In Vitro Evaluation of Antifungal Activity of Three Traditionally Used Medicinal Plants; Umbilicus Intermedius Boiss, Cuminum Cyminum and Zingiber Officinale Extracts

2019 ◽  
Author(s):  
Ameneh Takesh ◽  
Mahnaz Fatahinia ◽  
Ali Zarei Mahmoudabadi
Keyword(s):  
Antifungal Activity ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Cuminum Cyminum ◽  
Medicinal Plant Extracts ◽  
Alternaria Sp ◽  
Medium Method

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods.  Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium spp., Mucor spp., Stemphylium spp., Drechslera spp., Alternaria spp., Cladosporium spp., and Aureobasidium pullulans. Furthermore, 17 candida isolates including, C. albicans, C. glabrata and C. dubliniensis were tested. In the present study two methods of disc diffusion method, agar wells diffusion method were used for assay. Then, the mixing with culture medium method was used for assessment of the antifungal activity of extracts against Alternaria sp.(as black mold), A. terreus (as hyaline mold) and C. albicans (as yeast) to compare methods as well. Results: No fungi were susceptible to extracts in disc diffusion method and agar wells diffusion method. But, this study showed that in mixing with culture medium method, cumin extract has valuable anti-fungal property and Umbilicus intermedius boiss has the inhibitory properties against the black fungi. Furthermore, it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods. Alternaria sp. and C. albicans were susceptible and resistant to all extracts.  Conclusions: it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods and inhibitory potency of the extracts varies according to the type of extraction and their concentration.

The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

10.5219/1695 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1112-1119
Author(s):  
Hana Ďúranová ◽  
Veronika Valková ◽  
Lucia Galovičová ◽  
Jana Štefániková ◽  
Miroslava Kačániová
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Vapor Phase ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Wheat Bread ◽  
Citrus Reticulata ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

scholarly journals IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS

2020 ◽  
pp. 39-42
Author(s):  
SWETA KUMARI ◽  
VIRENDRA VAISHNAV
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
High Efficiency ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Cinnamomum Tamala ◽  
Medicinal Plant Extracts

Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli. Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC). Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria. Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.

scholarly journals ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS

2017 ◽  
Vol 9 (7) ◽  
pp. 140
Author(s):  
Anto Suganya Regis ◽  
Jeya Jothi Gabriel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
High Concentration ◽  
Disc Diffusion Method ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Methanol Extracts

Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential extraction method using solvents hexane, ethyl acetate and methanol based on their polarity. The antifungal activity of the extracts was tested against 12 opportunistic fungal strains by disc diffusion method. Minimum inhibitory concentration (MIC) was determined using microtiter plate method.Results: The hexane, ethyl acetate and methanol extracts showed significant antifungal activities. The highest antifungal activity was recorded for ethyl acetate extract of C. axillaris. In disc diffusion method at high concentration (5 mg/ml), the ethyl acetate extract exhibited the zone of inhibition>30 mm against C. krusei, mentagrophytes, Scopulariopsis sp. and B. cinerea. In MIC the ethyl acetate extract inhibited the growth of T. mentagrophytes, Scopulariopsis sp., B. cinerea in its low dose (0.031 mg/ml). The hexane, ethyl acetate and methanol extracts of C. axillaris did not show activity against M. gypseum, T. rubrum and E. floccosum.Conclusion: This is the first report for the antifungal efficacy of C. axillaris. The results proved that the extracts of C. axillaris have high potential antifungal principles which could fight against the opportunistic and multidrug resistant fungal strains.

scholarly journals Antifungal Activity of TiO2/Ag Nanoparticles under Visible Light Irradiation

2020 ◽  
Vol 21 (1) ◽  
pp. 14
Author(s):  
Nahzim Rahmat ◽  
Endang Tri Wahyuni ◽  
Adhitasari Suratman
Keyword(s):  
Antifungal Activity ◽  
Visible Light ◽  
Visible Light Irradiation ◽  
Ag Nanoparticles ◽  
Irradiation Time ◽  
Light Irradiation ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Research Results

The doping of TiO2 by Ag(I) from [Ag(S2O3)2]3– contained in radiophotography wastewater by photoreduction method has been performed. TiO2/AgNPs photocatalyst was examined for its activity as an antifungal material for the inhibition of C. albicans in water under visible light irradiation. In the doping process, the weight of TiO2 was varied to obtain TiO2/AgNPs with different amounts of Ag. The TiO2/AgNPs samples were characterized by using FTIR, SRUV, TEM, SEM-EDX, and XRD methods. The antifungal test was carried out by disc diffusion method under visible light irradiation, wherein the amount of Ag-doped on TiO2, the dose of TiO2/AgNPs, and the irradiation time were optimized. The research results indicated that the antifungal activity of TiO2/AgNPs in inhibiting C. albicans has been successfully prepared. The highest inhibition was achieved by using 0.5 g/L of TiO2/AgNPs (I), at 5 h of irradiation time.

scholarly journals Anti-fungal potential of tridhara (Tridax procumbens) leaves

2016 ◽  
Vol 1 (3) ◽  
pp. 686-689 ◽  
Author(s):  
Md Faruk Miya ◽  
Zakirul Islam ◽  
Sayeed Shahriyar ◽  
Md Riad Hossain Khan ◽  
Md Salim Reza
Keyword(s):  
Antifungal Activity ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Current Investigation ◽  
Disc Diffusion Method ◽  
Fungal Strains ◽  
Methanolic Extracts ◽  
Tridax Procumbens ◽  
Effective Inhibition ◽  
Anti Fungal

The antifungal activity of methanolic extracts of leaves of Tridax procumbens was studied. Fungal strains Aspergillus niger and Aspergillus ocraceous were selected for the study. Disc diffusion method was applied against the selected fungal strains and compared to the drug ciprofloxacin to observe the antifungal activity of the methanolic extracts of Tridax procumbens leaves. The present study demonstrated that the disks of methanolic extracts of T. procumbens showed effective inhibition against A. niger and A. ocraceous compared to ciprofloxacin. The results of the current investigation suggest that the methanolic extracts of T. procumbens leaves can be used for the treatment of the diseases caused by the tested organism and it has significant scope to develop a novel broad spectrum of antifungal herbal formulations.Asian J. Med. Biol. Res. December 2015, 1(3): 686-689

AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 12-19
Author(s):  
Oom Komala ◽  
Yulianita . ◽  
Fuji Raka Siwi
Keyword(s):  
Antifungal Activity ◽  
Ethanol Extract ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Lawsonia Inermis ◽  
Disc Diffusion Method ◽  
Mic Value

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.

scholarly journals ANTIFUNGAL ACTIVITY OF KOMBUCHA TEA AGAINST HUMAN PATHOGENIC FUNGI

2016 ◽  
Vol 9 (5) ◽  
pp. 253 ◽  
Author(s):  
Ari - Yuniarto ◽  
Kusnandar Anggadiredja ◽  
Riana Annisa Nur Aqidah
Keyword(s):  
Antifungal Activity ◽  
Infectious Diseases ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Microsporum Gypseum ◽  
Major Contributor ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Kombucha Tea ◽  
Bacteria And Fungi

ABSTRACTObjective: In general, infectious diseases still known as a major contributor to the number of morbidity and mortality in the worldwide besideother diseases. Infections are ordinarily caused by viruses, bacteria, and fungi. Several human pathogenic fungi involving Aspergillus flavus, Candidaalbicans, and Microsporum gypseum have responsible against infectious diseases. The objective of the research was to evaluate the antifungal activityof kombucha tea against human pathogenic fungi such as A. flavus, C. albicans, and M. gypseum.Methods: The antifungal activity of the kombucha tea against A. flavus, C. albicans, and M. gypseum were tested by disc diffusion method with durationof fermentation in 6, 12, and 18 days.Results: Based on the present study, inhibitory diameter of A. flavus, C. albicans, and M. gypseum are 16.83, 15.36, and 25.06 mm, respectively. Theinhibitory diameter was obtained from kombucha tea activity with duration of fermentation in 6 days.Conclusion: The results provide support for the use of kombucha tea as a potential antifungal source against human pathogenic fungi involvingA. flavus, C. albicans, and M. gypseum.Keywords: Antifungal, Kombucha tea, Pathogenic, Fungi.

scholarly journals What about the Antibacterial Activity of Fresh Ginger Juice (Zingiber officinale)?

2021 ◽  
pp. 163-172
Author(s):  
E. Günaydın ◽  
Gülşen Goncagül ◽  
Çiğdem Güceyü ◽  
Ö. Kardoğan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Intensive Care ◽  
Antibacterial Effect ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial Effects ◽  
Disc Diffusion Method

Background: The aim of this study was to determine the antibacterial activity of the juice obtained by squeezing fresh ginger roots (Zingiber officinale) against the standard strains Staphylococcus aureus (ATCC® 25923) and Escherichia coli (ATCC® 25922), as well as Acinetobacter baumannii and Klebsiella pneumoniae isolated from patients hospitalized in intensive care units. Methods: For this purpose, the antibacterial effect of the fresh ginger juice was tested against antibiotics such as ampicillin, cefazolin, cefuroxime, meropenem, colistin, ofloxacin, sulfamethoxazole/trimethoprim, tetracycline, and gentamicin. The antibacterial effects against the tested bacteria were determined by the disc diffusion method using 5 µL, 10 µL, 15 µL and 20 µL amounts of ginger juice impregnated on sterile discs. Results: As a result of the study, it was detected that the antibacterial effect increased in parallel with the increasing amounts of fresh ginger juice in all tested bacteria. Conclusion: Consequently, fresh Zingiber officinale can provide protection to a certain extent against bacterial pathogens due to its antibacterial action.

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