Fitness of backcross six of hybrids between transgenic oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum)

2002 ◽  
Vol 11 (8) ◽  
pp. 1419-1426 ◽  
Author(s):  
G. Gueritaine ◽  
M. Sester ◽  
F. Eber ◽  
A. M. Chevre ◽  
H. Darmency
Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 469-472 ◽  
Author(s):  
A Benabdelmouna ◽  
G Guéritaine ◽  
M Abirached-Darmency ◽  
H Darmency

Genomic in situ hybridization (GISH) applied to the F1 interspecific hybrid between oilseed rape (Brassica napus, AACC, 2n = 38) and wild radish (Raphanus raphanistrum, RrRr, 2n = 18) showed the predicted 19 chromosomes from B. napus and 9 chromosomes from R. raphanistrum. The very low female fertility of these interspecific hybrids when backcrossed to R. raphanistrum led to only two descendants. Their chromosome number varied between 45 and 48. Both of these progenies showed only 9 chromosomes from R. raphanistrum and 36–39 chromosomes from B. napus. These results indicate the efficiency and limits of GISH as a suitable tool to assess and interpret the behavior of chromosomes after such interspecific crosses. The unexpected chromosome combination is discussed.Key words: genomic in situ hybridization, interspecific hybrid, introgression, oilseed rape, wild radish.


1993 ◽  
Vol 2 (6) ◽  
pp. 356-364 ◽  
Author(s):  
Jodi A. Scheffler ◽  
Russell Parkinson ◽  
Philip J. Dale

2009 ◽  
Vol 22 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Zheng Wang ◽  
Han Mao ◽  
Caihua Dong ◽  
Ruiqin Ji ◽  
Li Cai ◽  
...  

Sclerotinia sclerotiorum causes a highly destructive disease in oilseed rape (Brassica napus) resulting in significant economic losses. Studies on the Arabidopsis thaliana MPK4 loss-of-function mutant have implicated that AtMPK4 is involved in plant defense regulation, and its effect on disease resistance varies in different plant–pathogen interactions. In this study, we isolated a B. napus mitogen-activated protein kinase, BnMPK4, and found that BnMPK4 along with PDF1.2 are inducible in resistant line Zhongshuang9 but both are consistently suppressed in susceptible line 84039 after inoculation with S. sclerotiorum. Transgenic oilseed rape overexpressing BnMPK4 markedly enhances resistance to S. sclerotiorum and Botrytis cinerea. Further experiments showed that transgenic plants inhibited growth of S. sclerotiorum and constitutively activated PDF1.2 but decreased H2O2 production and constitutively suppressed PR-1 expression. Treatment of roots of the transgenic plants with H2O2 solution resulted in enhanced susceptibility to the two pathogens. Our results support the idea that MPK4 positively regulates jasmonic acid-mediated defense response, which might play an important role in resistance to S. sclerotiorum in oilseed rape.


2013 ◽  
Vol 21 (2) ◽  
pp. 1455-1465 ◽  
Author(s):  
Mirco Hecht ◽  
Bernadette Oehen ◽  
Jürg Schulze ◽  
Peter Brodmann ◽  
Claudia Bagutti

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