Influence of the culture medium on antibiotic susceptibility testing of food-associated lactic acid bacteria with the agar overlay disc diffusion method

ABSTRACT Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Lactococcus, and Bifidobacterium. A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with l-cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.

Download Full-text

Stenotrophomonas maltophilia endogenous endophthalmitis: clinical presentation, sensitivity spectrum and management

Journal of Medical Microbiology ◽

10.1099/jmm.0.009431-0 ◽

2009 ◽

Vol 58 (6) ◽

pp. 837-838 ◽

Cited By ~ 8

Author(s):

T. Das ◽

H. S. Deshmukh ◽

A. Mathai ◽

Ashok Kumar Reddy

Keyword(s):

Stenotrophomonas Maltophilia ◽

Susceptibility Testing ◽

Clinical Presentation ◽

Diffusion Method ◽

Endogenous Endophthalmitis ◽

Antibiotic Susceptibility Testing ◽

Disc Diffusion ◽

Antibiotic Regimen ◽

Nosocomial Pathogen ◽

Disc Diffusion Method

Stenotrophomonas maltophilia is gaining importance as a community-acquired pathogen, after becoming firmly established as a nosocomial pathogen. Here we report a case of endogenous endophthalmitis due to S. maltophilia. Antibiotic-susceptibility testing of the isolate was performed by the Kirby–Bauer disc diffusion method. The organism was resistant to aminoglycosides, imipenem, ticarcillin and cotrimoxazole and was sensitive to ceftazidime and chloramphenicol. The patient was successfully treated with a sensitivity-based intravitreal antibiotic regimen.

Download Full-text

Isolasi Bakteri Asam Laktat dari Usus Sapi (Bos taurus) serta Kemampuannya dalam Menghambat Pertumbuhan Bakteri Eschericia coli dan Shigella sp

Jurnal Ilmiah Biologi UMA (JIBIOMA) ◽

10.31289/jibioma.v1i1.145 ◽

2019 ◽

Vol 1 (1) ◽

pp. 21-27

Author(s):

Lulu Fatma Dewi ◽

Sartini Sartini ◽

Rahmiati Rahmiati

Keyword(s):

Escherichia Coli ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Bos Taurus ◽

Inhibition Zone ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Positive Results ◽

Paper Disc

The purpose of this research was to determine the ability of BAL from cattle intestine (Bos taurus) in inhibiting the growth of Eschericia coli and Shigella sp. Testing of BAL capability in inhibiting growth of Escherichia coli and Shigella sp. using the disc-diffusion method or the Kirby-Bauer method, which was done by measuring the inhibit zone around the paper disc. Data were analyzed descriptively by displaying data in table and picture form. The results obtained 2 isolates of BAL from the cow intestine. All isolates showed positive results when tested for antibacterial against Escherichia coli and Shigella sp. In isolate BAL with code sp1 has inhibition zone against Escherichia coli equal to 7.5 mm and to Shigella sp. of 6.8 mm, whereas in isolate BAL with code sp2 has inhibition zone against Escherichia coli equal to 8.9 mm and to Shigella sp. of 8.0 mm. Based on the results obtained, it can be concluded that isolates of lactic acid bacteria with sp2 code has inhibition zone 8.9 mm in inhibiting Eschericia coli while against bacteria Shigella sp. has a diameter of 8.0 mm.

Download Full-text

Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp.

Jurnal Ilmiah Biologi UMA (JIBIOMA) ◽

10.31289/jibioma.v2i1.185 ◽

2020 ◽

Vol 2 (1) ◽

pp. 23-31

Author(s):

Alpina Bukhori ◽

Sartini Sartini ◽

Rahmiati Rahmiati

Keyword(s):

Staphylococcus Aureus ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Digestive Tract ◽

Oreochromis Niloticus ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Isolation And Characterization ◽

Inhibitory Power

Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.

Download Full-text

ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v5i1.1707 ◽

2018 ◽

Vol 5 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Edy Fachrial ◽

Harmileni Harmileni

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Palm Oil ◽

Pathogenic Bacteria ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method

<p><em>The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.</em></p>

Download Full-text

Russia-made Mueller–Hinton agar: compliance with contemporary requirements

Russian Journal of Infection and Immunity ◽

10.15789/2220-7619-2019-2-409-416 ◽

2019 ◽

Vol 9 (2) ◽

pp. 409-416

Author(s):

L. V. Domotenko ◽

I. S. Kosilova ◽

A. P. Shepelin

Keyword(s):

Quality Control ◽

Antimicrobial Agents ◽

Susceptibility Testing ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Nutrient Media ◽

Mueller Hinton ◽

Mueller Hinton Agar

At present, a rise of antimicrobial resistance requires that susceptibility of infectious agents to antimicrobial agents could be accurately evaluated as related errors may lead to selecting improper therapeutics provoking spread of drug resistance. Pathogen sensitivity to antimicrobial agents is commonly determined by a disc diffusion method. A quality of nutrient medium used in assays plays a crucial role influencing final results. In Russia, it turned out that regulatory documents such as the nationwide guidelines and clinical recommendations outlining methodology for antimicrobial susceptibility testing underlay availability in domestic market few nutrient media, including Mueller–Hinton Agar, AGV medium etc. exhibiting sometimes unsatisfactory quality. To harmonize such methodology with international requirements, theStateResearchCenterfor Applied Microbiology and Biotechnology has developed a technology and promoted manufacture of Russia-made Mueller–Hinton agar satisfying requirements of EUCAST documents, clinical guidelines, and ISO/TS 16782:2016. The main objective of this study was to compare quality of new agar product with five similar foreign media while examining 11 test strains by disc diffusion method. As a result, some of nutrient media available to the Russian market turned out to be off-standard: not all of them satisfy to the EUCAST requirements and clinical guidelines since diameter distribution for growth inhibition recommended by EUCAST for quality control does not fit into permissible range. Moreover, susceptibility of P. aeruginosa ATCC 27853 to aminoglycosides, fluoroquinolones, Meropenem, as well as S. aureus ATSS 25923 and E. faecalis ATCC 29212 to tigecycline was assessed with certain mistakes. The data obtained by us were analyzed in accordance to the new document ISO/TS 16782:2016 “Clinical laboratory testing — criterion for acceptable lots of dehydrated Mueller–Hinton agar and broth for antimicrobial susceptibility testing”, not approved yet In Russia. To determine potential reason for deviation of data from reference range, we measured concentration of bivalent metals in all nutrient media examined by atomic emission spectrometry with inductively coupled plasma. We determined new patterns affecting reliability of results on microbial antibiotic susceptibility. A need to check intralaboratory quality control of nutrient media was emphasized.

Download Full-text

Improvement of a disk diffusion method for antibiotic susceptibility testing of anaerobic bacteria. French recommendations revisited for 2020

Anaerobe ◽

10.1016/j.anaerobe.2020.102213 ◽

2020 ◽

Vol 64 ◽

pp. 102213

Author(s):

L. Dubreuil ◽

F. Jehl ◽

C. Cattoen ◽

R. Bonnet ◽

J.P. Bru ◽

...

Keyword(s):

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Anaerobic Bacteria ◽

Disk Diffusion ◽

Diffusion Method ◽

Antibiotic Susceptibility Testing ◽

Disk Diffusion Method

Download Full-text

In Vitro Evaluation of Antifungal Activity of Three Traditionally Used Medicinal Plants; Umbilicus Intermedius Boiss, Cuminum Cyminum and Zingiber Officinale Extracts

International Journal of Medical Laboratory ◽

10.18502/ijml.v6i4.2006 ◽

2019 ◽

Author(s):

Ameneh Takesh ◽

Mahnaz Fatahinia ◽

Ali Zarei Mahmoudabadi

Keyword(s):

Antifungal Activity ◽

Culture Medium ◽

Zingiber Officinale ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Cuminum Cyminum ◽

Medicinal Plant Extracts ◽

Alternaria Sp ◽

Medium Method

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods. Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium spp., Mucor spp., Stemphylium spp., Drechslera spp., Alternaria spp., Cladosporium spp., and Aureobasidium pullulans. Furthermore, 17 candida isolates including, C. albicans, C. glabrata and C. dubliniensis were tested. In the present study two methods of disc diffusion method, agar wells diffusion method were used for assay. Then, the mixing with culture medium method was used for assessment of the antifungal activity of extracts against Alternaria sp.(as black mold), A. terreus (as hyaline mold) and C. albicans (as yeast) to compare methods as well. Results: No fungi were susceptible to extracts in disc diffusion method and agar wells diffusion method. But, this study showed that in mixing with culture medium method, cumin extract has valuable anti-fungal property and Umbilicus intermedius boiss has the inhibitory properties against the black fungi. Furthermore, it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods. Alternaria sp. and C. albicans were susceptible and resistant to all extracts. Conclusions: it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods and inhibitory potency of the extracts varies according to the type of extraction and their concentration.

Download Full-text