Induced oxidative stress and activated expression of manganese superoxide dismutase during hepatitis C virus replication: role of JNK, p38 MAPK and AP-1

2004 ◽  
Vol 381 (3) ◽  
pp. 951-951
Author(s):  
I. QADRI ◽  
M. IWAHASHI ◽  
J. M. CAPASSO ◽  
M. W. HOPKEN ◽  
S. FLORES ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
P38 Mapk ◽  
Virus Replication ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide

scholarly journals Induced oxidative stress and activated expression of manganese superoxide dismutase during hepatitis C virus replication: role of JNK, p38 MAPK and AP-1

2004 ◽  
Vol 378 (3) ◽  
pp. 919-928 ◽  
Author(s):  
Ishtiaq QADRI ◽  
Mieko IWAHASHI ◽  
Juan M. CAPASSO ◽  
Matthew W. HOPKEN ◽  
Sonia FLORES ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
P38 Mapk ◽  
Cellular Response ◽  
Manganese Superoxide Dismutase ◽  
Fold Increase ◽  
Subgenomic Replicon ◽  
Manganese Superoxide

Activation of cellular kinases and transcription factors mediates the early phase of the cellular response to chemically or biologically induced stress. In the present study we investigated the oxidant/antioxidant balance in Huh-7 cells expressing the HCV (hepatitis C virus) subgenomic replicon, and observed a 5-fold increase in oxidative stress during HCV replication. We used MnSOD (manganese-superoxide dismutase) as an indicator of the cellular antioxidant response, and found that its activity, protein levels and promoter activity were significantly increased, whereas Cu/ZnSOD was not affected. The oxidative stress-induced protein kinases p38 MAPK (mitogen-activated protein kinase) and JNK (c-Jun N-terminal kinase) were activated in the HCV repliconcontaining cells and in Huh-7 cells transduced with Ad-NS5A [a recombinant adenovirus encoding NS5A (non-structural protein 5A)], coupled with a 4–5-fold increase in AP-1 (activator protein-1) DNA binding. Ava.1 cells, which encode a replication-defective HCV replicon, showed no significant changes in MnSOD, p38 MAPK or JNK activity. The AP-1 inhibitors dithiothreitol and N-acetylcysteine, as well as a dominant negative AP-1 mutant, significantly reduced AP-1 activation, demonstrating that this activation is oxidative stress-related. Exogenous NS5A had no effect on AP-1 activation in vitro, suggesting that NS5A acts at the upstream targets of AP-1 involving p38 MAPK and JNK signalling cascades. AP-1-dependent gene expression was increased in HCV subgenomic replicon-expressing Huh-7 cells. MnSOD activation was blocked by inhibitors of JNK (JNKI1) and p38 MAPK (SB203580), but not by an ERK (extracellular-signal-regulated kinase) inhibitor (U0126), in HCV-replicating and Ad-NS5A-transduced cells. Our results demonstrate that cellular responses to oxidative stress in HCV subgenomic replicon-expressing and Ad-NS5A-transduced cells are regulated by two distinct signalling pathways involving p38 MAPK and JNK via AP-1 that is linked to increased oxidative stress and therefore to an increased antioxidant MnSOD response.

Manganese Superoxide Dismutase Dimorphism and Iron Overload, Hepatocellular Carcinoma, and Death in Hepatitis C Virus–Infected Patients

2007 ◽  
Vol 5 (5) ◽  
pp. 630-635 ◽  
Author(s):  
Pierre Nahon ◽  
Angela Sutton ◽  
Dominique Pessayre ◽  
Pierre Rufat ◽  
Marianne Ziol ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Superoxide Dismutase ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Iron Overload ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide

Role of manganese superoxide dismutase in a mucoid isolate of Pseudomonas aeruginosa: adaptation to oxidative stress.

1996 ◽  
Vol 64 (6) ◽  
pp. 2216-2219 ◽  
Author(s):  
B Polack ◽  
D Dacheux ◽  
I Delic-Attree ◽  
B Toussaint ◽  
P M Vignais
Keyword(s):  
Oxidative Stress ◽  
Pseudomonas Aeruginosa ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide

scholarly journals Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene

2015 ◽  
Vol 309 (3) ◽  
pp. C169-C178 ◽  
Author(s):  
Guiming Liu ◽  
Rania A. Elrashidy ◽  
Nan Xiao ◽  
Michael Kavran ◽  
Yexiang Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Smooth Muscle ◽  
Manganese Superoxide Dismutase ◽  
Bladder Function ◽  
Antioxidant Systems ◽  
Wild Type ◽  
Manganese Superoxide ◽  
Specific Deletion

Manganese superoxide dismutase (MnSOD) is considered a critical component of the antioxidant systems that protect against oxidative damage. We are interested in the role of oxidative stress in bladder detrusor smooth muscle (SM) in different disease states. In this study, we generated an inducible, SM-specific Sod2−/− mouse model to investigate the effects of MnSOD depletion on the function of the bladder. We crossbred floxed Sod2 ( Sod2lox/lox) mice with mice containing heterozygous knock-in of a gene encoding a tamoxifen-activated Cre recombinase in the SM22α promoter locus [SM-CreERT2(ki)Cre/+]. We obtained Sod2lox/lox,SM-CreERT2(ki)Cre/+ mice and injected 8-wk-old males with 4-hydroxytamoxifen to induce Cre-mediated excision of the floxed Sod2 allele. Twelve weeks later, SM-specific deletion of Sod2 and depletion of MnSOD were confirmed by polymerase chain reaction, immunoblotting, and immunohistochemistry. SM-specific Sod2−/− mice exhibited normal growth with no gross abnormalities. A significant increase in nitrotyrosine concentration was found in bladder SM tissue of SM-specific Sod2−/− mice compared with both wild-type mice and Sod2+/+, SM-CreERT2(ki)Cre/+ mice treated with 4-hydroxytamoxifen. Assessment of 24-h micturition in SM-specific Sod2−/− mice revealed significantly higher voiding frequency compared with both wild-type and SM-specific Cre controls. Conscious cystometry revealed significantly shorter intercontraction intervals and lower functional bladder capacity in SM-specific Sod2−/− mice compared with wild-type mice. This novel model can be used for exploring the mechanistic role of oxidative stress in organs rich in SM in different pathological conditions.

Heterozygous Deficiency of Manganese Superoxide Dismutase in Mice (Mn-SOD+/-): A Novel Approach to Assess the Role of Oxidative Stress for the Development of Nitrate Tolerance

2005 ◽  
Vol 68 (3) ◽  
pp. 579-588 ◽  
Author(s):  
Andreas Daiber ◽  
Matthias Oelze ◽  
Silke Sulyok ◽  
Meike Coldewey ◽  
Eberhard Schulz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Nitrate Tolerance ◽  
Novel Approach ◽  
Manganese Superoxide ◽  
Heterozygous Deficiency

Role of autophagy in hepatitis C virus replication

2019 ◽  
Author(s):  
WI Twu ◽  
K Tabata ◽  
D Paul ◽  
R Bartenschlager
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication

scholarly journals A Case-Control Study Exploring the Role of Serum Manganese Superoxide Dismutase (MnSOD) Levels in Gastric Cancer

2005 ◽  
Vol 15 (3) ◽  
pp. 90-95 ◽  
Author(s):  
Yingsong Lin ◽  
Shogo Kikuchi ◽  
Yuki Obata ◽  
Kiyoko Yagyu
Keyword(s):  
Gastric Cancer ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Case Control Study ◽  
Case Control ◽  
Manganese Superoxide ◽  
Control Study
Sign in / Sign up

Export Citation Format

Share Document