The EstA esterase is responsible for the main capacity of Lactococcus lactis to synthesize short chain fatty acid esters in vitro

2002 ◽  
Vol 93 (6) ◽  
pp. 994-1002 ◽  
Author(s):  
M. Nardi ◽  
C. Fiez-Vandal ◽  
P. Tailliez ◽  
V. Monnet
Keyword(s):  
Fatty Acid ◽  
Lactococcus Lactis ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Acid Esters

Biogeneration of Short Chain Fatty Acid Esters byPseudomonas FragiCRDA 037

1999 ◽  
Vol 17 (4) ◽  
pp. 269-282 ◽  
Author(s):  
Selim Kermasha ◽  
Barbara Bisakowski ◽  
André Morin ◽  
Safwan Ismail
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Acid Esters

scholarly journals Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

Metabolites ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 398 ◽  
Author(s):  
Siddabasave Gowda B. Gowda ◽  
Divyavani Gowda ◽  
Chongsheng Liang ◽  
Yonghan Li ◽  
Kentaro Kawakami ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Hydroxy Fatty Acids ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Rat Colon ◽  
Physiological Functions ◽  
Acid Esters

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.

Short-chain fatty acid transport and its effects on ion transport by rabbit cecum

1987 ◽  
Vol 253 (2) ◽  
pp. G171-G178
Author(s):  
M. Hatch
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Short Circuit ◽  
Basolateral Membrane ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Bathing Solution ◽  
Fatty Acid Transport ◽  
Unexpected Finding

Short-chain fatty acid (SCFA) metabolism and transport were examined in vitro across isolated rabbit cecal epithelia whose primary function is absorption of these solutes. This study shows that although there was some low-level metabolism of SCFAs to ketone bodies by the isolated cecum, a significantly higher oxygen consumption was sustained for a longer time period by tissues incubated in glucose-containing salines. The cecum supported a significant net secretory flux of acetate (J net Ac- = -1.13 +/- 0.13 mu eq X cm-2 X h-1) and propionate (J net Pr- = -0.61 +/- 0.14 mu eq X cm-2 X h-1). This study also shows that glucose significantly enhanced short-circuit current (Isc), tissue conductance (Gt), and sodium transport across this tissue. Neither Ac- nor Pr- enhanced net sodium flux (J net Ac-) but Pr- significantly reduced net chloride flux (J net Cl-), whereas Ac- had no effect. The increase in Isc and Gt observed in the presence of SCFAs was attributable to the presence of SCFA in the serosal bathing solution alone. To explain the latter finding and the unexpected finding of SCFA secretion, the existence of an electrogenic anion (HCO3-) secretory pathway is postulated. It is suggested that this system can accommodate SCFAs in vitro and that it is a Na+-dependent system located on the basolateral membrane of the cecal cell.

A Marine-Derived, Multi-mineral Supplement Influences Bacterial Fermentation and Short Chain Fatty Acid Profile In Vitro

2020 ◽  
Author(s):  
Valeria D. Felice ◽  
Denise M. O'Gorman ◽  
J. Apajalahti ◽  
T. Rinttilä ◽  
Nora M. O'Brien ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Mineral Supplement ◽  
Bacterial Fermentation
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