Development of rice variety with blast resistance genes Pi-b and Pi-z basing on DNA-technologies

The article presents stages of development and testing new rice variety Nautilus with blast resistance genes Pi-b and Pi-z. Basing on the use of DNA-marking methods, breeding material carrying the target genes was developed. The obtained material was studied in the links of breeding process in the field. Based on the results of field testing, 6 lines were identified, of which VNIIR 6016 turned out to be the best in terms of a complex of economically valuable traits. In 2016, this sample was transferred to the State variety testing under the name Nautilus. In 2019, a new rice variety Nautilus was included in the register of breeding achievements and approved for use in the Russian Federation in Krasnodar region. The study of variety Nautilus in 2019-2020 in an ecological test confirmed its high resistance to blast, in comparison with other released varieties. It took 10 - 12 years to develop the variety.

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Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production

Rice Science ◽

10.1016/j.rsci.2021.07.009 ◽

2021 ◽

Vol 28 (5) ◽

pp. 493-500

Author(s):

Vishalakshi Balija ◽

Umakanth Bangale ◽

Senguttuvel Ponnuvel ◽

Kalyani Makarand Barbadikar ◽

Srinivas Prasad Madamshetty ◽

...

Keyword(s):

Drought Tolerance ◽

Resistance Genes ◽

Upland Rice ◽

Blast Resistance ◽

Rice Variety ◽

Rice Production ◽

Blast Resistance Genes

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Identification of Blast-Resistance Genes in Korean Rice Variety, Tongil

Ikushugaku zasshi ◽

10.1270/jsbbs1951.30.73 ◽

1980 ◽

Vol 30 (1) ◽

pp. 73-82 ◽

Cited By ~ 3

Author(s):

Shigehisa KlYOSAWA ◽

CHO Ik

Keyword(s):

Resistance Genes ◽

Blast Resistance ◽

Rice Variety ◽

Blast Resistance Genes ◽

Korean Rice

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Rice Breeding in Russia Using Genetic Markers

Plants ◽

10.3390/plants9111580 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1580

Author(s):

Elena Dubina ◽

Pavel Kostylev ◽

Margarita Ruban ◽

Sergey Lesnyak ◽

Elena Krasnova ◽

...

Keyword(s):

Resistance Genes ◽

Target Genes ◽

Blast Resistance ◽

Soil Salinization ◽

Water Flooding ◽

Rice Varieties ◽

Asian Rice ◽

Southern Russia ◽

Tolerance Gene ◽

Blast Resistance Genes

The article concentrates on studying tolerance to soil salinization, water flooding, and blast in Russian and Asian rice varieties, as well as hybrids of the second and third generations from their crossing in order to obtain sustainable paddy crops based on domestic varieties using DNA markers. Samples IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, and NSIC Rc 106 were used as donors of the SalTol tolerance gene. Varieties with the Sub1A locus were used as donors of the flood resistance gene: Br-11, CR-1009, Inbara-3, TDK-1, and Khan Dan. The lines C101-A-51 (Pi-2), C101-Lac (Pi-1, Pi-33), IR-58 (Pi-ta), and Moroberekan (Pi-b) were used to transfer blast resistance genes. Hybridization of the stress-sensitive domestic varieties Novator, Flagman, Virazh, and Boyarin with donor lines of the genes of interest was carried out. As a result of the studies carried out using molecular marking based on PCR in combination with traditional breeding, early-maturing rice lines with genes for resistance to salinity (SalTol) and flooding (Sub1A), suitable for cultivation in southern Russia, were obtained. Introgression and pyramiding of the blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b into the genotypes of domestic rice varieties were carried out. DNA marker analysis revealed disease-resistant rice samples carrying 5 target genes in a homozygous state. The created rice varieties that carry the genes for blast resistance (Pentagen, Magnate, Pirouette, Argamac, Kapitan, and Lenaris) were submitted for state variety testing. The introduction of such varieties into production will allow us to avoid epiphytotic development of the disease, preserving the biological productivity of rice and obtaining environmentally friendly agricultural products.

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Integrating marker-assisted selection and doubled haploidy for rapid introgression of semi-dwarfing and blast resistance genes into a Basmati rice variety ‘Ranbir Basmati’

Euphytica ◽

10.1007/s10681-019-2473-7 ◽

2019 ◽

Vol 215 (9) ◽

Cited By ~ 1

Author(s):

Prabhudutt Samal ◽

T. D. Pote ◽

S. Gopala Krishnan ◽

Ashok K. Singh ◽

R. K. Salgotra ◽

...

Keyword(s):

Resistance Genes ◽

Marker Assisted Selection ◽

Blast Resistance ◽

Rice Variety ◽

Basmati Rice ◽

Doubled Haploidy ◽

Blast Resistance Genes

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Introgression of blast resistance genes into the elite rice variety MR263 through marker-assisted backcrossing

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.7222 ◽

2015 ◽

Vol 96 (4) ◽

pp. 1297-1305 ◽

Cited By ~ 7

Author(s):

Muhammad M Hasan ◽

Mohd Y Rafii ◽

Mohd Razi Ismail ◽

Maziah Mahmood ◽

Md Amirul Alam ◽

...

Keyword(s):

Resistance Genes ◽

Blast Resistance ◽

Rice Variety ◽

Marker Assisted Backcrossing ◽

Blast Resistance Genes

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Marker assisted rice breeding for resistance to biotic and abiotic stressors

BIO Web of Conferences ◽

10.1051/bioconf/20202100012 ◽

2020 ◽

Vol 21 ◽

pp. 00012

Author(s):

Elena Dubina ◽

Pavel Kostylev ◽

Sergey Garkusha ◽

Margarita Ruban ◽

Dmitry Pischenko

Keyword(s):

Resistance Genes ◽

Target Genes ◽

Blast Resistance ◽

Control Factor ◽

Rice Varieties ◽

Hybrid Plants ◽

Abiotic Stressors ◽

Light Mineral ◽

New Generation ◽

Blast Resistance Genes

Due to the fact that blast (causative agent – Pyricularia oryzae Cav.) is considered to be one of the harmful diseases of rice around the world, weeds compete with the crop for light, mineral nutrition and space, the accelerated development of resistant genotypes for these stressors is very relevant. The use of modern biotechnological approaches (molecular marking) is promising and especially in demand in breeding rice varieties of a new generation. This article presents the results on the introduction and pyramiding in the same genotype blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b, Pi-40 and the gene for tolerance to prolonged flooding Sub 1A, as a weed control factor, based on domestic rice varieties Flagman, Snezhinka, Novator, Boyarin, as well as large-grain lines, with a short growing season VNIIR5242, KP-25-14, KP-163 and VNIIR9678. As a result of the volumetric work using marker control of target genes in the genotypes of hybrid plants, 4 modern varietal samples and more than 400 backcross self-pollinated rice lines with introduced and pyramided blast resistance genes, as well as backcross self-pollinated lines with Pi and Sub1A genes, were obtained. These plants are adapted for cultivation in the south of Russia, have a duration of 115-117 days, a height of 87-100 cm, a mass of 1000 grains – 30 or more grams, a yield of 8.5 – 11 t/ha, which is significantly higher than that of the standard variety Flagman.

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