scholarly journals High-efficiency dynamic sensing of biothiols in cancer cells with a fluorescent β-cyclodextrin supramolecular assembly

2020 ◽  
Vol 11 (18) ◽  
pp. 4791-4800 ◽  
Author(s):  
Zhixue Liu ◽  
Weilei Zhou ◽  
Jingjing Li ◽  
Haoyang Zhang ◽  
Xianyin Dai ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Real Time ◽  
High Efficiency ◽  
Supramolecular Assembly ◽  
Real Time Monitoring

A unique fluorescent supramolecular assembly was constructed from coumarin-modified β-cyclodextrin and an adamantane-modified cyclic arginine–glycine–aspartate peptide for high-efficiency real-time monitoring of biothiols in cancer cells.

scholarly journals Detection of Clinical Mesenchymal Cancer Cells from Bladder Wash Urine for Real-Time Detection and Prognosis

Cancers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1274 ◽  
Author(s):  
Bee Luan Khoo ◽  
Charlotte Bouquerel ◽  
Pradeep Durai ◽  
Sarannya Anil ◽  
Benjamin Goh ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
High Efficiency ◽  
Stream Line ◽  
Clinical Samples ◽  
High Recurrence Rate ◽  
Mesenchymal Transition ◽  
Non Invasive ◽  
Real Time Detection

Bladder cancer (BC) is a disease that requires lifelong surveillance due to its high recurrence rate. An efficient method for the non-invasive rapid monitoring of patient prognosis and downstream phenotype characterization is warranted. Here, we develop an integrated procedure to detect aggressive mesenchymal exfoliated bladder cancer cells (EBCCs) from patients in a label-free manner. Using a combination of filtration and inertial focusing principles, the procedure allowed the focusing of EBCCs in a single stream-line for high-throughput separation from other urine components such as large squamous cells and blood cells using a microfluidic sorting device. Characterization of enriched cells can be completed within hours, suggesting a potential utility for real-time detection. We also demonstrate high efficiency of cancer cell recovery (93.3 ± 4.8%) and specific retrieval of various epithelial to mesenchymal transition (EMT) phenotype cell fractions from respective outlets of the microfluidic device. EMT is closely associated with metastasis, drug resistance and tumor-initiating potential. This procedure is validated with clinical samples, and further demonstrate the efficacy of bladder wash procedure to reduce EBCCs counts over time. Overall, the uniqueness of a rapid and non-invasive method permitting the separation of different EMT phenotypes shows high potential for clinical utility. We expect this approach will better facilitate the routine screening procedure in BC and greatly enhance personalized treatment.

scholarly journals Monitoring the dynamics of hemeoxygenase-1 activation in head and neck cancer cells in real-time using plasmonically enhanced Raman spectroscopy

2019 ◽  
Vol 10 (18) ◽  
pp. 4876-4882 ◽  
Author(s):  
Sajanlal R. Panikkanvalappil ◽  
Chakravarthy Garlapati ◽  
Nasrin Hooshmand ◽  
Ritu Aneja ◽  
Mostafa A. El-Sayed
Keyword(s):  
Raman Spectroscopy ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Real Time ◽  
Neck Cancer ◽  
Mammalian Cells ◽  
Real Time Monitoring

Real-time monitoring of the dynamics of pharmacologically generated HO-1 in mammalian cells by using plasmonically enhanced Raman spectroscopy (PERS).

A fluorescence off–on reporter for real time monitoring of gemcitabine delivery to the cancer cells

2013 ◽  
Vol 49 (64) ◽  
pp. 7141 ◽  
Author(s):  
Sankarprasad Bhuniya ◽  
Min Hee Lee ◽  
Hyun Mi Jeon ◽  
Ji Hye Han ◽  
Jae Hong Lee ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Real Time ◽  
Real Time Monitoring

Real-time monitoring approach: Assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix

2008 ◽  
Vol 23 (9) ◽  
pp. 1390-1396 ◽  
Author(s):  
Yu Chen ◽  
Juntao Zhang ◽  
Yanping Wang ◽  
Li Zhang ◽  
Reboud Julien ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cancer Cells ◽  
Real Time ◽  
Real Time Monitoring

scholarly journals High Efficiency Recombinant Protein Purification Using mCherry and YFP Nanobody Affinity Matrices

2021 ◽  
Author(s):  
Anh T.Q. Cong ◽  
Taylor L. Witter ◽  
Matthew J. Schellenberg
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Protein Purification ◽  
Real Time ◽  
High Efficiency ◽  
Expression System ◽  
Expression Systems ◽  
Protein Fusion ◽  
Real Time Monitoring ◽  
Mammalian Cell Lines

Mammalian cell lines are important expression systems for large proteins and protein complexes, particularly when the acquisition of post-translational modifications in the proteins native environment is desired. However, low or variable transfection efficiencies are challenges that must be overcome to use such an expression system. Expression of recombinant proteins as a fluorescent protein fusion enables real-time monitoring of protein expression, and also provides an affinity handle for one-step protein purification using a suitable affinity reagent. Here we describe a panel of anti-GFP and anti-mCherry nanobody affinity matrices and their efficacy for purification of GFP/YFP or mCherry fusion proteins. We define the molecular basis by which they bind their target protein using X-ray crystallography. From these analyses we define an optimal pair of nanobodies for purification of recombinant protein tagged with GFP/YFP or mCherry, and demonstrate these nanobody-sepharose supports are stable to many rounds of cleaning and extended incubation in denaturing conditions. Finally, we demonstrate the utility of the mCherry-tag system by using it to purify recombinant human Topoisomerase 2α expressed in HEK293F cells. The mCherry-tag and GFP/YFP-tag expression systems can be utilized for recombinant protein expression individually or in tandem for mammalian protein expression systems where real-time monitoring of protein expression levels and a high-efficiency purification step is needed.

Telescope performance real-time monitoring based on machine learning

2020 ◽  
Vol 500 (1) ◽  
pp. 388-396
Author(s):  
Tian Z Hu ◽  
Yong Zhang ◽  
Xiang Q Cui ◽  
Qing Y Zhang ◽  
Ye P Li ◽  
...  
Keyword(s):  
Machine Learning ◽  
Real Time ◽  
System Performance ◽  
High Performance ◽  
High Efficiency ◽  
Peak Performance ◽  
Source Image ◽  
Detection Accuracy ◽  
Real Time Monitoring ◽  
Active Optics

ABSTRACT In astronomy, the demand for high-resolution imaging and high-efficiency observation requires telescopes that are maintained at peak performance. To improve telescope performance, it is useful to conduct real-time monitoring of the telescope status and detailed recordings of the operational data of the telescope. In this paper, we provide a method based on machine learning to monitor the telescope performance in real-time. First, we use picture features and the random forest algorithm to select normal pictures captured by the acquisition camera or science camera. Next, we cut out the source image of the picture and use convolutional neural networks to recognize star shapes. Finally, we monitor the telescope performance based on the relationship between the source image shape and telescope performance. Through this method, we achieve high-performance real-time monitoring with the Large Sky Area Multi-Object Fibre Spectroscopic Telescope, including guiding system performance, focal surface defocus, submirror performance, and active optics system performance. The ultimate performance detection accuracy can reach up to 96.7 per cent.

scholarly journals Real-time monitoring and control for high-efficiency autonomous laser fabrication of silicon nanoparticle colloids

2021 ◽  
Vol 114 (1-2) ◽  
pp. 291-304
Author(s):  
Brian Freeland ◽  
Ronan McCann ◽  
Paul O’Neill ◽  
Sithara Sreenilayam ◽  
Manuel Tiefenthaler ◽  
...  
Keyword(s):  
Real Time ◽  
High Efficiency ◽  
Monitoring And Control ◽  
Real Time Monitoring ◽  
Silicon Nanoparticle ◽  
Laser Fabrication ◽  
And Control

Precise imaging of mitochondria in cancer cells by real-time monitoring of nitroreductase activity with a targetable and activatable fluorescent probe

2020 ◽  
Vol 56 (56) ◽  
pp. 7761-7764 ◽  
Author(s):  
Ning Zhu ◽  
Ge Xu ◽  
Rongchen Wang ◽  
Tianli Zhu ◽  
Jiahui Tan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Real Time ◽  
Fluorescent Probe ◽  
Real Time Monitoring ◽  
Ratiometric Fluorescence ◽  
Nir Emission ◽  
Emission Light ◽  
Nitroreductase Activity

A probe with a targetable feature and ratiometric fluorescence as well as NIR emission light-up response to nitroreductase is presented for the precise imaging of mitochondria in cancer cells by real-time monitoring of nitroreductase activity.

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