Alkaline cyanide biodegradation by Pseudomonas pseudoalcaligenes CECT5344

2005 ◽  
Vol 33 (1) ◽  
pp. 168-169 ◽  
Author(s):  
V.M. Luque-Almagro ◽  
R. Blasco ◽  
M.J. Huertas ◽  
M. Martínez-Luque ◽  
C. Moreno-Vivián ◽  
...  
Keyword(s):  
Waste Water ◽  
Heavy Metal ◽  
Alternative Oxidase ◽  
Iron Acquisition ◽  
Nitrogen Sources ◽  
Degradation Pathway ◽  
Pseudomonas Pseudoalcaligenes ◽  
Metal Cyanide ◽  
Alkaline Conditions ◽  
Cyanide Degradation

Pseudomonas pseudoalcaligenes CECT5344 uses cyanide, cyanate, β-cyanoalanine, and other cyanoderivatives as nitrogen sources under alkaline conditions, which prevents volatile HCN (pKa 9.2) formation. The cyanide consumed by this strain is stoichiometrically converted into ammonium. In addition, this bacterium grows with the heavy metal, cyanide-containing waste water generated by the jewellery industry, and is also a cyanide-resistant strain which induces an alternative oxidase and a siderophore-based mechanism for iron acquisition in the presence of cyanide. The detection of cyanase and β-cyanoalanine nitrilase activities in cyanide-induced cells suggests their implication in the cyanide degradation pathway.

scholarly journals Bacterial Degradation of Cyanide and Its Metal Complexes under Alkaline Conditions

2005 ◽  
Vol 71 (2) ◽  
pp. 940-947 ◽  
Author(s):  
Víctor M. Luque-Almagro ◽  
María-J. Huertas ◽  
Manuel Martínez-Luque ◽  
Conrado Moreno-Vivián ◽  
M. Dolores Roldán ◽  
...  
Keyword(s):  
Metal Complexes ◽  
Nitrogen Source ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Degradation Pathway ◽  
Alkaline Media ◽  
Bacterial Degradation ◽  
16S Rna ◽  
Alkaline Conditions ◽  
Cyanide Degradation

ABSTRACT A bacterial strain able to use cyanide as the sole nitrogen source under alkaline conditions has been isolated. The bacterium was classified as Pseudomonas pseudoalcaligenes by comparison of its 16S RNA gene sequence to those of existing strains and deposited in the Colección Española de Cultivos Tipo (Spanish Type Culture Collection) as strain CECT5344. Cyanide consumption is an assimilative process, since (i) bacterial growth was concomitant and proportional to cyanide degradation and (ii) the bacterium stoichiometrically converted cyanide into ammonium in the presence of l-methionine-d,l-sulfoximine, a glutamine synthetase inhibitor. The bacterium was able to grow in alkaline media, up to an initial pH of 11.5, and tolerated free cyanide in concentrations of up to 30 mM, which makes it a good candidate for the biological treatment of cyanide-contaminated residues. Both acetate and d,l-malate were suitable carbon sources for cyanotrophic growth, but no growth was detected in media with cyanide as the sole carbon source. In addition to cyanide, P. pseudoalcaligenes CECT5344 used other nitrogen sources, namely ammonium, nitrate, cyanate, cyanoacetamide, nitroferricyanide (nitroprusside), and a variety of cyanide-metal complexes. Cyanide and ammonium were assimilated simultaneously, whereas cyanide strongly inhibited nitrate and nitrite assimilation. Cyanase activity was induced during growth with cyanide or cyanate, but not with ammonium or nitrate as the nitrogen source. This result suggests that cyanate could be an intermediate in the cyanide degradation pathway, but alternative routes cannot be excluded.

Cyanide metabolism of Pseudomonas pseudoalcaligenes CECT5344: role of siderophores

2006 ◽  
Vol 34 (1) ◽  
pp. 152-155 ◽  
Author(s):  
M.-J. Huertas ◽  
V.M. Luque-Almagro ◽  
M. Martínez-Luque ◽  
R. Blasco ◽  
C. Moreno-Vivián ◽  
...  
Keyword(s):  
Metal Complexes ◽  
Protein Function ◽  
Iron Acquisition ◽  
Uptake System ◽  
Free Cyanide ◽  
Isolation And Identification ◽  
Pseudomonas Pseudoalcaligenes ◽  
Proteomic Approach ◽  
Cyanide Degradation ◽  
Cyanide Metabolism

Cyanide is one of the most potent and toxic chemicals produced by industry. The jewellery industry of Córdoba (Spain) generates a wastewater (residue) that contains free cyanide, as well as large amounts of cyano–metal complexes. Cyanide is highly toxic to living systems because it forms very stable complexes with transition metals that are essential for protein function. In spite of its extreme toxicity, some organisms have acquired mechanisms to avoid cyanide poisoning. The biological assimilation of cyanide needs the concurrence of three separate processes: (i) a cyanide-insensitive respiratory chain, (ii) a system for iron acquisition (siderophores) and (iii) a cyanide assimilation pathway. Siderophores are low-molecular-mass compounds (600–1500 Da) that scavenge iron (Fe3+) ions (usually with extremely high affinity) from the environment under iron-limiting conditions. There are two main classes of siderophores: catechol and hydroxamate types. The catechol-type siderophores chelate ferric ion via a hydroxy group, whereas the hydroxamate-type siderophores bind iron via a carbonyl group with the adjacent nitrogen. In the presence of cyanide, bacterial proliferation requires this specific metal uptake system because siderophores are able to break down cyano–metal complexes. Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide or cyano–metal complexes as nitrogen source. A proteomic approach was used for the isolation and identification, in this strain, of a protein that was induced in the presence of cyanide, namely CN0, that is involved in siderophore biosynthesis in response to cyanide. An overview of bacterial cyanide degradation pathways and the involvement of siderophores in this process are presented.

Abatement of Heavy Metal from Waste Water Using Surface Modified Adsorbent

2017 ◽  
Author(s):  
Hunge Sudhir ◽  
Rahangdale Pralhad ◽  
Lanjewar Mamata
Keyword(s):  
Waste Water ◽  
Heavy Metal ◽  
Surface Modified

Utilization of Polysaccharides and their Derivatives in the Removal of Metal Ions: Role and Recent Advancement

2020 ◽  
Vol 13 ◽  
Author(s):  
Rishabha Malviya ◽  
Pramod Sharma ◽  
Akanksha Sharma
Keyword(s):  
Waste Water ◽  
Heavy Metal ◽  
Metal Ions ◽  
Industrial Waste ◽  
Heavy Metal Ions ◽  
Environmental Hazards ◽  
Industrial Waste Water ◽  
Recent Advancement ◽  
Removal Of Metal ◽  
Removal Of Metal Ions

: Manuscript discussed about the role of polysaccharides and their derivatives in the removal of metal ions from industrial waste water. Quick modernization and industrialization increases the amount of various heavy metal ions in the environment. They can possess various disease in humans and also causes drastic environmental hazards. In this review the recent advancement for the adsorption of heavy metal ions from waste water by using different methods has been studied. Various natural polymers and their derivatives are act as effective adsorbents for the removal of heavy metal ions from the waste water released from the industries and the treated water released into the environment can decreases the chances of diseases in humans and environmental hazards. From the literature surveys it was concluded that the removal of heavy metal ions from the industrial waste water was important to decrease the environmental pollution and also diseases caused by the heavy metal ions. Graft copolymers were acts as most efficient adsorbent for the removal of heavy metal ions and most of these followed the pseudo first order and pseudo second order model of kinetics.

scholarly journals Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP

2003 ◽  
Vol 69 (12) ◽  
pp. 6987-6993 ◽  
Author(s):  
Vicente García-González ◽  
Fernando Govantes ◽  
Liz J. Shaw ◽  
Richard G. Burns ◽  
Eduardo Santero
Keyword(s):  
Wild Type Strain ◽  
Nitrogen Sources ◽  
Degradation Pathway ◽  
Strong Impact ◽  
Soil Microcosms ◽  
Degradation Rates ◽  
Herbicide Atrazine ◽  
Mutant Derivative ◽  
Nitrogen Repression ◽  
Atrazine Degradation

ABSTRACT Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated on growth-limiting nitrogen sources. The presence of atrazine in addition to the nitrogen sources did not stimulate degradation. High degradation rates obtained in the presence of ammonium plus the glutamine synthetase inhibitor MSX and also with an Nas− mutant derivative grown on nitrate suggest that nitrogen regulation operates by sensing intracellular levels of some key nitrogen-containing metabolite. Nitrate amendment in soil microcosms resulted in decreased atrazine mineralization by the wild-type strain but not by the Nas− mutant. This suggests that, although nitrogen repression of the atrazine catabolic pathway may have a strong impact on atrazine biodegradation in nitrogen-fertilized soils, the use of selected mutant variants may contribute to overcoming this limitation.

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