scholarly journals An LC-MS/MS method for protein detection based on a mass barcode and dual-target recognition strategy

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 16094-16100
Author(s):  
Duo Li ◽  
Qinxin Song ◽  
Tengfei Li ◽  
Chang Shu ◽  
Shunli Ji ◽  
...  

A mass barcode-mediated signal amplification method was developed for the determination of protein by LC-MS/MS.

1995 ◽  
Vol 43 (4) ◽  
pp. 347-352 ◽  
Author(s):  
H M Kerstens ◽  
P J Poddighe ◽  
A G Hanselaar

For amplification of in situ hybridization (ISH) signals, we describe a method using catalyzed reporter deposition (CARD). This amplification method is based on the deposition of biotinylated tyramine (BT) at the location of the DNA probe. The BT precipitate can then visualized with fluorochrome- or enzyme-labeled avidin. Both for bright-field ISH (BRISH) and for fluorescence ISH (FISH), the detection limit was highly increased. This method is especially suitable for visualization of very weak ISH signals, such as those obtained by ISH using locus-specific DNA probes. Furthermore, CARD amplification of ISH signals (CARD-ISH) is highly sensitive, rapid, flexible, and easy to implement. Successful application of CARD-ISH with locus-specific DNA probes on histological and cytological samples may improve the determination of structural chromosomal aberrations in archival material.


The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2799-2806 ◽  
Author(s):  
Erhu Xiong ◽  
Xiaoxia Yan ◽  
Xiaohua Zhang ◽  
Yanmei Li ◽  
Ruiying Yang ◽  
...  

Based on a “signal-on” sensing strategy and a three-stage cascade signal amplification method, an ultrasensitive photoelectrochemical biosensor has been developed for DNA detection.


Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.


The Analyst ◽  
2021 ◽  
Author(s):  
Xinli Liu ◽  
yanping wang ◽  
Yanfeng Gao ◽  
Yujun Song

Gas-propelled biosensors display a simple gas-based signal amplification with quantitative detection features based on the target recognition event in combination with gas propulsion. Due to the liquid–gas conversion, the gas...


2017 ◽  
Vol 100 (4) ◽  
pp. 950-961 ◽  
Author(s):  
María Pedrero ◽  
Susana Campuzano ◽  
José M Pingarrón

Abstract The determination of organic and inorganic environmental and food pollutants is a key matter of concern in analytical chemistry due to their effects as a serious threat to human health. Focusing on this issue, several methodologies involving the use of nanostructured electrochemical platforms have been recently reported in the literature. Among these methods, those employing the use of quantum dots (QDs) stand out because of features such as signal amplification, good reproducibility and selectivity, and the possibility for multiplexed detection, and because they preserve the outstanding characteristics of electrochemical methodologies with respect to simplicity, ease-of-use, and cost-effective instrumentation. This review describes recent electrochemical strategies, in which design QDs play a key role, for the determination of pollutants in food and environmental samples. The particular role of QDs in the reported methodologies, their preparation, and the electrochemical platform design, as well as the advantages that QDs provide in the analysis of target analytes, are critically discussed.


BioTechniques ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 815-818 ◽  
Author(s):  
Johanna R. Mora ◽  
Tamara L. Zielinski ◽  
Bryce P. Nelson ◽  
Robert C. Getts

The Analyst ◽  
2017 ◽  
Vol 142 (22) ◽  
pp. 4215-4220 ◽  
Author(s):  
Dazhi Yao ◽  
Wenqi Zhao ◽  
Limin Zhang ◽  
Yang Tian

Developing a sensitive and accurate method for Furin activity is still the bottleneck for understanding the role played by Furin in cell-surface systems and even in Alzheimer's disease.


2016 ◽  
Vol 8 (16) ◽  
pp. 3366-3372 ◽  
Author(s):  
Hai-Yan Song ◽  
Tian-Fang Kang ◽  
Na-Na Li ◽  
Li-Ping Lu ◽  
Shui-Yuan Cheng

In this paper, a novel aptamer sensor for kanamycin was prepared by using a complementary DNA (cDNA) strand of kanamycin aptamer as sensor and horseradish peroxidase (HRP) as a biocatalyst for signal amplification.


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