scholarly journals The distribution of blood-group antigens on butanol extraction of human erythrocyte ‘ghosts’

1974 ◽  
Vol 138 (3) ◽  
pp. 381-386 ◽  
Author(s):  
D. J. Anstee ◽  
M. J. A. Tanner
Keyword(s):  
Ionic Strength ◽  
Blood Group ◽  
Aqueous Phase ◽  
High Ionic Strength ◽  
High Yield ◽  
Erythrocyte Ghosts ◽  
Blood Group A ◽  
Butanol Extraction ◽  
Group A ◽  
Blood Group P

The distribution of protein and blood-group-antigen activity obtained after butanol extraction of erythrocyte `ghosts' under various conditions is described. Butanol extraction under low-ionic strength conditions results in the recovery of membrane protein in high yield in the aqueous phase. Blood-group-A activity is found in both the aqueous and butanol phases, whereas blood-group-P activity is confined to the butanol phase and blood-group-I and blood-group-MN activity are restricted to the aqueous phase. Much lower yields of protein are obtained in the aqueous phase when high-ionic-strength conditions are used. An appreciable amount of material is precipitated at the interface. Under these conditions blood-group-P activity is found only in the butanol phase, blood group-A activity in the butanol phase and interface material and only blood-group-MN activity in the aqueous phase. In contrast with previous reports no correlation could be demonstrated between the secretor status of the donors and the presence of blood-group-A activity in the aqueous phase after butanol extraction under any of the extraction conditions used. By using butanol extraction under high-ionic-strength conditions it is possible to isolate the blood-group-MN-active sialoglycoprotein in high yield from erythrocyte `ghosts' by a simple procedure.

The Half-life of Infused Factor VIII Is Shorter in Hemophiliac Patients with Blood Group 0 than in those with Blood Group A

2000 ◽  
Vol 83 (01) ◽  
pp. 65-69 ◽  
Author(s):  
Evelien Mauser-Bunschoten ◽  
Antoanette Zarkova ◽  
Els Haan ◽  
Cas Kruitwagen ◽  
Jan Sixma ◽  
...  
Keyword(s):  
Factor Viii ◽  
Blood Group ◽  
Half Life ◽  
Hemophilia A ◽  
Linear Regression Analysis ◽  
Life Time ◽  
Blood Group A ◽  
Wide Range ◽  
Group A ◽  
Blood Group P

SummaryA considerable inter-individual variation in half-life of infused factor VIII is observed among patients with hemophilia A. The factors contributing to this wide range in factor VIII half-life are not known in detail. We analysed the pharmacokinetics of infused factor VIII in 32 patients with hemophilia A, comprising 20 brothers from 10 families, 3 and 4 brothers from 2 families, and 5 patients from 5 single families, respectively. Multiple linear regression analysis was used to asses the effect of several variables on factor VIII half-life. We found that the pre-infusion von Willebrand factor antigen levels (vWF:Ag) were positively correlated with factor VIII half-life (r = 0.52, p = 0.002), i. e., each variable was associated with about 27% of the variance of the other. In fraternal pairs, familial clustering was significant for AB0 blood group (p < 0.001), but could not be detected for factor VIII half-lives or pre-infusion vWF:Ag levels. vWF:Ag level (p = 0.001) and AB0 blood group (p = 0.003) significantly determined factor VIII half-life, whereas age, length, bodyweight, the presence or absence of a factor VIII gene inversion, and Rhesus phenotype did not. Patients with blood group 0 exhibited a statistically significant shorter factor VIII half-life than patients with blood group A (15.3 versus 19.7 h, respectively) (p = 0.003). Patients with blood group A and 0 differ in respect to the presence of anti-A antibodies in the latter. It is possible that these anti-A antibodies interact with endogenous vWF, thus affecting the half-life time of the factor VIII/vWF complex.

scholarly journals Prognostic value of blood group specificity and IL-28в genotype for the assessment of liver fibrosis in patients with chronic genotype 1 hepatitis C, non-responders to the treatment with pegylated interferon α-2 and ribavarin

2017 ◽  
Vol 95 (9) ◽  
pp. 847-854
Author(s):  
Elena I. Kukhareva ◽  
P. P. Ogurtsov
Keyword(s):  
Liver Fibrosis ◽  
Blood Group ◽  
Odds Ratio ◽  
Prognostic Value ◽  
Pegylated Interferon ◽  
Main Group ◽  
Genotype 1 ◽  
Blood Group A ◽  
Group A ◽  
Blood Group P

Aim. To evaluate the prognostic value of blood group and IL-28B genotype with respect to dynamics of liver fibrosis (LF) in patients with chronic genotype-1 hepatitis C (HCV-1) who did not respond to antiviral therapy (AVT) with pegylated interferon alpha 2 (peg-IFN-α-2) and ribavarin (RBV) Material and methods. The study included 122 primary patients with HCV-1 who underwent paired liver biopsy or elastometry steam. The main group (n=66) consisted of patients who received AVT (peg-IFN-α-2/RBV) but failed to achieve a sustainable virologic response (SVR). Control group (n=56) comprised patients treated without AVT. Results. Negative dynamics of LF in patients of the main group occurred significantly more frequently than in the placebo group (p=0.025, φ2 criterion). LF dynamic patterns in patients of the main group varied depending on the IL-28B genotype and blood group, p=0.001 and p=0.014 respectively. Factorial analysis of negative LP dynamics in the main group revealed the relationship between blood group (fr=0,931) and IL-28B genotype (fr= 0.960) while classification analysis demonstrated the predictive value of combination of gene IL-28B polymorphism and blood group (p<0.0001). The assessment of probability of negative LF dynamics in the main group (logistic analysis) showed that IL-28B gene genotypes ST/TG, TT/TT,TG,GG and blood group A(II) alone or their combination increase the odds ratio of LF negative dynamics in SVR(-) under conditions of interferon therapy. In patients having blood group 0(I) and combination of gene IL-28B genotypes CC/TT, CT/TT with blood group A(II) the odds ratio of negative dynamics in SVR(-) is reduced under the same conditions. Conclusions. Blood group and IL-28B genotype predict dynamics of liver fibrosis in patients with HCV-1 not responding to interferon therapy.

scholarly journals Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

1987 ◽  
Vol 262 (29) ◽  
pp. 14228-14234
Author(s):  
H Clausen ◽  
S B Levery ◽  
E D Nudelman ◽  
M Stroud ◽  
M E Salyan ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Isolation And Characterization ◽  
Group A

scholarly journals ABO groups can play a role in susceptibility and severity of COVID-19

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
S. Samra ◽  
M. Habeb ◽  
R. Nafae
Keyword(s):  
Blood Group ◽  
Abo Blood Group ◽  
Infection Group ◽  
Mechanically Ventilated ◽  
Blood Group A ◽  
Group A ◽  
Study Population ◽  
Group B ◽  
The Relationship ◽  
Seriously Ill

Abstract Background A few people infected by the coronavirus become seriously ill, while others show little to no signs of the symptoms, or are asymptomatic. Recent researches are pointing to the fact that the ABO blood group might play an important role in a person’s susceptibility and severity of COVID-19 infection. Aim of the study: try to understand the relationship between ABO groups and COVID-19 (susceptibility and severity). Results A total of (507) patients were included in this study. The study population was divided based on the ABO blood group into types A+, A−, B+, AB, O+, and O−. Blood group A was associated with high susceptibility of infection: group A, 381 (75.1%); and less common in group O, 97 (19.2%), group B, 18 (3.5%), and group AB, 11 (2.2%). The severity of COVID-19 infection was common in non-blood group O where (20 (7.1%), 4 (26.7%), 2 (11%), and 1 (9%) in type A+, A−, B+, and AB, respectively), while in type O 3.1%. And mechanically ventilated patients were 22 (5.9%), 2 (13.4%), 2 (11.1%), and 1 (1%). Mortality was high in blood groups A and B, 16 (4.37%) and 1 (5.5%), respectively, while in blood group O, it was 1%. Conclusion The incidence, severity, and mortality of COVID-19 were common in non-blood group O. While blood group O was protected against COVID-19.

scholarly journals Blood Group O Convalescent Plasma Donations Have Significantly Lower Levels of SARS‐CoV ‐2 IgG Antibodies Compared to Blood Group A Donations

Transfusion ◽  
2021 ◽  
Author(s):  
Chelsea Hayes ◽  
Wesley Rubenstein ◽  
David Gibb ◽  
Ellen Klapper ◽  
Julie Tanaka ◽  
...  
Keyword(s):  
Blood Group ◽  
Igg Antibodies ◽  
Blood Group A ◽  
Group A

Detection of Intragraft Anti-Blood Group A and B Antibodies Following Renal Transplantation

2019 ◽  
Vol 51 (5) ◽  
pp. 1371-1377 ◽  
Author(s):  
Tsukasa Nakamura ◽  
Takayuki Shirouzu ◽  
Shintaro Kawai ◽  
Yui Imanishi ◽  
Takehisa Matsuyama ◽  
...  
Keyword(s):  
Renal Transplantation ◽  
Blood Group ◽  
Blood Group A ◽  
Group A

Blood Group A Antigen in Human Erythrocytic Membranes and Membrane Fractions

Vox Sanguinis ◽  
1977 ◽  
Vol 33 (4) ◽  
pp. 193-201 ◽  
Author(s):  
S.P. Kent ◽  
J.M. McKibbin ◽  
R. Boggio ◽  
J.B. McKibbin
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Group A
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