scholarly journals Differential diagnosis between normal endometrium and endometrial hyperplasia with immunostaining cytology using anti-LeY monoclonal antibody

2003 ◽  
Vol 13 (1) ◽  
pp. 42-46 ◽  
Keyword(s):  
Monoclonal Antibody ◽  
Differential Diagnosis ◽  
Endometrial Hyperplasia ◽  
Normal Endometrium

scholarly journals Differential diagnosis between normal endometrium and endometrial hyperplasia with immunostaining cytology using anti-LeY monoclonal antibody

2003 ◽  
Vol 13 (1) ◽  
pp. 42-46
Author(s):  
Y. Arai ◽  
M. Nishida
Keyword(s):  
Monoclonal Antibody ◽  
Differential Diagnosis ◽  
Endometrial Cancer ◽  
Endometrial Carcinoma ◽  
Endometrial Hyperplasia ◽  
Negative Staining ◽  
Positive Staining ◽  
Normal Endometrium ◽  
Number Of Cells

We have previously reported that both endometrial cancer and endometrial hyperplasia stain positively for the anti-LeY monoclonal antibody, whereas normal endometrium does not. Endometrial hyperplasia is a premalignant change associated with the eventual development of endometrial carcinoma. However, it can be difficult to differentiate hyperplasia from normal endometrium in cytology. This study illustrates the use of immunocytochemical cytology using anti-LeY monoclonal antibody to differentiate between endometrial hyperplasia and normal endometrium. Immunostaining using anti-LeY monoclonal antibody was performed on cytologic specimens obtained from 17 normal endometria, 25 endometria with endometrial hyperplasia, and 13 endometria with endometrial carcinoma. All normal endometria displayed negative staining for anti-LeY monoclonal antibody, whereas all endometria with endometrial carcinoma displayed positive staining. Of the endometrial hyperplasia cases, 21 displayed positive staining. However, four displayed negative staining due to the small number of cells available for diagnosis. We believe that immunostaining cytology using anti-LeY monoclonal antibody is a useful method for differentiating between normal endometrium and endometrial hyperplasia.

scholarly journals Loss of ten-eleven translocation 1 (TET1) expression as a diagnostic and prognostic biomarker of endometrial carcinoma

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259330
Author(s):  
Nien-Tzu Liu ◽  
Cherng-Lih Perng ◽  
Yu-Ching Chou ◽  
Pi-Shao Ko ◽  
Yi-Jia Lin ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Endometrial Carcinoma ◽  
Endometrial Hyperplasia ◽  
Expression Profiles ◽  
Gynecological Cancer ◽  
Dna Demethylation ◽  
Atypical Hyperplasia ◽  
Mrna Levels ◽  
Normal Endometrium ◽  
Expression Score

Endometrial carcinoma (EC) is the most common gynecological cancer. However, there is currently no routinely used biomarker for differential diagnosis of malignant and premalignant endometrial lesions. Ten-eleven translocation (TET) proteins, especially TET1, were found to play a significant role in DNA demethylation, via conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC). TET1, 5-mC, and 5-hmC expression profiles in endometrial carcinogenesis are currently unclear. We conducted a hospital-based retrospective review of the immunohistochemical expression of TET1, 5-mC, and 5-hmC in 181 endometrial samples. A “high” TET1 and 5-hmC expression score was observed in all cases of normal endometrium (100.0% and 100.0%, respectively) and in most samples of endometrial hyperplasia without atypia (90.9% and 78.8%, respectively) and atypical hyperplasia (90.6% and 93.8%, respectively), but a “high” score was found in only less than half of the EC samples (48.8% and 46.5%, respectively). The TET1 and 5-hmC expression scores were significantly higher in normal endometrium and premalignant endometrial lesions than in ECs (p < 0.001). A “high” 5-mC expression score was observed more frequently for ECs (81.4%) than for normal endometrium (40.0%), endometrial hyperplasia without atypia (51.5%), and atypical hyperplasia (53.1%) (p < 0.001). We also found that TET1 mRNA expression was lower in ECs compared to normal tissues (p = 0.0037). TET1 immunohistochemistry (IHC) scores were highly proportional to the TET1 mRNA levels and we summarize that the TET1 IHC scoring can be used for biomarker determinations. Most importantly, a higher TET1 score in EC cases was associated with a good overall survival (OS) rate, with a hazard ratio (HR) of 0.31 for death (95% confidence interval: 0.11–0.84). Our findings suggest that TET1, 5-mC, and 5-hmC expression is a potential histopathology biomarker for the differential diagnosis of malignant and premalignant endometrial lesions. TET1 is also a potential prognostic marker for EC.

scholarly journals Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2

2020 ◽  
Vol 10 (2) ◽  
pp. 157-163
Author(s):  
María Carla García Mitacek ◽  
Romina Gisele Praderio ◽  
María Cecilia Stornelli ◽  
Rodolfo Luzbel De la Sota ◽  
María Alejandra Stornelli
Keyword(s):  
Stromal Cells ◽  
Endometrial Hyperplasia ◽  
Inflammatory Cells ◽  
Immunohistochemical Localization ◽  
Cyclooxygenase 2 ◽  
Immunohistochemical Expression ◽  
Normal Endometrium ◽  
Pathophysiological Mechanisms ◽  
Mixed Breed ◽  
Strong Staining

Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known.Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis.Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS.Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003).Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required. Keywords: Bitch, COX2, Endometritis, Immunohistochemical.

scholarly journals PAX2 in endometrial carcinogenesis and in differential diagnosis of endometrial hyperplasia: A systematic review and meta-analysis of diagnostic accuracy

2019 ◽  
Vol 98 (3) ◽  
pp. 287-299 ◽  
Author(s):  
Antonio Raffone ◽  
Antonio Travaglino ◽  
Gabriele Saccone ◽  
Massimo Mascolo ◽  
Luigi Insabato ◽  
...  
Keyword(s):  
Systematic Review ◽  
Differential Diagnosis ◽  
Diagnostic Accuracy ◽  
Endometrial Hyperplasia ◽  
Meta Analysis

BAG-1 expression in normal endometrium, endometrial hyperplasia and endometrial cancer

2008 ◽  
Vol 87 (8) ◽  
pp. 862-867 ◽  
Author(s):  
Jae Yun Song ◽  
Ji Won Kim ◽  
Jae Kwan Lee ◽  
Nak Woo Lee ◽  
Bom Woo Yeom ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Endometrial Hyperplasia ◽  
Normal Endometrium

scholarly journals Characterization of cell clusters in smears from normal endometrium, endometrial hyperplasia and well differentiated adenocarcinoma.

1995 ◽  
Vol 34 (3) ◽  
pp. 439-448 ◽  
Author(s):  
Yoshiaki NORIMATSU ◽  
Hiromi KODA ◽  
Shuji HAMAZAKI ◽  
Yuri OZEKI ◽  
Kyoumi NAKAKUNI ◽  
...  
Keyword(s):  
Endometrial Hyperplasia ◽  
Normal Endometrium ◽  
Cell Clusters ◽  
Well Differentiated

Interleukin 6 in Cerebrospinal Fluid of Patients with Meningitis is not a Useful Diagnostic Marker in the Differential Diagnosis of Meningitis

1997 ◽  
Vol 34 (2) ◽  
pp. 165-169 ◽  
Author(s):  
L F López-Cortés ◽  
M Cruz-Ruiz ◽  
J Gómez-Mateos ◽  
D Jiménez-Hernández ◽  
P Viciana-Fernández ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Monoclonal Antibody ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Differential Diagnosis ◽  
Interleukin 6 ◽  
Diagnostic Marker ◽  
Neurological Diseases ◽  
Central Nervous System Infections ◽  
Pyogenic Meningitis

We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples classified as pyogenic (15), viral (15), self-resolving aseptic meningitis (20), other infectious meningitis (9), neoplastic (4) and normal CSF from patients with (20) and without (15) non-infectious neurological diseases. CSF IL-6 concentrations were increased in pyogenic meningitis (100%) and in more than 50% of viral and other subarachnoid space infections, and rarely in patients without central nervous system infections. Though patients affected by pyogenic meningitis showed the highest levels of CSF IL-6, only a cut-off point ≥10000 pg/mL was able to discriminate pyogenic meningitis from those of other aetiologies with a specificity ≥94% and a positive predictive value of ≥0·75 but the sensitivity was ≤60%. Therefore, CSF IL-6 concentration is not a good diagnostic marker in the differential diagnosis of meningitis.

Human telomerase RNA as endogenous control in endometrial tissue

2005 ◽  
Vol 15 (2) ◽  
pp. 343-348
Author(s):  
M. Paul-Samojedny ◽  
A. Witek ◽  
A. Samojedny ◽  
A. Witkowska ◽  
T. Wilczok
Keyword(s):  
Reverse Transcriptase ◽  
Endometrial Hyperplasia ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Endometrial Adenocarcinoma ◽  
Endometrial Tissue ◽  
Endogenous Control ◽  
Telomerase Rna ◽  
Normal Endometrium ◽  
Human Telomerase

Telomerase is a reverse transcriptase that adds repetitive telomere sequences to the end of chromosomes, which is thought to be essential for cellular immortality and oncogenesis. The enzyme consists of three subunits: human telomerase reverse transcriptase (hTERT), human telomerase RNA (hTR), and telomerase protein 1 (TP1). The hTERT subunit determines the activity of telomerase as an enzyme and is detected in most human tumors and regenerative cells. But many studies have revealed that hTR and TP1 are expressed constitutively. This resuts suggest that the hTR and TP1 subunits may be potentially good markers of endogenous RNA control. Endometrial dating was determined from the pathomorphology of the endometrium and classified into normal proliferative endometrium, endometrial hyperplasia (simple, complex, and atypical), and endometrial adenocarcinoma. The analysis of the expression of the hTERT, TP1, and hTR telomerase subunits was performed by a quantitative polymerase chain reaction method, based on fluorescent TaqMan methodology (ABI Prism 7 700 Sequence Detection System) capable of measuring fluorescence in real time. The aim of the study was an analysis of the expression profiles of genes encoding hTR and TP1 telomerase subunits in normal endometrium, endometrial hyperplasia, and adenocarcinoma forthe estimation of the possibility of once application in endogenous RNA control of gene analysis in the endometrium. The nonparametric Mann–Whitney U test and analysis of variance Friedman test were used to evaluate the variation in telomerase subunit mRNA level between normal endometrium, and endometrial hyperplasia and adenocarcinoma. The results confirm the hTR subunit expression as a good marker of endogenous control in quantitative analysis of gene transcription in endometrial tissue. TP1 subunit transcriptions have not been detected constitutively in our study.

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