Intravenous feeding of the rat with short chain fatty acid esters. I. Glycerol monobutyrate

1977 ◽  
Vol 30 (12) ◽  
pp. 2078-2082 ◽  
Author(s):  
R H Birkhahn ◽  
R H McMenamy ◽  
J R Border
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Acid Esters

Biogeneration of Short Chain Fatty Acid Esters byPseudomonas FragiCRDA 037

1999 ◽  
Vol 17 (4) ◽  
pp. 269-282 ◽  
Author(s):  
Selim Kermasha ◽  
Barbara Bisakowski ◽  
André Morin ◽  
Safwan Ismail
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Acid Esters

scholarly journals Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

Metabolites ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 398 ◽  
Author(s):  
Siddabasave Gowda B. Gowda ◽  
Divyavani Gowda ◽  
Chongsheng Liang ◽  
Yonghan Li ◽  
Kentaro Kawakami ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Hydroxy Fatty Acids ◽  
Fatty Acid Esters ◽  
Short Chain ◽  
Rat Colon ◽  
Physiological Functions ◽  
Acid Esters

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.

scholarly journals Comprehensive evaluation for the one-pot biosynthesis of butyl-acetate by using microbial mono and co-cultures

2021 ◽  
Author(s):  
Yang Lv ◽  
Yujia Jiang ◽  
Jiasheng Lu ◽  
Hao Gao ◽  
Weiliang Dong ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Butyl Acetate ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Microbial Fermentation ◽  
Acetate Production ◽  
One Pot ◽  
The One ◽  
Acid Esters

Butyl acetate has shown wide attention in food, cosmetics, medicine and biofuel sectors. These short chain fatty acid esters can be produced by either chemical or biological synthetic process with corresponding alcohols and acids. Currently, biosynthesis of short chain fatty acid esters, such as butyl butyrate through microbial fermentation systems has been achieved; however, few studies regarding biosynthesis of butyl acetate were reported. In this study, three proof-of-principle strategies for the one-pot butyl acetate production from glucose by microbial fermentation was designed and evaluated. (1) 7.3 g/L of butyl acetate was synthesized by butanol producing Clostridium acetobutylicum NJ4 with the supplementation of exogenous acetic acid; (2) With the addition of butanol, 5.76 g/L of butyl acetate can be synthesized by acetate producing Actinobacillus succinogenes130z (ΔpflA) with the supplementation of exogenous butanol; (3) Microbial co-culture of C. acetobutylicum NJ4 and A. succinogenes130z (ΔpflA) can directly produce 2.2 g/L of butyl acetate from glucose, representing the first study in terms of butyl acetate production by using microbial co-culture system. Through the immobilization of A. succinogenes130z (ΔpflA), butyl acetate production was further improved to 2.86 g/L. These strategies may be extended to the biosynthesis of a wide range of esters, especially to some longer chain ones.

scholarly journals Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yang Lv ◽  
Yujia Jiang ◽  
Jiasheng Lu ◽  
Hao Gao ◽  
Weiliang Dong ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Butyl Acetate ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Fatty Acid Esters ◽  
Microbial Fermentation ◽  
Acetate Production ◽  
One Pot ◽  
The One ◽  
Acid Esters

Abstract Background Butyl acetate has shown wide applications in food, cosmetics, medicine, and biofuel sectors. These short-chain fatty acid esters can be produced by either chemical or biological synthetic process with corresponding alcohols and acids. Currently, biosynthesis of short chain fatty acid esters, such as butyl butyrate, through microbial fermentation systems has been achieved; however, few studies regarding biosynthesis of butyl acetate were reported. Results In this study, three proof-of-principle strategies for the one-pot butyl acetate production from glucose through microbial fermentation were designed and evaluated. (1) 7.3 g/L of butyl acetate was synthesized by butanol-producing Clostridium acetobutylicum NJ4 with the supplementation of exogenous acetic acid; (2) With the addition of butanol, 5.76 g/L of butyl acetate can be synthesized by acetate-producing Actinobacillus succinogenes130z (ΔpflA); (3) Microbial co-culture of C. acetobutylicum NJ4 and A. succinogenes130z (ΔpflA) can directly produce 2.2 g/L of butyl acetate from glucose by using microbial co-culture system with the elimination of precursors. Through the further immobilization of A. succinogenes130z (ΔpflA), butyl acetate production was improved to 2.86 g/L. Conclusion Different microbial mono- and co-culture systems for butyl acetate biosynthesis were successfully constructed. These strategies may be extended to the biosynthesis of a wide range of esters, especially to some longer chain ones.

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