Ratiometric fluorescence analysis for miR-141 detection with hairpin DNA-templated silver nanoclusters

Journal of Materials Chemistry C ◽

10.1039/d1tc04488e ◽

2021 ◽

Author(s):

Meiqing Liu ◽

Ren Shen ◽

Haoran Li ◽

Yanwei Jia ◽

Pui-In Mak ◽

...

Keyword(s):

Early Diagnosis ◽

Fluorescence Analysis ◽

Silver Nanoclusters ◽

Ratiometric Fluorescence ◽

Hairpin Dna ◽

Robust Detection ◽

Diagnosis And Prognosis

MicroRNAs (miRNAs) are important biomarkers for diseases diagnosis and prognosis. Accurate and robust detection of miRNAs greatly facilitates the early diagnosis and progress assessment of certain cancers. The current miRNAs...

Download Full-text

DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations

Research ◽

10.34133/2020/1091605 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Yiting Jiang ◽

Peng Miao

Keyword(s):

Early Diagnosis ◽

Logic Gates ◽

Silver Nanoclusters ◽

Dna Nanostructures ◽

Fundamental Issue ◽

Ratiometric Fluorescence ◽

Complex Biological System ◽

Dna Nanostructure ◽

Logic Operations ◽

Selective Analysis

Multiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell structures are firstly designed with two independent nucleation sequences for AgNCs at the 5′ and 3′ ends, respectively. By introducing different miRNA inputs, separations of two AgNCs are controlled and the fluorescence property of AgNCs changes. By studying the ratiometric fluorescence responses, sensitive and selective analysis of multiple miRNAs can be achieved. The present work provides powerful tools for miRNA diagnostics and may also guide future DNA nanostructure-based logic gates.

Download Full-text

Protein biomarkers in breast cancer-derived extracellular vesicles for use in liquid biopsies

AJP Cell Physiology ◽

10.1152/ajpcell.00048.2021 ◽

2021 ◽

Author(s):

Dan Li ◽

Wenjia Lai ◽

Di Fan ◽

Qiaojun Fang

Keyword(s):

Breast Cancer ◽

Early Diagnosis ◽

Extracellular Vesicles ◽

Liquid Biopsy ◽

Breast Cancer Diagnosis ◽

Extracellular Vesicle ◽

Detection Methods ◽

Protein Markers ◽

Liquid Biopsies ◽

Diagnosis And Prognosis

Breast cancer is the most common malignant disease in women worldwide. Early diagnosis and treatment can greatly improve the management of breast cancer. Liquid biopsies are becoming convenient detection methods for diagnosing and monitoring breast cancer due to their non-invasiveness and ability to provide real-time feedback. A range of liquid biopsy markers, including circulating tumor proteins, circulating tumor cells, and circulating tumor nucleic acids, have been implemented for breast cancer diagnosis and prognosis, with each having its own advantages and limitations. Circulating extracellular vesicles are messengers of intercellular communication that are packed with information from mother cells and are found in a wide variety of bodily fluids; thus, they are emerging as ideal candidates for liquid biopsy biomarkers. In this review, we summarize extracellular vesicle protein markers that can be potentially used for the early diagnosis and prognosis of breast cancer or determining its specific subtypes.

Download Full-text