17 alpha-oestradiol and 17 beta-oestradiol do not affect basal and follicle-stimulating hormone-stimulated inhibin B secretion by highly purified rat Sertoli cells

Andrologia ◽  
2000 ◽  
Vol 32 (6) ◽  
pp. 345-349 ◽  
Author(s):  
J. Berlau ◽  
W. Roemer ◽  
M. Oettel ◽  
U. C. Hipler ◽  
V. Patchev ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Stimulating Hormone

scholarly journals SAT-035 In Vitro Effect of Different Follicle-Stimulating Hormone Preparations on Sertoli Cells

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Giuseppe Grande ◽  
Arato Iva ◽  
Ferran Barrachina ◽  
Catia Bellucci ◽  
Lilli Cinzia ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Pcr Analysis ◽  
A Chain ◽  
Main Regulator ◽  
And Function ◽  
Vitro Effect ◽  
Stimulating Hormone

Abstract Follicle-stimulating hormone (FSH) is the main regulator of spermatogenesis and plays a key role in the development and function of the reproductive system. To assess the effects of different FSH preparations in combination with testosterone on porcine pre-pubertal Sertoli cells, we performed Real Time PCR analysis of AMH, inhibin B and FSH-r, Western blotting analysis of AKT-posphoAKT, ERK1/2-posphoERK1/2, ELISA assay for AMH and inhibin B and a high-throughput proteomic analysis.We observed that all three preparations induced a reduction of AMH in terms of mRNA and secreted protein and, an increase of inhibin B in terms of mRNA in all the formulations while, only α-follitropin induced an increase of inhibin B secreted in the culture medium. Proteomic analysis permitted us to identify 46 secreted proteins.Of those, the SPARC protein was down-regulated after the treatment with testosterone associated with α-follitropin, β-follitropin and urofollitropin (vs group stimulated with T alone). 11 proteins were up-regulated by the different FSH preparations. In detail, Hemoglobin subunit beta, TPA and TPI have been observed to be up-regulated by stimulation with testosterone in addition with α-follitropin or with β-follitropin and or with urofollitropin. All preparations induced an increase in the secreted inhibin beta A chain, but in the medium after stimulation with urofollitropin we observed an higher increase in the levels of this protein. β-follitropin, associated with testosterone, specifically induces an up-regulation of 8 specific secreted proteins.Our study, showing that the three FSH preparations were associated with different effects, could offer the opportunity to shed light inside applications to personalized reproductive medicine.

scholarly journals The IGF1 Receptor Is Involved in Follicle-Stimulating Hormone Signaling in Porcine Neonatal Sertoli Cells

2019 ◽  
Vol 8 (5) ◽  
pp. 577 ◽  
Author(s):  
Rossella Cannarella ◽  
Iva Arato ◽  
Rosita A. Condorelli ◽  
Giovanni Luca ◽  
Federica Barbagallo ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Testicular Development ◽  
Mrna Levels ◽  
Mediated Effects ◽  
Igf1r Inhibitor ◽  
Pre Treatment ◽  
Igf1 Receptor ◽  
Stimulating Hormone

Experimental evidence has shown that the IGF1 receptor (IGF1R) is involved in testicular development during embryogenesis. More recently, data gathered from mice granulosa cells and zebrafish spermatogonia suggest that IGF1R has a role in Follicle-stimulating hormone (FSH) signaling. No evidence has been reported on this matter in Sertoli cells (SCs) so far. The aim of the study was to evaluate the role, if any, of the IGF1R in FSH signaling in SCs. The effects of FSH exposure on myosin-phosphatase 1 (MYPT1), ERK 1/2, AKT308, AKT473, c-Jun N-terminal kinase (JNK) phosphorylation and on anti-Müllerian hormone (AMH), inhibin B and FSH receptor (FSHR) mRNA levels were assessed with and without the IGF1R inhibitor NVP-AEW541 in purified and functional porcine neonatal SCs. Pre-treatment with NVP-AEW541 inhibited the FSH-induced MYPT1 and ERK 1/2 phosphorylation, decreased the FSH-dependent Protein kinase B (AKT)308 phosphorylation, but did not affect the FSH-induced AKT473 and JNK phosphorylation rate. It also interfered with the FSH-induced AMH and FSHR down-regulation. No influence was observed on the FSH-stimulated Inhibin B gene expression. Conclusion. These findings support the role of theIGF1R in FSH signaling in porcine SCs. The possible influence of IGF1 stimulation on the FSH-mediated effects on SCs should be further explored.

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

236. Effects of long term recombinant rat follicle-stimulating hormone replacement on the restoration of spermatogenesis after chronic suppression of gonadotrophins in adult rats

2008 ◽  
Vol 20 (9) ◽  
pp. 36
Author(s):  
S. M. Ruwanpura ◽  
P. G. Stanton ◽  
D. M. Robertson ◽  
R. I. McLachlan ◽  
Y. Makanji ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Germ Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Testis Weight ◽  
Adult Rats ◽  
Early Pachytene ◽  
Pachytene Spermatocytes ◽  
Stimulating Hormone

Follicle stimulating hormone (FSH) in short-term rat studies supports spermatogenesis at multiple levels, notably spermatogonial development. The role of FSH in supporting full spermatogenesis in rats is still in question as long-term studies have not been possible due the development of neutralising antibodies to heterologous FSH preparations. This study sought to assess the effects of a homologous recombinant rat FSH (rr-FSH) preparation on the long-term restoration of spermatogenesis. Adult rats were GnRH-immunised (GnRH-im) for 12 weeks then, administered an anti-androgen; flutamide (flut), alone or together with rr-FSH (8µg/rat/daily) for 56 days (1 spermatogenic cycle). Germ and Sertoli cell numbers were quantified using an optical disector stereological method. Testis weight, serum FSH and inhibin B and Sertoli cell nuclear volume were significantly reduced to 15%, 13%, 25% and 57% of controls respectively, following GnRH-im+flut treatment. GnRH-im+flut treatment reduced A/I spermatogonial, type B spermatogonial+preleptotene, leptotene+zygotene and early pachytene spermatocyte numbers to 28%, 68%, 50% and 19% (P < 0.001) of controls respectively, with later germ cells rarely observed. After FSH treatment, no significant affect on testis weight, serum FSH and inhibin B or Sertoli cell number were observed. However, rr-FSH treatment significantly increased numbers of A/I spermatogonia, leptotene+zygotene and early pachytene spermatocytes from 28 = >42%, 50 = >69% and 19 = >27% of controls, respectively, while no differences were observed in later germ cell types. rr-FSH also increased (P < 0.05) the volume of Sertoli cell nuclei from 57 = >66% of control. In conclusion, FSH is unable to support full rat spermatogenesis; however, FSH can partially support germ cells notably spermatogonia through to early pachytene spermatocytes, despite the absence of androgenic support.

scholarly journals Comparison of Serum Inhibin B and Follicle-Stimulating Hormone (FSH) Level between Normal and Infertile Men in Yaoundé

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Tchoula Mamiafo Corinne ◽  
Pieme Constant Anatole ◽  
Ngogang Yonkeu Jeanne
Keyword(s):  
Semen Quality ◽  
Follicle Stimulating Hormone ◽  
Sperm Concentration ◽  
Reproductive Hormones ◽  
Inhibin B ◽  
World Health ◽  
Infertile Couple ◽  
Male Reproductive Function ◽  
Significant Difference ◽  
Stimulating Hormone

Objective. Hormones play a vital role in initiating and maintaining male reproductive function. The present study explores the influence and predictive ability of two reproductive hormones on semen quality among men who were partners in an infertile couple. Design. During our cross sectional study, men were recruited from private and public hospital and laboratories for clinical evaluation of fertility status. Methods. Fresh semen samples were assessed for quality (concentration, motility and morphology) according to the 2010 World Health Organization manual and the serum levels of hormones, including follicle-stimulating hormone (FSH), Inhibin B was measured (ELISA). Results. We found a significant difference in the two groups regarding sperm concentration (p<0.0001), total sperm count (p<0.0001), progressive motility (p<0.0001), vitality (p<0.0001) and the percentage of normal forms (p=0.043). We found a strong and negative correlation between FSH and Inhibin B in the overall population, the normozoospermic reference group and the case group. Our study confirmed that Inhibin B was significantly and positively correlated with sperm concentration and leucocytes, and that FSH was negatively correlated to sperm concentration and vitality. Conclusion. Consistent with other studies, our results show strong association between semen quality and FSH and Inhibin B.

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