scholarly journals A rapid method for collecting and assaying gradient fractions for radioactive macromolecules (Short Communication)

1974 ◽  
Vol 137 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Leonard Rubinstein ◽  
Alan Rein

A rapid method is described for collecting gradients after centrifugation, and assaying them for acid-precipitable radioactivity. An entire gradient is collected and processed on a single glass-fibre filter disc. This method allows one easily to assay six gradients for acid-precipitable radioactivity in less than 2h.

2006 ◽  
Vol 30 (3) ◽  
pp. 319-324 ◽  
Author(s):  
I. E. Aradaib ◽  
M. E. H. Mohemmed ◽  
J. A. Sarr ◽  
S. H. Idris ◽  
N. O. M. Ali ◽  
...  

1973 ◽  
Vol 131 (3) ◽  
pp. 615-618 ◽  
Author(s):  
J. B. Smith ◽  
M. J. Silver ◽  
G. R. Webster

Substantial phospholipase A1 activity has been demonstrated in human blood platelets, and a rapid method for its measurement is described. The enzyme requires taurocholate for full activity and in these conditions the pH optimum is 4.8. The phospholipase activity is released from platelets by incubation with thrombin.


Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis

The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we are reporting the Ultrastructure of Primary Spermatocytes in tilapia, O. niloticus, and the fine structure of synaptonemal complexes seen in the spermatocyte nuclei.


Author(s):  
M. D. Vaudin ◽  
J. P. Cline

The study of preferred crystallographic orientation (texture) in ceramics is assuming greater importance as their anisotropic crystal properties are being used to advantage in an increasing number of applications. The quantification of texture by a reliable and rapid method is required. Analysis of backscattered electron Kikuchi patterns (BEKPs) can be used to provide the crystallographic orientation of as many grains as time and resources allow. The technique is relatively slow, particularly for noncubic materials, but the data are more accurate than any comparable technique when a sufficient number of grains are analyzed. Thus, BEKP is well-suited as a verification method for data obtained in faster ways, such as x-ray or neutron diffraction. We have compared texture data obtained using BEKP, x-ray diffraction and neutron diffraction. Alumina specimens displaying differing levels of axisymmetric (0001) texture normal to the specimen surface were investigated.BEKP patterns were obtained from about a hundred grains selected at random in each specimen.


1997 ◽  
Vol 61 (3) ◽  
pp. 275-279 ◽  
Author(s):  
S. MONNO ◽  
Y. MIZUSHIMA ◽  
N. TOYODA ◽  
T. KASHII ◽  
M. KOBAYASHI
Keyword(s):  

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