Impact of coherent light on interaction of fungi and bacteria cells cultivated in vitro

This article considers impact of coherent red quasi-monochromatic light on interaction of colonies of the Pseudomonas syringae bacteria and the Fusarium macroceras fungus in an in vitro culture. A helium-neon laser and a heat source with a system of light filters and aperture diaphragms were used for irradiation. Two light fluxes were obtained with energy parameters close in magnitude, but significantly different in spatio-temporal coherence. Light with a high statistical ordering stimulated growth of both colonies. Irradiation from the same spectral range and intensity, but with low spatial coherence, increased the functional activity of only small bacteria cells. As a result, there was a suppression of larger fungal cells development that were interacting with them. Therefore, it was the statistical (coherent) properties of light that affected the change in the equilibrium of microorganisms in an artificial biocenosis. This approach can be used in practice for increasing the activity of bacteria antagonists of pathogenic fungi and the non-chemical disease protection of plants.

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CHANGES IN FIBROBLAST PROLIFERATION AND METABOLISM FOLLOWING IN VITRO HELIUM-NEON LASER IRRADIATION

LASER THERAPY ◽

10.5978/islsm.91-or-04 ◽

1991 ◽

Vol 3 (1) ◽

pp. 25-33 ◽

Cited By ~ 18

Author(s):

J. Rigau ◽

M.A. Trelles ◽

R.G. Calderhead ◽

E. Mayayo

Keyword(s):

Laser Irradiation ◽

Fibroblast Proliferation ◽

Neon Laser ◽

Helium Neon Laser ◽

Helium Neon

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Physical and Mathematical Considerations About Laser Perimetry

Acta geneticae medicae et gemellologiae ◽

10.1017/s1120962300024069 ◽

1974 ◽

Vol 23 (S1) ◽

pp. 349-351 ◽

Cited By ~ 1

Author(s):

L. Favella ◽

L. Liuzzi ◽

F. Bartoli

Keyword(s):

White Light ◽

Laser Light ◽

Monochromatic Light ◽

Neon Laser ◽

Helium Neon Laser ◽

Helium Neon ◽

Mathematical Characteristics

The physical and mathematical characteristics of white light, monochromatic light, and laser light, are discussed with reference to the advantages offered by perimetry using a red helium-neon laser.

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Effect of helium-neon laser on the limbal zone cells of human eye

Journal of New Medical Technologies eJournal ◽

10.12737/10419 ◽

2015 ◽

Vol 9 (1) ◽

pp. 0-0

Author(s):

Сабурова ◽

I. Saburova ◽

Копаева ◽

V. Kopaeva ◽

Копаев ◽

...

Keyword(s):

Stem Cells ◽

Cell Culture ◽

Progenitor Cells ◽

Low Energy ◽

Limbal Stem Cells ◽

Neon Laser ◽

Helium Neon Laser ◽

Stem And Progenitor Cells ◽

Helium Neon

Currently, a helium-neon laser is widely used in the treatment of degenerative, inflammatory and vascular diseases of the eye. It was found that the interaction of this radiation with different tissues, as a result of complex photochemical processes, is manifested by anti-inflammatory, desensitizing, resolving effects. Also, there is a stimulating effect on the processes of reparation and trophism. However, these observations relate to the use of laser for external irradiation, when the light goes through the air and the sclera or through the cornea. Research of the effect of endo-laser direct exposure of the fiber in the cavity of the eye, where light acts directly on epithelial or limbal cells, wasn’t carry out due to objective reasons (difficulty of conducting such research directly to patients). There is currently no data on the effect of helium-neon laser on the limbal area of the eye-ball, enriched endogenous stem and progenitor cells. They help repair damaged tissues in the eye (anterior and posterior segment) at various injuries. Cell culture, grown in vitro, are free from the influences of body systems, attract researchers as a unique model to study the behavior of cells in normal but also in response to external or internal factors. Currently, scientists have learned how to obtain and difficult to cultivate a culture of cells, in-cluding tissues of the human eye. They are genetically homogeneous population of cells growing in constant conditions. The viability and morphology of cells and their ultra-structure and various molecular biological cha-racteristics can be studied in cell culture, and to find the optimal conditions of laser irradiation, does not damage the cells. In the present work the authors studied the effect of low-energy helium-neon laser irradiation (632 nm) on limbal stem cells. Conducted in vitro studies have shown that the use of low-energy helium-neon laser irrad-iation (632 nm) has a positive effect on the monolayer culture of limbal stem cells. Absence of changes in cell phenotype and high proliferative activity indicate a stimulating effect of the investigated radiation on stem and progenitor cells, leading to the result of the activation of recovery and reduction of pathological changes at the cellular and organ and tissue levels.

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