Rotational scan digital LAMP for accurate quantitation of nucleic acids

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Mengcheng Jiang ◽  
Peiyu Liao ◽  
Yue Sun ◽  
Xinyang Shao ◽  
Zitian Chen ◽  
...  

Droplets generated by centrifugation can be packed as a thin layer by flipping a micro-centrifugal tube with a plastic insert. RS-dLAMP applies line-scan to enable high-speed and contamination-free digital quantification of nucleic acids.

1993 ◽  
Author(s):  
David J. Litwiller ◽  
Mike Miethig ◽  
Brian C. Doody ◽  
P. Tom Jenkins

2015 ◽  
Vol 5 (5) ◽  
pp. 20150048 ◽  
Author(s):  
Qianxi Wang ◽  
Wenke Liu ◽  
A. M. Zhang ◽  
Yi Sui

A bubble initiated near a rigid boundary may be almost in contact with the boundary because of its expansion and migration to the boundary, where a thin layer of water forms between the bubble and the boundary thereafter. This phenomenon is modelled using the weakly compressible theory coupled with the boundary integral method. The wall effects are modelled using the imaging method. The numerical instabilities caused by the near contact of the bubble surface with the boundary are handled by removing a thin layer of water between them and joining the bubble surface with its image to the boundary. Our computations correlate well with experiments for both the first and second cycles of oscillation. The time history of the energy of a bubble system follows a step function, reducing rapidly and significantly because of emission of shock waves at inception of a bubble and at the end of collapse but remaining approximately constant for the rest of the time. The bubble starts being in near contact with the boundary during the first cycle of oscillation when the dimensionless stand-off distance γ = s / R m < 1, where s is the distance of the initial bubble centre from the boundary and R m is the maximum bubble radius. This leads to (i) the direct impact of a high-speed liquid jet on the boundary once it penetrates through the bubble, (ii) the direct contact of the bubble at high temperature and high pressure with the boundary, and (iii) the direct impingement of shock waves on the boundary once emitted. These phenomena have clear potential to damage the boundary, which are believed to be part of the mechanisms of cavitation damage.


1994 ◽  
Author(s):  
David J. Litwiller ◽  
Mike Miethig ◽  
Brian C. Doody ◽  
P. Tom Jenkins

The behaviour of thin layers of solid materials under drop-weight impact is studied with the aid of high-speed photographic and pressure-measuring techniques. Photographic sequences taken with a high-speed framing camera show that explosive materials suffer large-scale deformation before initiation of explosion. The sample may undergo plastic flow in bulk, show evidence of partial fusion, and even (with PETN) melt completely. There is also evidence of Munroe jetting and instability of flow of material at the anvil/layer interfaces. The flow speed of the sample during these processes is considerable and may reach 300 m/s. When ignition of the layer occurs it does so at a small number of local hot spots, following which rapid combustion develops at speeds of 200-700 m/s. Strain-gauge measurements show that the pressures attained during drop-weight impact are typically 0.5-1 GPa (5–10 kbar) and the duration of impact 300–500 μs. In the course of impact of a thin layer of granular material a sharp pressure drop may occur, frequently from several hundred MPa down to zero. With an explosive layer, ignition occurs immediately following the instant of the pressure drop. The sudden fall in pressure is due to mechanical failure of the sample, and correlation of the two experiments shows that this is the cause of the very high flow speeds attained during impact. On the basis of these results a possible mechanism of ignition is suggested.


1976 ◽  
Vol 128 (1) ◽  
pp. 101-110 ◽  
Author(s):  
U.A.Th. Brinkman ◽  
A. de Kok ◽  
G. de Vries ◽  
H.G.M. Reymer

2020 ◽  
Vol 11 (9) ◽  
pp. 5274 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Xiaoyun Jiang ◽  
Aiden Maloney-Bertelli ◽  
James A. Kuchenbecker ◽  
Utkarsh Sharma ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document