Effects of dietary fish oil supplementation on the fatty acid composition of the human platelet membrane: demonstration of selectivity in the incorporation of eicosapentaenoic acid into membrane phospholipid pools

1985 ◽  
Vol 68 (4) ◽  
pp. 449-454 ◽  
Author(s):  
John H. Galloway ◽  
Ian J. Cartwright ◽  
Barrie E. Woodcock ◽  
Michael Greaves ◽  
R. Graham ◽  
...  

1. The fatty acid composition of membrane phospholipids of stimulated and unstimulated platelets was studied in six normal volunteers given a daily dietary supplement of a fish oil rich in eicosapentaenoic acid (EPA) for 4 weeks. The supplement was equivalent to 1.8 g of EPA daily. Thromboxane synthesis and platelet aggregation responses to sodium arachidonate, thrombin and the ionophore A23187 were also investigated. 2. A marked increase in the relative EPA content of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was noted after 2 and 4 weeks fish oil supplementation. However, there was no incorporation into phosphatidylinositol (PI) or phosphatidylserine (PS). The relative arachidonic acid (AA) content of PC and PE was significantly reduced at 2 and 4 weeks but that of PI and PS remained unchanged. Significant reductions in the relative linoleic acid content of total phospholipids, PC and PE were also noted. 3. Stimulation of platelets obtained after 4 weeks fish oil supplementation by thrombin and A23187 was associated with a marked reduction in the AA content of PI and a minor reduction in that of PE. There was no change in the relative proportions of EPA in PI, PS, PC or PE after stimulation. Throughout the study there were no significant changes in platelet aggregation responses or in platelet thromboxane production. 4. Our results indicate that the incorporation of EPA into the platelet membrane phospholipids is selective and that if PI is the major source of AA for platelet prostaglandin biosynthesis then the reported beneficial effects of EPA on haemostasis cannot be explained on the basis of its incorporation into and mobilization from the platelet membrane phospholipid pool.

Lipids ◽  
1987 ◽  
Vol 22 (10) ◽  
pp. 744-750 ◽  
Author(s):  
Trevor A. Mori ◽  
James P. Codde ◽  
Robert Vandongen ◽  
Lawrence J. Beilin

2007 ◽  
Vol 98 (6) ◽  
pp. 1143-1151 ◽  
Author(s):  
Sébastien Judé ◽  
Eric Martel ◽  
Fanny Vincent ◽  
Pierre Besson ◽  
Charles Couet ◽  
...  

The effects of an n-3 PUFA-enriched diet on cardiac cell membrane phospholipid fraction compositions and associated protein kinase-C (PKC) translocation modification have never been studied in higher mammals. This is of importance since membrane fatty acid composition has been shown to influence PKC signalling pathways. In the present study, we have tested whether the incorporation of n-3 PUFA in cardiac membrane phospholipids correlated with changes in the fatty acid composition of diacylglycerols (DAG) and led to a differential translocation of PKC isoforms. Two groups of five dogs were fed the standard diet supplemented with palm oil or fish oil for 8 weeks. Dogs fed a fish oil-enriched diet showed a preferential incorporation of EPA and, to a lesser extent, of DHA, at the expense of arachidonic acid, in the circulating TAG, plasma phospholipids, erythrocyte phospholipids and cardiomyocyte phospholipid fractions. Analysis of 1,2-DAG fatty acid composition also indicated a preferential enrichment of EPA compared with DHA. Associated with these results, a reduction in the expression of PKC-δ and PKC-ε isoforms in the particulate fractions was observed whereas no effect was seen for PKC-α and PKC-ζ. We conclude that a fish oil-enriched diet induces a modification in fatty acid composition of cardiac membrane phospholipids, associated with a differential translocation of PKC isoforms. These results can be explained by the production of structurally different DAG that may participate in some of the protective effects of n-3 PUFA against various chronic diseases.


2012 ◽  
Vol 41 (1) ◽  
pp. 86-93 ◽  
Author(s):  
T. Molnár ◽  
B. Kucska ◽  
A. Szabó ◽  
J. Biró ◽  
M. Bercsényi ◽  
...  

2014 ◽  
Vol 87 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Tamara B. Popović ◽  
Sunčica Z. Borozan ◽  
Marija M. Takić ◽  
Milica J. Kojadinović ◽  
Slavica Rankovic ◽  
...  

2011 ◽  
Vol 63 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Pérola Ribeiro ◽  
Fernanda Daniela Fernandes Carvalho ◽  
Amanda de Amaro Abreu ◽  
Marcela de Toledo Sant'Anna ◽  
Rosemary Joana de Lima ◽  
...  

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