scholarly journals Microbiological evaluation of certain fruits and vegetables marketed in the city of Kenitra-Morocco

2021 ◽  
Vol 319 ◽  
pp. 01112
Author(s):  
Rahma Erahioui ◽  
Sultana Inekach ◽  
Hassna Jaber ◽  
Khadija Atfaoui ◽  
Noureddine Rhaim ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Microbiological Analysis ◽  
Microbial Quality ◽  
Total Absence ◽  
Nutritional Qualities ◽  
Raw Foods ◽  
The City

Fruits and vegetables are essential for human health due to their nutritional qualities, but the consumption of raw foods leads to food safety problems as they are recognized as sources of transmission of infectious diseases. The objective of this work is to assess the microbiological quality of some fruits and vegetables marketed in the Kenitra city. The samples were purchased at the fruit and vegetable markets during the period from April to July 2018.The detection of microorganisms in the samples requires several steps which are weighing, dilution, isolation, enumeration and 'identification. The results of the microbiological analysis of fruits and vegetables studied show significant concentrations of the total aerobic mesophilic flora varies between 2.25 and 8.48 log10 CFU / ml, Concerning total coliforms the contamination rates vary between 1.92 and 8.42 log10UFC / ml, as well as the presence of fecal coliforms between 1.13 and 8.05 log10UFC / ml, in the same samples analyzed, strains such as: Escherichia coli, staphylococcus aureus and a total absence of salmonella and shegilla. In order to improve the safety and hygienic quality of fruits and vegetables, the application of good hygienic practices as well as continuous microbial quality control is necessary to protect the health of consumers.

Minimally Processed Vegetable Salads: Microbial Quality Evaluation

2007 ◽  
Vol 70 (5) ◽  
pp. 1277-1280 ◽  
Author(s):  
HANS FRÖDER ◽  
CECÍLIA GERALDES MARTINS ◽  
KATIA LEANI OLIVEIRA de SOUZA ◽  
MARIZA LANDGRAF ◽  
BERNADETTE D. G. M. FRANCO ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Positive Sample ◽  
Fecal Coliforms ◽  
Microbial Quality ◽  
Biochemical Tests ◽  
Minimally Processed ◽  
Increasing Demand ◽  
Minimally Processed Vegetables

The increasing demand for fresh fruits and vegetables and for convenience foods is causing an expansion of the market share for minimally processed vegetables. Among the more common pathogenic microorganisms that can be transmitted to humans by these products are Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella. The aim of this study was to evaluate the microbial quality of a selection of minimally processed vegetables. A total of 181 samples of minimally processed leafy salads were collected from retailers in the city of Sao Paulo, Brazil. Counts of total coliforms, fecal coliforms, Enterobacteriaceae, psychrotrophic microorganisms, and Salmonella were conducted for 133 samples. L. monocytogenes was assessed in 181 samples using the BAX System and by plating the enrichment broth onto Palcam and Oxford agars. Suspected Listeria colonies were submitted to classical biochemical tests. Populations of psychrotrophic microorganisms >106 CFU/g were found in 51% of the 133 samples, and Enterobacteriaceae populations between 105 and 106 CFU/g were found in 42% of the samples. Fecal coliform concentrations higher than 102 CFU/g (Brazilian standard) were found in 97 (73%) of the samples, and Salmonella was detected in 4 (3%) of the samples. Two of the Salmonella-positive samples had <102 CFU/g concentrations of fecal coliforms. L. monocytogenes was detected in only 1 (0.6%) of the 181 samples examined. This positive sample was simultaneously detected by both methods. The other Listeria species identified by plating were L. welshimeri (one sample of curly lettuce) and L. innocua (2 samples of watercress). The results indicate that minimally processed vegetables had poor microbiological quality, and these products could be a vehicle for pathogens such as Salmonella and L. monocytogenes.

scholarly journals Physico-chemical and microbiological characteristics of water for domestic use in Cotonou in the republic of Benin

2021 ◽  
Vol 4 (1) ◽  
pp. 188-197
Author(s):  
Honoré Ubald M Adande ◽  
Pauline Houdagba, Rodrigue C Landeou ◽  
Expédit W Vissin
Keyword(s):  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Household Waste ◽  
Microbiological Analysis ◽  
Domestic Water ◽  
Household Level ◽  
Physico Chemical ◽  
Republic Of Benin ◽  
The City

Water is the source of life. It is essential for the survival of all living things. However, its alteration becomes harmful to humans. In Cotonou, the main city of Benin, the quality of drinking water is worrying because of many factors such as environmental pollution and poor hygiene around water sources. In fact, poorly ecological management methods of household waste pose risks of contamination of soil and water resources. This study aims to assess the quality of domestic water for households in the city of Cotonou. The methodology adopted is summed up in a physico-chemical and microbiological analysis of thirty water samples taken from households in the 13 districts of the city of Cotonou. The results obtained at the physicochemical level show that, apart from the waters of SONEB at the source, whose turbidity (5.6 NTU) complies with the standard which is 5 NTU, that of other sources is much greater than Standard. This indicates the presence of suspended solids in the water that may harbor microorganisms harmful to humans. With regard to microbiological quality, all the water sampled at household level contains E.Coli, the number of which is much higher than the Benin standard which sets at 0/1 ml of water. In addition, the water sampled from the wells contains high numbers of fecal coliforms. As for SONEB water, the presence of coliforms was determined but with lower numbers than well water (50 / ml at household level and 2 / ml at source level.

scholarly journals Clarification of the Algerian grape juice and their effects on the juice quality

2020 ◽  
Vol 34 (1) ◽  
pp. 1-11
Author(s):  
Soraya Mazrou ◽  
Mohammed Messaoudi ◽  
Samir Begaa ◽  
Christophe Innocent ◽  
Djamaleddine Akretche
Keyword(s):  
Condensed Tannins ◽  
Microbiological Quality ◽  
Grape Juice ◽  
Juice Quality ◽  
Microbial Quality ◽  
Total Polyphenol ◽  
Accelerated Stability ◽  
Nutritional Qualities ◽  
And Storage

The aim of this study was to find a good clarification method to eliminate the substances in Algerian grape juice and study the effects of the clarification agents on juice quality. The clarified grape juice was subjected to different treatments, namely bentonite, gelatin, combination of gelatin and bentonite, then stored for 4 weeks. The effects of fining treatment by determining the critical micillary concentration of each agent used accelerated stability test on turbidity, tannin contents (ethanol index, condensed tannins, total polyphenol and anthocyanins) and microbiological quality of clarified grape juice were evaluated during storage. Fining treatment and storage had a significant (p < 0.05) effect on turbidity, tannins, total polyphenol, condensed tannins and anthocyanins. However, a better percentage elimination was noted for freshly squeezed grape juice with a combination of gelatin and bentonite corresponding to a turbidity of 6.5 NTU. The clarifiers separately gave lower removal rates than average, up to 83% of tannins present in the freshly squeezed juice has been eliminated using the bentonite. The results obtained are very satisfactory since we were able to obtain a clear juice of good microbial quality while ensuring the preservation of the organoleptic and nutritional qualities during the treatment and the storage of the juice.   Bull. Chem. Soc. Ethiop. 2020, 34(1), 1-11. DOI: https://dx.doi.org/10.4314/bcse.v34i1.1

scholarly journals AVALIAÇÃO MICROBIOLÓGICA DE HORTALIÇAS E FRUTAS MINIMAMENTE PROCESSADAS COMERCIALIZADAS EM FORTALEZA (CE)

2005 ◽  
Vol 23 (1) ◽  
Author(s):  
LAURA MARIA BRUNO ◽  
ANA AMÉLIA MARTINS DE QUEIROZ ◽  
ANA PAULA COLARES DE ANDRADE ◽  
NATÁLIA MOURA DE VASCONCELOS ◽  
MARIA DE FÁTIMA BORGES
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Minimally Processed ◽  
Total Coliforms ◽  
Vegetable Samples ◽  
Fruit Samples ◽  
Vegetables And Fruits ◽  
Coagulase Positive Staphylococcus

Avaliou-se a qualidade microbiológica de 15 amostras de hortaliças/tubérculos e de 15 amostras de frutas minimamente processadas, comercializadas em Fortaleza (CE). Foram realizadas análises de coliformes fecais e totais, pesquisa de Salmonella sp., contagem total de bolores e leveduras, contagem de Staphylococcus coagulase positiva e contagem total de psicrotróficos. Detectou-se a presença de Salmonella em 66,6% das amostras de hortaliças/tubérculos e em 26% das de frutas. Foi verificado que 13,3% das amostras de hortaliças/ tubérculos apresentaram contagem de coliformes fecais acima do limite estabelecido pela legislação brasileira. Contagens elevadas de coliformes totais, de bolores e leveduras e de psicrotróficos também foram encontradas em ambos os produtos, indicando condições inadequadas de higiene durante o processamento, comprometendo seu armazenamento e sua qualidade microbiológica. MICROBIOLOGICAL EVALUATION OF VEGETABLES AND FRUITS MINIMALLY PROCESSED MARKETED IN FORTALEZA (CE) Abstract The microbiological quality of 15 vegetables and 15 fruits minimally processed samples marketed in Fortaleza - CE (Brazil) was evaluated. The following analyses were carried out: total and fecal coliforms, Salmonella sp., total yeasts and moulds count, coagulase positive Staphylococcus count and total psychrotrophics count. Salmonella was detected in 66.6% of vegetable samples and 26% of fruit samples. Fecal coliforms count higher than legislation limit, it was verified in 13.3% of vegetable samples. High counts of total coliforms, yeasts and moulds and psychrotrophics were also found on both products, showing inadequate hygiene conditions during manufacturing, affecting storage and microbiological quality of minimally processed fruits and vegetables.

scholarly journals Avaliação microbiológica de queijo de coalho comercializado na feira livre de Sousa - Paraíba

2017 ◽  
Vol 1 (37) ◽  
pp. 85
Author(s):  
Diego Ernani Leite Bezerra ◽  
Carlos Roberto Marinho da Silva Filho ◽  
Damião Júnior Gomes ◽  
Ednaldo Barbosa Pereira Junior
Keyword(s):  
Microbiological Quality ◽  
Microbiological Analysis ◽  
Federal Institute ◽  
Good Taste ◽  
Good Manufacturing Practices ◽  
Almost All ◽  
Education Science ◽  
The City ◽  
Coagulase Positive Staphylococci

<p class="Default">For many years the curd cheese has stood out on the table of Brazilian consumers, especially in the Northeast due to its high nutritional value and its good taste and because of that it has been used in numerous dishes of the Northeastern cuisine. The aim of this study was to evaluate the microbiological quality of the curd cheese sold at a market garden in the city of Sousa-PB. Twenty curd cheese samples were collected every week during the month of October 2015, in five sales areas at a market garden in the city. Samples were analyzed at the Microbiological Analysis of Foods Laboratory of the Agroindustry sector at the Federal Institute of Education, Science and Technology of Paraíba, Campus Sousa, having as reference the Resolution of the Collegiate Board (RDC) n ° 12 of the National Sanitary Surveillance Agency. According to the results, the values obtained for total coliforms were considered high in almost all the samples. Regarding coliform, seven out of twenty samples (35%) were above the threshold count allowed by Brazilian law. As for the pathogenic to 65% of the samples presented coagulase positive Staphylococci counts and in 40% the growth of Salmonella sp,it was confirmed. Based on the results obtained,it can be said that these products are being manufactured without the adoption of Good Manufacturing Practices, which a condition that compromises the quality of the product and puts the health of consumers at risk</p>

Survey of the Microbiological Quality of Whole, Black Pepper and Turmeric Powder Sold in Retail Shops in Bombay

1987 ◽  
Vol 50 (5) ◽  
pp. 401-403 ◽  
Author(s):  
H. GEETA ◽  
P. R. KULKARNI
Keyword(s):  
Microbiological Quality ◽  
Black Pepper ◽  
Poor Quality ◽  
International Standards ◽  
Microbiological Analysis ◽  
Fungal Flora ◽  
Plate Counts ◽  
The City

Microbiological analysis of loosely packed, whole, black pepper and turmeric powder obtained from retail shops in the city of Bombay revealed that the samples of both spices were highly contaminated. Aerobic plate counts of black pepper ranged from 12.1 × 107 to 81.9 × 108 c.f.u. per gram and turmeric powder from 4.1 × 107 to 73.6 × 108 c.f.u. per g. In both spices, mesophilic sporeformers like Bacillus occurred. Coliforms ranged in counts from 102-103 per g. Fungal counts ranged from 0.6 × 104 to 16 × 105 per g for black pepper and from 0.5 × 103 to 11.1 × 105 per g for turmeric powder. Fungal flora included mainly Aspergillus spp. with the occurrence of Mucor in some of the samples. No other organisms were observed in the dilutions plated. The extent of contamination was slightly greater in pepper than in turmeric, although both spices were of a poor quality when compared with international standards.

scholarly journals Physical-chemical and microbiological analysis of water of irrigation and microbiological analysis of lettuce (Lactuca sativa)

2019 ◽  
Vol 41 ◽  
pp. e55
Author(s):  
Camila Corrêa Bierhas ◽  
Aline Belem Machado ◽  
Simone Ulrich Picoli ◽  
Daniela Montanari Migliavacca Osorio ◽  
Daiane Bolzan Berlese
Keyword(s):  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Microbiological Analysis ◽  
Microbiological Contamination ◽  
Physical Chemical ◽  
Quality Of Water ◽  
Three Samples ◽  
Almost All

The contamination of vegetables by pathogenic microorganisms is directly related to the water quality used in their irrigation. Lettuce is the main vegetable consumed in Brazil and because it does not undergo any processing before its consumption, it is directly affected by the quality of the water used for irrigation. This study analyzed the physical-chemical and microbiological quality of water used in lettuce irrigation and possible microbiological contamination of lettuce. In relation to microbiological analyzes, high values were found for heterotrophic bacteria and total coliforms in weirs and vegetables. For fecal coliforms, in almost all water samples, the value found was above that established by the legislation. In lettuce, this occurred in three samples. No strong correlation was found between water and lettuce contamination. In relation to the physicochemical parameters analyzed, only the value of the turbidity in one of the weirs was above the threshold established by the legislation.

scholarly journals Microbiological Quality of Egusi Pudding, A Traditional Cake of Cucurbitaceae Sold in the City of Yaoundé, Cameroon

2022 ◽  
Vol 2022 ◽  
pp. 1-12
Author(s):  
Ronice Zokou ◽  
Hippolyte Tene Mouafo ◽  
Julie Mathilde Klang ◽  
Noutsa Boris Simo ◽  
Raymond Simplice Mouokeu ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Threshold Value ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Protein Sources ◽  
Staphylococcus Spp ◽  
And Control ◽  
The City

Egusi pudding is one of the most popular traditional dishes of the Cameroonian population. Besides its nutritional values, it is also endowed with a sociocultural character. Nowadays, consumer demand for Egusi pudding has increased and the dish is sold as street food in several cities of Cameroon and mostly under uncontrolled hygienic conditions. The objective of this study was to assess the microbiological quality of Egusi pudding sold in the city of Yaoundé taking into consideration the protein sources and the sampling sites. Five types of Egusi pudding samples based on protein sources were randomly collected from 25 sellers distributed in 7 districts in the city of Yaoundé and their microbiological quality were assessed. The results showed that the total aerobic count of the different samples (2.97 ± 0.03 to 4.43 ± 0.05 Log CFU/g) was under the threshold value (5.47 Log CFU/g) recommended for food intended for human consumption. Loads of fecal coliforms (1.47 ± 0.00 to 5.47 ± 0.00 Log CFU/g) in 40% of samples, Escherichia coli (2.39 ± 0.12 to 5.43 ± 0.05 Log CFU/g) in 60% of samples, fecal streptococci (2.90 ± 0.15 to 4.74 ± 0.05 Log CFU/g) in 88% of samples, Pseudomonas spp. (3.39 ± 0.15 to 5.43 ± 0.06 Log CFU/g) in 100% of samples, and the presence of Salmonella spp. in 56% of samples revealed a poor level of hygiene of the vendors. Pathogens associated with unsafe food handling such as Staphylococcus spp. were found in 100% of samples at loads (3.84 ± 0.18 to 5.43 ± 0.05 Log cfu/g) higher than the norms of the European Commission. Potential toxigenic pathogens such as Clostridium perfringens, Yersinia enterocolitica, Bacillus cereus, and moulds were also found, respectively, in 100, 96, 96, and 100% of samples. Overall, the most contaminated samples were those made with sardine as protein source, 92.85% (n = 23/25), followed with beef (88.57%), mackerel (84.28%), cod (82.85%), and control (77.1%). The results of this study suggest that important measures should be taken by the Public Health Service in order to sensitize the producers and vendors of Egusi pudding on the respect of good hygiene, manufacturing practices, and the continuous monitor of the quality of traditional products sold in markets.

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from &lt;3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

VERMICOMPOSTING TECHNOLOGY FOR STABILIZING THE SEWAGE SLUDGE FROM RURAL WASTE WATER TREATMENT PLANTS

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
V. Lina Cardoso ◽  
C. Esperanza Ramírez ◽  
E. Violeta Escalante
Keyword(s):  
Rural Communities ◽  
Sewage Sludge ◽  
Low Cost ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Nitrogen And Phosphorus ◽  
Sludge Management

There are problems associated with sludge management in small treatment plants (&lt;10 L/s) located in rural communities, due to costly conventional technology for sludge stabilization. Many of these plants have only sludge drying beds. Mexican Institute of Water Technology has proposed developing suitable low-cost technologies, one of which is vermicomposting a biodegradation system using earthworms of the species Eisenia foetida (earthworm) which stabilize sludge and reduce its pathogenicity. The objective of this work is to present two case studies where vermicomposting technology has been applied in Mexico. The first study corresponds to a plant where 4.8 m3/month of sludge are produced; for these wastes, a vermicomposting system was built and installed. The second study is a treatment plant where 9 m3/month of sludge are produced; experimental tests were conducted with sludge and water hyacinth and a vermicomposting system was designed. The vermicomposts were analyzed using parameters defined by Mexican standards. In regards to stabilization, TVS was reduced by 38% and the microbiological quality of the vermicompost was Class A and B, with a reduction in fecal coliforms and Helminth eggs according to NOM-004-SEMARNAT-2002. A CRETI (Corrosivity, Reactivity, Explosivity, Toxicity and Ignitability) analysis (NOM-052-SEMARNAT-2005) was used to show that the process reduced the concentration of releasable sulfides. The agronomic quality of the vermicompost exhibited a high content of organic matter comparable to many organic manures and high content of nutrients such as nitrogen and phosphorus. It is concluded that it is possible to improve the conditions of sewage sludge management in small plants of rural communities with a minimum investment (less than $10,000.00 USD) and with a requirement of a minimum area of 60 to 70 m2 for a production of less than 9 m3/month of dehydrated sludge (80% humidity).

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