scholarly journals Microfluidic organ-on-chip system for multi-analyte monitoring of metabolites in 3D cell cultures

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Johannes Dornhof ◽  
Jochen Kieninger ◽  
Harshini Muralidharan ◽  
Jochen Maurer ◽  
Gerald A. Urban ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Cultures ◽  
Metabolic Monitoring ◽  
3D Cell Cultures ◽  
Single Cancer ◽  
Organoid Cultures ◽  
On Chip

An organ-on-chip platform equipped with microsensors for long-term microfluidic cultivation and metabolic monitoring (O2, Glu, Lac) of 3D tumour organoid cultures grown from patient-derived single cancer stem cells.

scholarly journals CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

BMC Cancer ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Cristina Patru ◽  
Luciana Romao ◽  
Pascale Varlet ◽  
Laure Coulombel ◽  
Eric Raponi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Neuronal Tumors

scholarly journals Targeting AMP ‐activated kinase impacts hepatocellular cancer stem cells induced by long‐term treatment with sorafenib

2019 ◽  
Vol 13 (5) ◽  
pp. 1311-1331 ◽  
Author(s):  
Alicia Bort ◽  
Belén G. Sánchez ◽  
Pedro A. Mateos‐Gómez ◽  
Diana Vara‐Ciruelos ◽  
Nieves Rodríguez‐Henche ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Hepatocellular Cancer ◽  
Term Treatment ◽  
Long Term Treatment

Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment

2009 ◽  
Vol 219 (2) ◽  
pp. 301-313 ◽  
Author(s):  
Riccardo Di Fiore ◽  
Andrea Santulli ◽  
Rosa Drago Ferrante ◽  
Michela Giuliano ◽  
Anna De Blasio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Human Osteosarcoma

scholarly journals Long‑term model of colitis‑associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells

2020 ◽  
Vol 21 (1) ◽  
pp. 1-1
Author(s):  
Yuji Tanimura ◽  
Toshiro Fukui ◽  
Shunsuke Horitani ◽  
Yasushi Matsumoto ◽  
Sachi Miyamoto ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Tumor Spread ◽  
Term Model

scholarly journals Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sandra Valle ◽  
Sonia Alcalá ◽  
Laura Martin-Hijano ◽  
Pablo Cabezas-Sáinz ◽  
Diego Navarro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Ductal Adenocarcinoma ◽  
In Vitro System ◽  
Tumorigenic Potential ◽  
Term Enrichment ◽  
Pluripotency Gene ◽  
Pancreatic Cancer Stem Cells

Abstract Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate of approximately 7–9%. The ineffectiveness of anti-PDAC therapies is believed to be due to the existence of a subpopulation of tumor cells known as cancer stem cells (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant and metastatic capacities. Herein, we describe a 2D in vitro system for long-term enrichment of pancreatic CSCs that is amenable to biological and CSC-specific studies. By changing the carbon source from glucose to galactose in vitro, we force PDAC cells to utilize OXPHOS, resulting in enrichment of CSCs defined by increased CSC biomarker and pluripotency gene expression, greater tumorigenic potential, induced but reversible quiescence, increased OXPHOS activity, enhanced invasiveness, and upregulated immune evasion properties. This CSC enrichment method can facilitate the discovery of new CSC-specific hallmarks for future development into targets for PDAC-based therapies.

scholarly journals Effect of Delay on Selection Dynamics in Long-Term Sphere Culture of Cancer Stem Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Peng Tang ◽  
Aijun Fan ◽  
Jianquan Li ◽  
Jun Jiang ◽  
Kaifa Wang
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Time Delay ◽  
Cancer Stem Cells ◽  
Cell Populations ◽  
Delay Effect ◽  
Selection Dynamics ◽  
Sphere Culture ◽  
Theoretical Results

To quantitatively study the effect of delay on selection dynamics in long-term sphere culture of cancer stem cells (CSCs), a selection dynamic model with time delay is proposed. Theoretical results show that the ubiquitous time delay in cell proliferation may be one of the important factors to induce fluctuation, and numerical simulations indicate that the proposed selection dynamical model with time delay can provide a better fitting effect for the experiment of a long-term sphere culture of CSCs. Thus, it is valuable to consider the delay effect in the future study on the dynamics of nongenetic heterogeneity of clonal cell populations.

A lab-on-chip ultrasonic platform for real-time and nondestructive assessment of extracellular matrix stiffness

Lab on a Chip ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 778-788 ◽  
Author(s):  
Amin Zareei ◽  
Hongjie Jiang ◽  
Shirisha Chittiboyina ◽  
Jiawei Zhou ◽  
Beatriz Plaza Marin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Ultrasonic Wave ◽  
Cell Cultures ◽  
Wave Attenuation ◽  
Matrix Stiffness ◽  
Lab On Chip ◽  
3D Cell Cultures ◽  
Extracellular Matrix Stiffness ◽  
Noninvasive Assessment ◽  
On Chip

On-chip ultrasonic platform enables noninvasive assessment of ECM stiffness in 3D cell cultures, by monitoring ultrasonic wave attenuation through targeted material.

scholarly journals Nanoparticle Delivery of miR-34a Eradicates Long-term-cultured Breast Cancer Stem Cells via Targeting C22ORF28 Directly

Theranostics ◽  
2017 ◽  
Vol 7 (19) ◽  
pp. 4805-4824 ◽  
Author(s):  
Xiaoti Lin ◽  
Weiyu Chen ◽  
Fengqin Wei ◽  
Binhua P. Zhou ◽  
Mien-Chie Hung ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Breast Cancer Stem Cells ◽  
Nanoparticle Delivery
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