Effect of growth factors on fibroblast migration in a simplified wound healing model

Wound healing is a very complex process that allows organisms to survive injuries. It is strictly regulated by a number of biochemical and physical factors, mechanical forces included. Studying wound healing in space is interesting for two main reasons: (i) defining tools, procedures, and protocols to manage serious wounds and burns eventually occurring in future long-lasting space exploration missions, without the possibility of timely medical evacuation to Earth; (ii) understanding the role of gravity and mechanical factors in the healing process and scarring, thus contributing to unravelling the mechanisms underlying the switching between perfect regeneration and imperfect repair with scarring. In the study presented here, a new in vivo sutured wound healing model in the leech (Hirudo medicinalis) has been used to evaluate the effect of unloading conditions on the healing process and the effectiveness of platelet rich plasma (PRP) as a countermeasure. The results reveal that microgravity caused a healing delay and structural alterations in the repair tissue, which were prevented by PRP treatment. Moreover, investigating the effects of microgravity and PRP on an in vitro wound healing model, it was found that PRP is able to counteract the microgravity-induced impairment in fibroblast migration to the wound site. This could be one of the mechanisms underlying the effectiveness of PRP in preventing healing impairment in unloading conditions.

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Butter oil (ghee) enrichment with aromatic plants: Chemical characterization and effects on fibroblast migration in anin-vitro wound healing model

Arabian Journal of Chemistry ◽

10.1016/j.arabjc.2020.10.017 ◽

2020 ◽

Vol 13 (12) ◽

pp. 8909-8919

Author(s):

Afraa Maiza ◽

Franks Kamgang Nzekoue ◽

Tesmine Ghazouani ◽

Makrem Afif ◽

Giovanni Caprioli ◽

...

Keyword(s):

Wound Healing ◽

Chemical Characterization ◽

Aromatic Plants ◽

Butter Oil ◽

Fibroblast Migration ◽

Wound Healing Model ◽

Healing Model

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Interaction of TGF-β1 and Ionizing Radiation in an In Vitro Wound Healing Model: Rho Kinase-mediated Inhibition of Fibroblast Migration and Implications for IORT

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2011.06.1355 ◽

2011 ◽

Vol 81 (2) ◽

pp. S708

Author(s):

L. Ma ◽

C. Kettner ◽

F. Wenz ◽

C. Herskind

Keyword(s):

Wound Healing ◽

Ionizing Radiation ◽

Rho Kinase ◽

Fibroblast Migration ◽

Wound Healing Model ◽

Healing Model ◽

Tgf Β1

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Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

Experimental Cell Research ◽

10.1016/j.yexcr.2013.09.016 ◽

2014 ◽

Vol 320 (1) ◽

pp. 79-91 ◽

Cited By ~ 14

Author(s):

Nina Tandon ◽

Elisa Cimetta ◽

Aranzazu Villasante ◽

Nicolette Kupferstein ◽

Michael D. Southall ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Wound Healing ◽

Reactive Oxygen ◽

Dermal Fibroblasts ◽

Oxygen Species ◽

Human Dermal Fibroblasts ◽

Wound Healing Model ◽

Healing Model

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Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response

Genes ◽

10.3390/genes12010047 ◽

2020 ◽

Vol 12 (1) ◽

pp. 47

Author(s):

Carlos León ◽

Francisco García-García ◽

Sara Llames ◽

Eva García-Pérez ◽

Marta Carretero ◽

...

Keyword(s):

Wound Healing ◽

Functional Enrichment ◽

Preclinical Model ◽

Published Data ◽

Diabetic Patients ◽

Ulcer Formation ◽

Histological Features ◽

Depth Analysis ◽

Wound Healing Model ◽

Healing Model

Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients’ quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.

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