Amorphous colloidal photonic crystals assembled by mesoporous silica particles for thin layer chromatography with high separation efficiency and colorimetric recognition

2020 ◽  
Vol 8 (48) ◽  
pp. 17202-17210
Author(s):  
Xin Zhang ◽  
Yumei Ran ◽  
Qianqian Fu ◽  
Jianping Ge
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystals ◽  
Mesoporous Silica ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Separation Efficiency ◽  
Colloidal Photonic Crystals ◽  
Crystal Film ◽  
Layer Chromatography ◽  
High Separation

A mesoporous SiO2 amorphous photonic crystal film was used to develop new thin-layer chromatography with high separation efficiency and simplified recognition.

High Performance Thin Layer Chromatography: A Mini Review

2016 ◽  
Vol 2 (4) ◽  
pp. 219-226
Author(s):  
S. V. Saibaba ◽  
P. Shanmuga Pandiyan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Separation Efficiency ◽  
Analytical Tool ◽  
Combinatorial Approach ◽  
Herbal Drug ◽  
Biomedical Analysis ◽  
Layer Chromatography

High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have made HPTLC a power analytical tool in the field of analysis. Experts are of the opinion that HPTLC future to combinatorial approach and the utilization of instrumental HPTLC toward the analysis of drug formulations, bulk drugs, and natural products will increase in the future

scholarly journals Thin-layer chromatography of L-carnitine in micellar and cyclodextrin mobile phases

2019 ◽  
Vol 58 (5) ◽  
pp. 62-69
Author(s):  
Anna O. Ustyugova ◽  
◽  
Elena G. Sumina ◽  
Varsenia Z. Uglanova ◽  
Olga N. Novozhilova ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Separation Efficiency ◽  
Sodium Dodecyl ◽  
Theoretical Plate ◽  
Process Efficiency ◽  
Chromatographic Behavior ◽  
Mobile Phases ◽  
Wide Range ◽  
Layer Chromatography

L-carnitine (lat. Levocarnitinum, eng. Levocarnitine) is a natural substance akin to B vitamins. Normalizing metabolic processes in the human body and having a wide range of therapeutic actions, L-carnitine is widely used in cardiology, neurology, gastroenterology, and sports medicine. Currently, modern analytical techniques, such as mass spectrometry, high performance liquid chromatography, radiation methods, etc., are used to analyze L-carnitine. Most of them are rather expensive and time consuming. Thin-layer chromatography (TLC), which is notable for its simplicity, feasibility of analysis, separation efficiency and low cost, has received much less use. In this regard, the purpose of this work was to reveal the analytical capabilities of water-organic, micellar and cyclodextrin mobile phases for the analysis of L-carnitine by means of TLC. Initial L-carnitine solutions with a concentration of 20 mg/ml were prepared by diluting solutions for injections. Their chromatography was carried out by ascending thin-layer chromatography on plates of various polarities (Sorbfil, Plasmachrom, and RP-18) with a fixed sorbent layer. Aqueous solutions of surfactants (cetyltrimethylammonium bromide, cetylpyridinium chloride, sodium dodecyl sulfate, Triton X-100), of cyclodextrins (-cyclodextrin, 2-hydroxypropyl--cyclodextrin), hydroxypropyl--cyclodextrin) were used as mobile phases. The zone of the main substance (L-carnitine) was identified by treating the plate with a mixture of freshly prepared 0.1N solution of potassium permanganate and 2.0 N acetic acid (the zone was colored in a light yellow color). Main features of the chromatographic behavior of L-carnitine were revealed. Based on the calculation of the number of theoretical plates and the height equivalent to one theoretical plate, it was shown that aqueous micellar and cyclodextrin mobile phases allow one to improve the chromatographic process efficiency and the shape of the chromatographic zones of L-carnitine as compared to water-organic eluents; cheaper Sorbfil plates (Russia) can be used for practical purposes. Optimal chromatographic systems and conditions for their use in the thin-layer chromatography of pharmaceuticals and food products have been selected and substantiated.

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