Outcrossing frequencies and distribution of transgenic oilseed rape (Brassica napus L.) in the nearest neighbourhood

Bromoxynil herbicide resistance is the newest type of broad-spectrum, non-selective herbicide resistance to be introduced into oilseed rape (Brassica napus L.). This herbicide resistance is conferred by a single transgene (the oxy gene), taken from a soil bacterium, which confers the ability to metabolize hydroxybenzonitrile herbicides such as bromoxynil. The level of resistance to bromoxynil herbicide in oilseed rape is high, but it is not known whether there are any performance changes associated with the oxy transgene or with the derived herbicide resistance. To determine if there are changes in performance related to the oxy transgene, or the derived herbicide resistance, two near-isogenic transgenic bromoxynil-resistant populations, with two different forms of the oxy gene, Westar 235 and Westar 237, were developed at the University of Manitoba, grown in the field in Manitoba for several years and evaluated for comparative performance. Westar 235 and Westar 237 near-isogenic populations, either sprayed with bromoxynil at 280 g a.i. ha–1 or left unsprayed, were compared with the non-transgenic near-isogenic population cultivar, Westar, in Winnipeg, Carman, and Portage la Prairie from 1994 to 1997. There were no consistent differences in the performance of the sprayed or unsprayed transgenic near-isogenic populations in comparison to Westar for any trait. The few significant and minimal differences that were found were evenly divided between positive effects and negative effects of the oxy gene and derived herbicide resistance. There were no significant effects of spraying bromoxynil herbicide detected in paired comparisons of Westar 235 and Westar 237 sprayed versus Westar 235 and Westar 237 unsprayed, respectively. The effects of the oxy transgenes and the effects of bromoxynil spraying of herbicide-resistant lines were negligible, indicating that there is little, if any biological cost associated with the bromoxynil resistance transgenes or related herbicide resistance. Key words: Transgenic oilseed rape, Brassica napus, biological cost

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RELEASE OF TRANSGENIC OILSEED RAPE (BRASSICA NAPUS L.) WITH ALTERED FATTY ACIDS

Acta Horticulturae ◽

10.17660/actahortic.1998.459.45 ◽

1998 ◽

pp. 379-388 ◽

Cited By ~ 4

Author(s):

E. Rudloff ◽

P. Wehling

Keyword(s):

Fatty Acids ◽

Brassica Napus ◽

Oilseed Rape ◽

Brassica Napus L ◽

Transgenic Oilseed Rape

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Resveratrol glucoside (Piceid) synthesis in seeds of transgenic oilseed rape (Brassica napus L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-005-0085-1 ◽

2005 ◽

Vol 111 (8) ◽

pp. 1553-1562 ◽

Cited By ~ 57

Author(s):

Alexandra Hüsken ◽

Alfred Baumert ◽

Carsten Milkowski ◽

Heiko C. Becker ◽

Dieter Strack ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Brassica Napus L ◽

Transgenic Oilseed Rape

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Seed Yield and Sulphur Partitioning in Two Inbred Lines of Low and High Glucosinolate Oilseed Rape (Brassica napus L.) and Their Hybrids at Three Levels of Sulphur Supply

Acta Agriculturae Scandinavica Section B - Soil & Plant Science ◽

10.1080/09064719908565565 ◽

1999 ◽

Vol 49 (3) ◽

pp. 184-188

Author(s):

J. Davik ◽

A. K. Bakken

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Seed Yield ◽

Inbred Lines ◽

Brassica Napus L

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Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

International Journal of Molecular Sciences ◽

10.3390/ijms21228740 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8740

Author(s):

Daria Chlebek ◽

Artur Pinski ◽

Joanna Żur ◽

Justyna Michalska ◽

Katarzyna Hupert-Kocurek

Keyword(s):

Brassica Napus ◽

Plant Growth ◽

Pseudomonas Fluorescens ◽

Oilseed Rape ◽

Plant Growth Promotion ◽

Fungal Pathogens ◽

Growth Promotion ◽

Genome Mining ◽

Plant Colonization ◽

Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

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