scholarly journals Effects of steroid hormones and xenobiotics on the pubertal development of UDP-glucuronosyltransferase activities towards androsterone and 4-nitrophenol in Wistar rats

1984 ◽  
Vol 222 (2) ◽  
pp. 321-326 ◽  
Author(s):  
H K Watanabe ◽  
M Matsui
Keyword(s):  
High Activity ◽  
Steroid Hormones ◽  
Wistar Rats ◽  
Pubertal Development ◽  
Suppressive Effect ◽  
Feeding Experiments ◽  
Oestradiol Benzoate ◽  
Old Rats ◽  
Udp Glucuronosyltransferase ◽  
Genetically Determined

Oestradiol benzoate, testosterone propionate, progesterone, corticosterone, 3-methylcholanthrene and phenobarbital were administered to Wistar rats at the pubertal period, and their effects on hepatic UDP-glucuronosyltransferase activities were determined. Pretreatment with oestradiol benzoate had a temporary suppressive effect on androsterone UDP-glucuronosyltransferase activity in rats with the high-activity phenotype of androsterone glucuronidation. The effect was marked in 40-day-old rats, but was not found in older rats. Androsterone UDP-glucuronosyltransferase activity was induced by phenobarbital in rats with the high-activity phenotype, but not in rats with the low-activity phenotype. Foster-feeding experiments showed that breast milk did not alter the genetically determined expression of androsterone UDP-glucuronosyltransferase activity in Wistar rats. In contrast, 4-nitrophenol UDP-glucuronosyltransferase activity was not affected by steroid hormones, but was highly induced by 3-methylcholanthrene.

scholarly journals Classification and genetic expression of Wistar rats with high and low hepatic microsomal UDP-glucuronosyltransferase activity towards androsterone

1982 ◽  
Vol 202 (1) ◽  
pp. 171-174 ◽  
Author(s):  
Michio Matsui ◽  
Hiroshi K. Watanabe
Keyword(s):  
High Activity ◽  
Wistar Rats ◽  
Autosomal Dominant ◽  
Transferase Activity ◽  
Male And Female ◽  
Genetic Expression ◽  
Autosomal Dominant Fashion ◽  
Female Wistar Rats ◽  
Udp Glucuronosyltransferase ◽  
Low Activity

Male and female Wistar rats with high and low hepatic microsomal UDP-glucuronosyltransferase activity towards androsterone were classified by partial hepatectomy. The breeding experiments between the classified high-activity and low-activity rats show that the genetic expression of the high transferase activity is inherited in an autosomal dominant fashion.

scholarly journals Genetic deficiency of androsterone UDP-glucuronosyltransferase activity in Wistar rats is due to the loss of enzyme protein

1986 ◽  
Vol 234 (1) ◽  
pp. 139-144 ◽  
Author(s):  
M Matsui ◽  
F Nagai
Keyword(s):  
Affinity Chromatography ◽  
High Activity ◽  
Wistar Rats ◽  
Enzyme Protein ◽  
Genetic Deficiency ◽  
A Subunit ◽  
Udp Glucuronosyltransferase ◽  
Low Activity ◽  
Hydroxy Steroid

Hepatic microsomal UDP-glucuronosyltransferases towards androsterone and testosterone were purified by chromatofocusing and UDP-hexanolamine affinity chromatography in Wistar rats which had genetic deficiency of androsterone UDP-glucuronosyltransferase activity. In rats with the high-activity phenotype, androsterone (the 3-hydroxy androgen) UDP-glucuronosyltransferase was eluted at about pH 7.4 and had a subunit Mr of 52 000, whereas testosterone (the 17-hydroxy steroid) UDP-glucuronosyltransferase was eluted at about pH 8.4 and had a subunit Mr of 50 000. The transferase that conjugates both androsterone and testosterone was eluted at about pH 8.0, had subunit Mr values of 50 000 and 52 000, and appeared to be an aggregate or hybrid of androsterone and testosterone UDP-glucuronosyltransferases. In rats with the low-activity phenotype, androsterone UDP-glucuronosyltransferase was absent, whereas testosterone UDP-glucuronosyltransferase was eluted at around pH 8.5, with a subunit Mr of 50 000.

Light and electron microscopic investigation of adenohypophyses of germfree, food-restricted, and germfree-food restricted aging, male lobund-wistar rats

1988 ◽  
Vol 46 ◽  
pp. 352-353
Author(s):  
G. Ilse ◽  
K. Kovacs ◽  
N. Ryan ◽  
T. Sano ◽  
L. Stefaneanu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Microscopic Investigation ◽  
Electron Microscopic Investigation ◽  
Aging Male ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Sprague Dawley Rats ◽  
Old Rats ◽  
Ad Libitum ◽  
Microscopic Findings

Germfree state and food restriction have been shown to increase life span and delay tumor occurrence in rats. We report here the histologic, immunocytochemical and electron microscopic findings of adenohypophyses of aging, male Lobund-Wistar rats raised at Lobund Laboratories. In our previous study, the morphologic changes in the adenohypophyses of old rats have been extensively investigated by histology, immunocytochemistry and electron microscopy. Lactotroph adenomas were frequent in Long-Evans and Sprague-Dawley rats, whereas gonadotroph adenomas were frequent in Sprague-Dawley and Wistar rats.Male Lobund-Wistar rats were divided into four groups: 1) conventional, which were raised under normal non-germfree environment and received food ad libitum; 2) germfree-food ad libitum; 3) conventional environment-food restricted and 4) germfree-food restricted. The adenohypophyses were removed from 6-month-, 18-month- and 30-month-old rats. For light microscopy, adenohypophyses were fixed in formalin and embedded in paraffin.

scholarly journals Urinary bisphenol A in women with polycystic ovary syndrome – a possible suppressive effect on steroidogenesis?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Zora Lazúrová ◽  
Jana Figurová ◽  
Beáta Hubková ◽  
Jana Mašlanková ◽  
Ivica Lazúrová
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Bisphenol A ◽  
Steroid Hormones ◽  
Polycystic Ovary ◽  
Suppressive Effect ◽  
Human Reproduction ◽  
Control Group ◽  
Ovary Syndrome ◽  
Ovarian Steroidogenesis ◽  
The Relationship

Abstract Objectives There is a growing evidence indicating an impact of endocrine distrupting chemicals such as bisphenol A (BPA) on human reproduction. Its higher levels in serum or urine have been documented in women with polycystic ovary syndrome (PCOS), however the relationship to ovarian steroidogenesis remains unclear. Aim of the study was to compare urinary BPA (U-BPA) concentrations among PCOS women and control group. Second aim was to assess the relationship of U-BPA to ovarian steroidogenesis in the group with PCOS. Methods Eighty six Caucasian women (age 28.5 ± 5.1 years) diagnosed with PCOS and 32 controls of age 24.9 ± 4.4 years were included in the study. Fasting blood samples were analyzed for biochemical parameters and steroid hormones. U-BPA was measured in the morning urine sample using high pressure liquid chromatography. Results PCOS women had significantly higher U-BPA as compared with control group (p=0.0001). Those with high levels of U-BPA (U-BPA ≥2.14 ug/g creatinine) demonstrated higher serum insulin (p=0.029) and HOMA IR (p=0.037), lower serum estrone (p=0.05), estradiol (p=0.0126), FSH (p=0.0056), and FAI (p=0.0088), as compared with low-BPA group (U- BPA <2.14 ug/g creatinine). In PCOS women, U-BPA positively correlated with age (p=0.0026; R2=0.17), negatively with estradiol (p=0.0001, R2=0.5), testosterone (p=0.0078, R2=0.15), free-testosterone (p=0.0094, R2=0.12) and FAI (p=0.0003, R2=0.32), respectively. Conclusions PCOS women have significantly higher U-BPA concentrations than healthy controls. U-BPA positively correlates with age and negatively with ovarian steroid hormones suggesting a possible suppressive effect of bisphenol A on ovarian steroidogenesis.

Neonatal exposure to herbicide acetochlor alters pubertal development in female wistar rats

2011 ◽  
Vol 21 (5) ◽  
pp. 406-417 ◽  
Author(s):  
Eva Rollerova ◽  
Ladislava Wsolova ◽  
Miroslava Urbancikova
Keyword(s):  
Wistar Rats ◽  
Pubertal Development ◽  
Neonatal Exposure ◽  
Female Wistar Rats

Age-dependent reduction in insulin secretion and insulin mRNA in isolated islets from rats

1995 ◽  
Vol 269 (6) ◽  
pp. E983-E990 ◽  
Author(s):  
R. Perfetti ◽  
C. M. Rafizadeh ◽  
A. S. Liotta ◽  
J. M. Egan
Keyword(s):  
Beta Cell ◽  
Wistar Rats ◽  
Cell Activity ◽  
Insulin Content ◽  
Dependent Diabetes Mellitus ◽  
Etiologic Factor ◽  
Mrna Levels ◽  
Age Dependent ◽  
Insulin Mrna ◽  
Old Rats

Aging is an etiologic factor in non-insulin-dependent diabetes mellitus. To characterize the beta-cell abnormalities that occur with age, we investigated glucose-stimulated insulin release, pancreatic insulin content, and mRNA levels for islet-specific genes in aging Wistar rats. Ten minutes after glucose stimulation, 6-mo-old islets had approximately 40% more cells secreting insulin than 24-mo-old islets (P < 0.0001); after 1 h, 67 +/- 1.0% islets from 6-mo-old rats secreted insulin, compared with 51 +/- 3.5% from 24-mo-old rats (P < 0.0001). The amount of insulin secreted by each beta-cell was also less in the older animals (P < 0.0001). Despite increases in islet size (P < 0.0001) and beta-cell number (P < 0.0001) with age, whole pancreas insulin content showed that 24-mo-old pancreas had less insulin than 6-mo-old pancreas (0.61 +/- 0.06 vs. 0.84 +/- 0.08 microgram/mg pancreatic protein; P < 0.05). Finally, insulin mRNA levels declined to 50% of the newborn value in 24-mo-old islets (P < 0.0001), whereas glucagon mRNA levels showed a very modest decline with age. Somatostatin mRNA levels did not vary significantly. In summary, it appears that in Wistar rats there is a progressive decline in beta-cell activity with age. This decline may represent the biological features of the age-dependent risk of developing diabetes.

Effect Of Bilateral Ovariectomy And Ovarian Steroid Hormones On The Antioxidant Systems And Plasma Malondialdehyde Levels In Wistar Rats

2000 ◽  
Vol 26 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Ma Gómez-zubeldia ◽  
R Hernandez ◽  
J Viguera ◽  
Jj Arbues ◽  
A Aparicio ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Wistar Rats ◽  
Antioxidant Systems ◽  
Ovarian Steroid ◽  
Bilateral Ovariectomy ◽  
Plasma Malondialdehyde

Effects of acetazolamide and metabolic acidosis and alkalosis upon gastric acid secretion

1962 ◽  
Vol 202 (3) ◽  
pp. 429-436 ◽  
Author(s):  
Frank M. Byers ◽  
Paul H. Jordan ◽  
Thomas H. Maren
Keyword(s):  
Metabolic Acidosis ◽  
Carbonic Anhydrase ◽  
Acid Secretion ◽  
Metabolic Alkalosis ◽  
Suppressive Effect ◽  
Low Doses ◽  
Heidenhain Pouch ◽  
Feeding Experiments ◽  
Initial Reduction ◽  
Higher Doses

The gastric H+ secretory responses to i.v. HCl, NaHCO3, and acetazolamide were studied in Heidenhain-pouch dogs that had been stimulated to secrete by feeding. Acetazolamide in low doses (5 mg/kg) gave a 65% reduction in H+ output for 2–4 hr, after which H+ secretion increased above normal, coincident with renal HCO3– loss and metabolic acidosis. Higher doses produced a greater systemic acidosis and resulted in less initial reduction of H+ and greater augmentation. Doses of 75–100 mg/kg, given 17 hr before feeding to produce a systemic acidosis, resulted in increased secretion, even though sufficient drug remained in the plasma to inhibit tissue carbonic anhydrase. Metabolic acidosis (i.v. HCl) initially suppressed but later augmented gastric H+. Acetazolamide had no suppressive effect in the presence of either an HCl- or acetazolamide-induced acidosis. Metabolic alkalosis (i.v. NaHCO3) reduced gastric H+ in regular feeding experiments and in dogs made acidotic by acetazolamide.

scholarly journals Cell-Free DNA Plasma Levels Differ in Age-Specific Pattern in Healthy Rats and Castrates with Testosterone-Induced Benign Prostatic Hyperplasia

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
I. N. Vasilyeva ◽  
V. G. Bespalov ◽  
J. D. Von ◽  
A. L. Semenov ◽  
G. V. Tochilnikov ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Wistar Rats ◽  
Specific Pattern ◽  
Prostatic Hyperplasia ◽  
The Body ◽  
Cell Free Dna ◽  
Young Rats ◽  
Free Dna ◽  
Male Wistar Rats ◽  
Old Rats

The purpose of this work was to study changes in the level of cell-free DNA (cfDNA) in the blood of young and old rats in the normal state and with induced benign prostatic hyperplasia (BPH). Male Wistar rats were divided into 4 groups—young (3 months), old (20 months), intact, or with testosterone-induced BPH. Groups with BPH were subjected to surgical castration and administration of testosterone esters at a dose of 25 mg/kg for a total of 7 injections for 20 days. In intact animals, the level of cfDNA in old rats (2.00±0.14 ng/μl) was significantly higher than that in the young (1.02±0.30 ng/μl). The body and the prostate weights of old rats were 1.6 and 1.4 times larger than those of the young, without an increase in the prostate index (PI). The testosterone level in the blood of young rats was 1.6 times higher than that of old (6.20±0.93 nmol/l vs. 3.77±0.55 nmol/l; NS). In animals with BPH, the level of cfDNA in old rats (3.14±0.76 ng/μl) was significantly higher than that in young rats (0.80±0.14 ng/μl). The body and the prostate weights in old rats were 1.8 and 2.3 times larger, than those in young rats, with an increase in the PI. The level of testosterone in the blood of young (15.76±0.51 nmol/l) and old (16.99±1.1 nmol/l) rats was not significantly different. Morphological signs of BPH were observed in the prostate of both young and old rats. During the induction of BPH in the experiment, according to the level of cfDNA, cell death processes have not changed significantly in young rats but significantly increased in old rats. A similar trend was observed in the group of intact animals. The obtained data indicate that apoptosis processes are enhanced during the development of BPH despite the growth of tissues in the prostate itself.

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