scholarly journals Shewanella oneidensis MR-1 as a bacterial platform for electro-biotechnology

2021 ◽  
Author(s):  
Sota Ikeda ◽  
Yuki Takamatsu ◽  
Miyu Tsuchiya ◽  
Keigo Suga ◽  
Yugo Tanaka ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Shewanella Oneidensis ◽  
Value Added ◽  
Global Carbon Cycle ◽  
Future Directions ◽  
Redox States ◽  
Fundamental Knowledge ◽  
Electrochemically Active ◽  
Active Bacterium ◽  
Global Carbon

Abstract The genus Shewanella comprises over 70 species of heterotrophic bacteria with versatile respiratory capacities. Some of these bacteria are known to be pathogens of fishes and animals, while many are non-pathogens considered to play important roles in the global carbon cycle. A representative strain is Shewanella oneidensis MR-1 that has been intensively studied for its ability to respire diverse electron acceptors, such as oxygen, nitrate, sulfur compounds, metals, and organics. In addition, studies have been focused on its ability as an electrochemically active bacterium that is capable of discharging electrons to and receiving electrons from electrodes in bioelectrochemical systems (BESs) for balancing intracellular redox states. This ability is expected to be applied to electro-fermentation (EF) for producing value-added chemicals that conventional fermentation technologies are difficult to produce efficiently. Researchers are also attempting to utilize its electrochemical ability for controlling gene expression, for which electro-genetics (EG) has been coined. Here we review fundamental knowledge on this bacterium and discuss future directions of studies on its applications to electro-biotechnology (EB).

scholarly journals Carboxylases in Natural and Synthetic Microbial Pathways

2011 ◽  
Vol 77 (24) ◽  
pp. 8466-8477 ◽  
Author(s):  
Tobias J. Erb
Keyword(s):  
Carbon Dioxide ◽  
Synthetic Biology ◽  
Carbon Cycle ◽  
Greenhouse Gas ◽  
Inorganic Carbon ◽  
Carbon Assimilation ◽  
Value Added ◽  
Global Carbon Cycle ◽  
Global Carbon ◽  
Novel Antibiotics

ABSTRACTCarboxylases are among the most important enzymes in the biosphere, because they catalyze a key reaction in the global carbon cycle: the fixation of inorganic carbon (CO2). This minireview discusses the physiological roles of carboxylases in different microbial pathways that range from autotrophy, carbon assimilation, and anaplerosis to biosynthetic and redox-balancing functions. In addition, the current and possible future uses of carboxylation reactions in synthetic biology are discussed. Such uses include the possible transformation of the greenhouse gas carbon dioxide into value-added compounds and the production of novel antibiotics.

scholarly journals Diel Investments in Phytoplankton Metabolite Production Influenced by Associated Heterotrophic Bacteria

2020 ◽  
Author(s):  
Mario Uchimiya ◽  
William Schroer ◽  
Malin Olofsson ◽  
Arthur S. Edison ◽  
Mary Ann Moran
Keyword(s):  
Carbon Sequestration ◽  
Carbon Cycle ◽  
Organic Molecules ◽  
Heterotrophic Bacteria ◽  
Global Carbon Cycle ◽  
Phytoplankton Production ◽  
Surface Ocean ◽  
Extracellular Release ◽  
Carbon Transfer ◽  
Global Carbon

AbstractOrganic carbon transfer between photoautotrophic and heterotrophic microbes in the surface ocean mediated through metabolites dissolved in seawater is a central but poorly understood process in the global carbon cycle. In a synthetic microbial community in which diatom extracellular release of organic molecules sustained growth of a co-cultured bacterium, metabolite transfer was assessed over two diel cycles based on per cell quantification of phytoplankton endometabolites and bacterial transcripts. Of 31 phytoplankton endometabolites identified and classified into temporal abundance patterns, eight could be matched to patterns of bacterial transcripts mediating their uptake and catabolism. A model simulating the coupled endometabolite-transcription relationships hypothesized that one category of outcomes required an increase in phytoplankton metabolite synthesis in response to the presence of the bacterium. An experimental test of this hypothesis confirmed higher endometabolome accumulation in the presence of bacteria for all five compounds assigned to this category – leucine, glycerol-3-phosphate, glucose, and the organic sulfur compounds dihydroxypropanesulfonate and dimethylsulfoniopropionate. Partitioning of photosynthate into rapidly-cycling dissolved organic molecules at the expense of phytoplankton biomass production has implications for carbon sequestration in the deep ocean. That heterotrophic bacteria can impact this partitioning suggests a previously unrecognized influence on the ocean’s carbon reservoirs.Significance StatementMicrobes living in the surface ocean are critical players in the global carbon cycle, carrying out a particularly key role in the flux of carbon between the ocean and atmosphere. The release of metabolites by marine phytoplankton and their uptake by heterotrophic bacteria is one of the major routes of microbial carbon turnover. Yet the identity of these metabolites, their concentration in seawater, and the factors that affect their synthesis and release are poorly known. Here we provide experimental evidence that marine heterotrophic bacteria can affect phytoplankton production and extracellular release of metabolites. This microbial interaction has relevance for the partitioning of photosynthate between dissolved and particulate carbon reservoirs in the ocean, an important factor in oceanic carbon sequestration.

Are response function representations of the global carbon cycle ever interpretable?

Tellus B ◽  
2009 ◽  
Vol 61 (2) ◽  
Author(s):  
Sile Li ◽  
Andrew J. Jarvis ◽  
David T. Leedal
Keyword(s):  
Carbon Cycle ◽  
Response Function ◽  
Global Carbon Cycle ◽  
Global Carbon

The morphology of experimentally produced charcoal distinguishes fuel types in the Arctic tundra

The Holocene ◽  
2020 ◽  
Vol 30 (7) ◽  
pp. 1091-1096 ◽  
Author(s):  
Eleanor MB Pereboom ◽  
Richard S Vachula ◽  
Yongsong Huang ◽  
James Russell
Keyword(s):  
Arctic Tundra ◽  
Global Carbon Cycle ◽  
Fire Frequency ◽  
The Arctic ◽  
Fuel Type ◽  
The Past ◽  
Primary Means ◽  
Forested Ecosystems ◽  
Global Carbon ◽  
Tundra Ecosystems

Wildfires in the Arctic tundra have become increasingly frequent in recent years and have important implications for tundra ecosystems and for the global carbon cycle. Lake sediment–based records are the primary means of understanding the climatic influences on tundra fires. Sedimentary charcoal has been used to infer climate-driven changes in tundra fire frequency but thus far cannot differentiate characteristics of the vegetation burnt during fire events. In forested ecosystems, charcoal morphologies have been used to distinguish changes in fuel type consumed by wildfires of the past; however, no such approach has been developed for tundra ecosystems. We show experimentally that charcoal morphologies can be used to differentiate graminoid (mean = 6.77; standard deviation (SD) = 0.23) and shrub (mean = 2.42; SD = 1.86) biomass burnt in tundra fire records. This study is a first step needed to construct more nuanced tundra wildfire histories and to understand how wildfire will impact the region as vegetation and fire change in the future.

EPR-derived structures of flavin radical and iron-sulfur clusters from Methylosinus sporium 5 reductase

2021 ◽  
Author(s):  
Han Sol Jeong ◽  
Sugyeong Hong ◽  
Hee Seon Yoo ◽  
Jin Kim ◽  
Yujeong Kim ◽  
...  
Keyword(s):  
Carbon Cycle ◽  
Crucial Role ◽  
Methane Monooxygenase ◽  
Global Carbon Cycle ◽  
Ambient Conditions ◽  
Iron Sulfur Clusters ◽  
Iron Sulfur ◽  
Greenhouse Effects ◽  
Global Carbon ◽  
Significant Attention

Methane monooxygenase (MMO) has attracted significant attention owing to its crucial role in the global carbon cycle; it impedes greenhouse effects by converting methane to methanol under ambient conditions. The...

scholarly journals Amazonian Fire Events Disturbed the Global Carbon Cycle: A Study from 2019 Amazon Wildfire Using Google Earth Engine

2020 ◽  
Vol 3 (1) ◽  
pp. 43
Author(s):  
Subhajit Bandopadhyay ◽  
Dany A. Cotrina Sánchez
Keyword(s):  
Carbon Cycle ◽  
Carbon Sink ◽  
Brazilian Amazon ◽  
Global Carbon Cycle ◽  
Forest Productivity ◽  
Google Earth ◽  
Comparative Approach ◽  
Burned Area ◽  
Google Earth Engine ◽  
Global Carbon

An unprecedented number of wildfire events during 2019 throughout the Brazilian Amazon caught global attention, due to their massive extent and the associated loss in the Amazonian forest—an ecosystem on which the whole world depends. Such devastating wildfires in the Amazon has strongly hampered the global carbon cycle and significantly reduced forest productivity. In this study, we have quantified such loss of forest productivity in terms of gross primary productivity (GPP), applying a comparative approach using Google Earth Engine. A total of 12 wildfire spots have been identified based on the fire’s extension over the Brazilian Amazon, and we quantified the loss in productivity between 2018 and 2019. The Moderate Resolution Imaging Spectroradiometer (MODIS) GPP and MODIS burned area satellite imageries, with a revisit time of 8 days and 30 days, respectively, have been used for this study. We have observed that compared to 2018, the number of wildfire events increased during 2019. But such wildfire events did not hamper the natural annual trend of GPP of the Amazonian ecosystem. However, a significant drop in forest productivity in terms of GPP has been observed. Among all 11 observational sites were recorded with GPP loss, ranging from −18.88 gC m−2 yr−1 to −120.11 gC m−2 yr−1, except site number 3. Such drastic loss in GPP indicates that during 2019 fire events, all of these sites acted as carbon sources rather than carbon sink sites, which may hamper the global carbon cycle and terrestrial CO2 fluxes. Therefore, it is assumed that these findings will also fit for the other Amazonian wildfire sites, as well as for the tropical forest ecosystem as a whole. We hope this study will provide a significant contribution to global carbon cycle research, terrestrial ecosystem studies, sustainable forest management, and climate change in contemporary environmental sciences.

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