scholarly journals Pro-organic radical contrast agents (“pro-ORCAs”) for real-time MRI of pro-drug activation in biological systems

2020 ◽  
Vol 11 (29) ◽  
pp. 4768-4779 ◽  
Author(s):  
Hung V.-T. Nguyen ◽  
Alexandre Detappe ◽  
Peter Harvey ◽  
Nolan Gallagher ◽  
Clelia Mathieu ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Ring Opening ◽  
Organic Radical ◽  
Real Time Monitoring ◽  
Prodrug Activation ◽  
Metathesis Polymerization ◽  
Drug Activation

Pro-organic radical contrast agents (pro-ORCAs) that enable real-time monitoring of doxorubicin prodrug activation in vitro and in vivo are prepared through brush-first ring-opening metathesis polymerization (ROMP).

Facile solvothermal synthesis of ultrathin spinel ZnMn2O4 nanospheres: An efficient electrocatalyst for in vivo and in vitro real time monitoring of H2O2

2021 ◽  
Vol 900 ◽  
pp. 115674
Author(s):  
Muthaiah Annalakshmi ◽  
Sakthivel Kumaravel ◽  
T.S.T. Balamurugan ◽  
Shen-Ming Chen ◽  
Ju-Liang He
Keyword(s):  
Real Time ◽  
Solvothermal Synthesis ◽  
Real Time Monitoring

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

Intracellular GSH-responsive camptothecin delivery systems

2019 ◽  
Vol 43 (47) ◽  
pp. 18673-18684 ◽  
Author(s):  
Dan Zhang ◽  
Le Li ◽  
Xiaohui Ji ◽  
Yanhong Gao
Keyword(s):  
Real Time ◽  
Delivery Systems ◽  
Real Time Monitoring

Smart GSH-responsive camptothecin delivery systems for treatment of tumors and real-time monitoring in vivo and in vitro were described.

Real-time monitoring of anticancer drug release in vitro and in vivo on titania nanoparticles triggered by external glutathione

Talanta ◽  
2012 ◽  
Vol 88 ◽  
pp. 631-637 ◽  
Author(s):  
Mira Kim ◽  
Ji Hye Seo ◽  
Won Il Jeon ◽  
Mi-Yeon Kim ◽  
Keunchang Cho ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Anticancer Drug ◽  
Titania Nanoparticles ◽  
Real Time Monitoring ◽  
Release In Vitro

scholarly journals 181. Real-Time Monitoring of Transcription Factor Activity Using In Vitro and In Vivo Models of Cholestasis

2015 ◽  
Vol 23 ◽  
pp. S72
Author(s):  
Juliette M.K.M. Delhove ◽  
Dany Perocheau ◽  
Suzanne M.K. Buckley ◽  
Rajvinder Karda ◽  
Simon N. Waddington ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Real Time ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Real Time Monitoring ◽  
In Vivo Models

Abstract 5960: Implementation of Noninvasive Subharmonic Pressure Estimation on a Commercial Ultrasound Scanner

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Lauren M Leodore ◽  
Corina Leung ◽  
Laurent Pelissier ◽  
Flemming Forsberg
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Pressure Range ◽  
Linear Regression Analysis ◽  
Heart Association ◽  
Center Frequency ◽  
Single Element ◽  
Pressure Estimation

This project aims to monitor and quantify intra-cardiac pressures via contrast-enhanced subharmonic imaging. We have proposed subharmonic aided pressure estimation (SHAPE; U.S. Patent 6,302,845) utilizing microbubble-based contrast agent signals for the noninvasive estimation of hydrostatic blood pressures in the heart cavities and in this study implemented real-time SHAPE on a commercial US scanner. An experimental pulse-echo system for SHAPE was constructed based on two single element transducers assembled confocally at a 60° angle to each other. A transducer with a bandwidth of 38% and a center frequency of 2.2 MHz (Staveley, East Hartford, CT) was used as the transmitter and a second transducer with a bandwidth of 86% and a center frequency of 3.6 MHz (Etalon Inc., Lebanon, IN) was the receiver. Changes in first, second, and subharmonic amplitudes of 6 different US contrast agents were measured in vitro at hydrostatic pressures from 0 –186 mmHg, acoustic pressures from 0.35– 0.60 MPa and frequencies of 2.5– 6.6 MHz. The optimal parameters for SHAPE were determined using linear regression analysis and implemented on a Sonix RP scanner (Ultrasonix Medical Corp, Richmond, Canada). The real-time implementation of SHAPE was tested in vitro. Over the pressure range studied the 1st and 2nd harmonic amplitudes reduced ~2 dB for all US contrast agents. Over the same pressure range, the subharmonic amplitudes decreased by 10 –14 dB and excellent linear regressions were achieved with the hydrostatic pressure variations (r 2 >0.98, p < 0.001). The optimal sensitivity for SHAPE was achieved at a transmit frequency of 2.5 MHz (i.e., receiving at 1.25 MHz) at a 0.35 MPa acoustic pressure using Sonazoid (GE Healthcare, Oslo, Norway) which declined ~14.4 dB in vitro. A Sonix RP scanner was modified to implement SHAPE on a phased array transducer PA4 –2 with a frequency range from 1.5– 4.5 MHz. A pulse inversion technique was used and the subharmonic signals are displayed in real-time and can also be stored for off-line analysis. SHAPE offers the possibility of allowing pressure gradients in the heart to be obtained noninvasively. Future studies will include in vivo pressure measurements. Supported by AHA grant no 06554414 and NIH HL081892. This research has received full or partial funding support from the American Heart Association, AHA Great Rivers Affiliate (Delaware, Kentucky, Ohio, Pennsylvania & West Virginia).

scholarly journals Refinement of a mouse cardiovascular model: Development, application and dissemination

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 593
Author(s):  
Kirk A. Taylor ◽  
Michael Emerson
Keyword(s):  
Real Time ◽  
Light Transmission ◽  
Ex Vivo ◽  
Model Development ◽  
Cell Counting ◽  
Real Time Monitoring ◽  
Mortality Models ◽  
Typical Experiment

European and UK legislation requires all animal procedures to be conducted with consideration to reduction, refinement and replacement. In this review, 3Rs developments are discussed in the field of platelet biology and thromboembolism. Platelet research requires the use of animal models, and mice are widely used in the field. When working in vitro, conventional light transmission techniques have been scaled down allowing reduction in animal numbers. In vivo, vascular injury models are widely used and work is ongoing to develop ex vivo approaches that use fewer animals. Thromboembolic mortality models, which inflict considerable pain and suffering, have also been used widely. A published and characterised refinement of this mortality model allows real-time monitoring of radiolabelled platelets under general anaesthesia and reduces both the severity level and the numbers of mice used in a typical experiment. This technique is more sensitive than the mortality approach and has opened up new avenues of research, which would not have been feasible by using death as an end-point. To drive uptake of real-time monitoring, a more simplistic approach has been developed involving micro-sampling and cell counting. Thromboembolic mortality models should therefore be considered obsolete due to the emergence of 3Rs models with improved scientific outcomes and that can be implemented relatively easily.

scholarly journals Self-carried curcumin nanoparticles for in vitro and in vivo cancer therapy with real-time monitoring of drug release

Nanoscale ◽  
2015 ◽  
Vol 7 (32) ◽  
pp. 13503-13510 ◽  
Author(s):  
Jinfeng Zhang ◽  
Shengliang Li ◽  
Fei-Fei An ◽  
Juan Liu ◽  
Shubin Jin ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Drug Release ◽  
Real Time ◽  
Real Time Monitoring ◽  
Curcumin Nanoparticles ◽  
Major Attention ◽  
Pharmaceutical Agents

The use of different nanocarriers for delivering hydrophobic pharmaceutical agents to tumor sites has garnered major attention.

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