Cells ◽  
Structure And Function ◽  
Cellular Processes ◽  
Recent Advances ◽  
Regulatory Systems ◽  
Rna Duplexes ◽  
And Function

Tight regulation of cellular processes is key to the development of complex organisms but also vital for simpler ones. During evolution, different regulatory systems have emerged, among them RNA-based regulation that is carried out mainly by intramolecular and intermolecular RNA–RNA interactions. However, methods for the transcriptome-wide detection of these interactions were long unavailable. Recently, three publications described high-throughput methods to directly detect RNA duplexes in living cells. This promises to enable in-depth studies of RNA-based regulation and will narrow the gaps in our understanding of RNA structure and function. In this review, we highlight the benefits of these methods and their commonalities and differences and, in particular, point to methodological shortcomings that hamper their wider application. We conclude by presenting ideas for how to overcome these problems and commenting on the prospects we see in this area of research.

scholarly journals Molecular Beacons: Powerful Tools for Imaging RNA in Living Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-15 ◽  
Author(s):  
Ricardo Monroy-Contreras ◽  
Luis Vaca
Keyword(s):  
Real Time ◽  
Imaging Techniques ◽  
Spatial Separation ◽  
Living Cells ◽  
Molecular Beacons ◽  
Cell Physiology ◽  
Complementary Sequence ◽  
Stem Loop ◽  
Recent Advances

Recent advances in RNA functional studies highlights the pivotal role of these molecules in cell physiology. Diverse methods have been implemented to measure the expression levels of various RNA species, using either purified RNA or fixed cells. Despite the fact that fixed cells offer the possibility to observe the spatial distribution of RNA, assays with capability to real-time monitoring RNA transport into living cells are needed to further understand the role of RNA dynamics in cellular functions. Molecular beacons (MBs) are stem-loop hairpin-structured oligonucleotides equipped with a fluorescence quencher at one end and a fluorescent dye (also called reporter or fluorophore) at the opposite end. This structure permits that MB in the absence of their target complementary sequence do not fluoresce. Upon binding to targets, MBs emit fluorescence, due to the spatial separation of the quencher and the reporter. Molecular beacons are promising probes for the development of RNA imaging techniques; nevertheless much work remains to be done in order to obtain a robust technology for imaging various RNA molecules together in real time and in living cells. The present work concentrates on the different requirements needed to use successfully MB for cellular studies, summarizing recent advances in this area.

scholarly journals Self-adaptive Biosystems Through Tunable Genetic Parts and Circuits

2020 ◽  
Author(s):  
Vittorio Bartoli ◽  
Mario di Bernardo ◽  
Thomas E. Gorochowski
Keyword(s):  
Synthetic Biology ◽  
Biological Systems ◽  
Living Cells ◽  
Complex Environments ◽  
Control Engineering ◽  
Physiological Conditions ◽  
Recent Advances ◽  
The Face ◽  
The Creation ◽  
Self Adaptive

Biological systems often need to operate in complex environments where conditions can rapidly change. This is possible due to their inherent ability to sense changes and adapt by adjusting their behavior in response. Here, we detail recent advances in the creation of synthetic genetic parts and circuits whose behaviors can be dynamically tuned through a variety of intra- and extra-cellular signals. We show how this capability lays the foundation for implementing control engineering schemes in living cells and allows for the creation of biological systems that are able to self-adapt, ensuring their functionality is maintained in the face of varying environmental and physiological conditions. We end by discussing some of the broader implications of this technology for the safe deployment of synthetic biology.

SIGNALING NETWORKS IN LIVING CELLS

2005 ◽  
Vol 45 (1) ◽  
pp. 587-603 ◽  
Author(s):  
Michael A. White ◽  
Richard G.W. Anderson
Keyword(s):  
Cell Signaling ◽  
Functional Organization ◽  
Molecular Ecology ◽  
Living Cells ◽  
Signaling Networks ◽  
Important Goal ◽  
Recent Advances ◽  
Cellular Machinery

Recent advances in cell signaling research suggest that multiple sets of signal transducing molecules are preorganized and sequestered in distinct compartments within the cell. These compartments are assembled and maintained by specific cellular machinery. The molecular ecology within a compartment creates an environment that favors the efficient and accurate integration of signaling information arriving from humoral, mechanical, and nutritional sources. The functional organization of these compartments suggests they are the location of signaling networks that naturally organize into hierarchical interconnected sets of molecules through their participation in different classes of interacting units. An important goal is to determine the contribution of the compartment to the function of these networks in living cells.

Recent advances in the synthesis of functional materials by engineered and recombinant living cells

Soft Matter ◽  
2017 ◽  
Vol 13 (38) ◽  
pp. 6672-6677 ◽  
Author(s):  
Malte Winnacker
Keyword(s):  
Synthetic Biology ◽  
Materials Science ◽  
Functional Materials ◽  
Living Cells ◽  
Recent Advances

Recent strategies for the synthesis of functional materials by living cells are highlighted, elucidating the potential of the interface of materials science and synthetic biology.

Advances in living cell-based anticancer therapeutics

2020 ◽  
Vol 8 (9) ◽  
pp. 2344-2365 ◽  
Author(s):  
He Dong ◽  
Xiao Xu ◽  
Leikun Wang ◽  
Ran Mo
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
Anticancer Drug ◽  
Living Cell ◽  
Drug Carriers ◽  
Living Cells ◽  
Anticancer Drug Delivery ◽  
Recent Advances ◽  
Anticancer Therapeutics

This review summarizes recent advances in the applications of living cells as drug carriers or active drugs for anticancer drug delivery and cancer therapy.

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