scholarly journals Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling

2005 ◽  
Vol 386 (2) ◽  
pp. 215-219 ◽  
Author(s):  
Patrick J. HALVEY ◽  
Walter H. WATSON ◽  
Jason M. HANSEN ◽  
Young-Mi GO ◽  
Afshin SAMALI ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Redox States ◽  
Redox Couples ◽  
Growth Factor Signalling ◽  
Thioredoxin 1 ◽  
Epidermal Growth ◽  
Cellular Oxidation ◽  
Cellular Components ◽  
Thioredoxin 2

Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.

Role of heterotrimeric G-proteins in epidermal growth factor signalling

1995 ◽  
Vol 7 (4) ◽  
pp. 303-311 ◽  
Author(s):  
Ignasi Ramírez ◽  
Francesc Tebar ◽  
Montserrat Grau ◽  
Maria Soley
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
G Proteins ◽  
Heterotrimeric G Proteins ◽  
Growth Factor Signalling ◽  
Epidermal Growth

scholarly journals Rab11-FIP2, an Adaptor Protein Connecting Cellular Components Involved in Internalization and Recycling of Epidermal Growth Factor Receptors

2002 ◽  
Vol 277 (51) ◽  
pp. 49158-49166 ◽  
Author(s):  
Donald N. Cullis ◽  
Betsey Philip ◽  
James D. Baleja ◽  
Larry A. Feig
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Sites ◽  
Adaptor Protein ◽  
Growth Factor Receptors ◽  
Epidermal Growth Factor Receptors ◽  
Recycling Endosomes ◽  
Receptor Mediated Endocytosis ◽  
Epidermal Growth ◽  
Cellular Components

Rab11-FIP2 is a member of a newly identified family of Rab11-binding proteins that have been implicated in the function of recycling endosomes. Here we show that Rab11-FIP2 may also be involved with the process of receptor-mediated endocytosis. First we demonstrate that Rab11-FIP2 contains an NPF motif that allows it to bind Reps1, a member of a family of EH domain proteins involved in endocytosis. We also show that Rab11-FIP2 associates with the α-adaptin subunit of AP-2 complexes, which are known to recruit receptors into clathrin-coated vesicles. Finally, we find that overexpression of Rab11-FIP2 suppresses the internalization of epidermal growth factor receptors, but not transferrin receptors, through binding sites that promote complex formation with Rab11, Reps1, and α-adaptin. These findings suggest that Rab11-FIP2 may participate in the coupling of receptor-mediated endocytosis to the subsequent sorting of receptor-containing vesicles in endosomes.

scholarly journals Epidermal Growth Factor Signalling Controls Myosin II Planar Polarity to Orchestrate Convergent Extension Movements during Drosophila Tubulogenesis

PLoS Biology ◽  
2014 ◽  
Vol 12 (12) ◽  
pp. e1002013 ◽  
Author(s):  
Aditya Saxena ◽  
Barry Denholm ◽  
Stephanie Bunt ◽  
Marcus Bischoff ◽  
Krishnaswamy VijayRaghavan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Myosin Ii ◽  
Convergent Extension ◽  
Planar Polarity ◽  
Growth Factor Signalling ◽  
Epidermal Growth
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