TB PCR in the early diagnosis of tuberculous meningitis: evaluation of the Roche semi-automated COBAS Amplicor MTB test with reference to the manual Amplicor MTB PCR test

2000 ◽  
Vol 80 (4-5) ◽  
pp. 191-196 ◽  
Author(s):  
A. Bonington ◽  
J.I.G. Strang ◽  
P.E. Klapper ◽  
S.V. Hood ◽  
A. Parish ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Tuberculous Meningitis ◽  
Tb Pcr ◽  
Pcr Test

Early diagnosis of tuberculous meningitis by polymerase chain reaction

Neurology ◽  
1995 ◽  
Vol 45 (12) ◽  
pp. 2228-2232 ◽  
Author(s):  
L. F.F. Kox ◽  
S. I. Kuijper ◽  
A. H.J. Kolk
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Diagnosis ◽  
Tuberculous Meningitis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals TRC4 Gene Based PCR Assay in Diagnosis of Tuberculous Meningitis

2017 ◽  
Vol 8 (2) ◽  
pp. 19-22
Author(s):  
Abu Naser Ibne Sattar ◽  
Sanjida Khondakar Setu ◽  
Ahmed Abu Saleh ◽  
Sharmeen Ahmed
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Manifestation ◽  
Tuberculous Meningitis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Early Stages ◽  
Lowenstein Jensen ◽  
Polymerase Chain ◽  
Tb Pcr ◽  
Is6110 Element

The prevalence of Tuberculous meningitis remains largely underestimated due to nonspecific clinical manifestation at early stages. A total of 20 CSF samples were studied from clinically suspected cases of tuberculous meningitis. All the samples were processed for ZN staining, TB culture on LJ media and TB-PCR using IS6110 and TRC4 primers by standard protocols. Of the total 20 CSF samples, 12 cases were diagnosed as TB meningitis. Most of the tuberculous meningitis cases were found positive by PCR using TRC4 primers (83.33%) followed by IS6110 primer (66.67%) and culture on L-J media(8.33%). None were found positive by ZN staining. TB-PCR usingTRC4 primer showed higher positivity than using IS6110 in detecting tuberculous meningitis, since some strains of MTB may lack the IS6110 element in their genome. PCR assay using TRC4 primer is superior in diagnosing tuberculous meningitis. This study was aimed to evaluate the diagnostic potentials of CSF polymerase chain reaction (PCR) by using TRC4 and IS6110 primers. Further the results were also compared with culture on Lowenstein-Jensen (LJ) media and AFB smear by Zeihl-Nelson (ZN) staining.Bangladesh J Med Microbiol 2014; 08 (02): 19-22

scholarly journals Early Diagnosis of Tuberculous Meningitis

BMJ ◽  
1947 ◽  
Vol 2 (4525) ◽  
pp. 507-507
Author(s):  
M. C. Wilkinson
Keyword(s):  
Early Diagnosis ◽  
Tuberculous Meningitis

Ventricular biopsy for early diagnosis in tuberculous meningitis

2013 ◽  
Vol 333 ◽  
pp. e714-e715
Author(s):  
S. Vijayarangam Shanmugam ◽  
B. Karupannan
Keyword(s):  
Early Diagnosis ◽  
Tuberculous Meningitis

Study on Early Diagnosis of Invasive Aspergillosis in Patients with Hematological Diseases.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4982-4982
Author(s):  
Ai-ning Sun ◽  
Jun Wang ◽  
De Pei Wu ◽  
Su-ning Chen
Keyword(s):  
Early Diagnosis ◽  
Invasive Aspergillosis ◽  
Antifungal Therapy ◽  
Predictive Value ◽  
Elisa Test ◽  
Hematological Disease ◽  
Sensitivity Specificity ◽  
Positive Results ◽  
Test Result ◽  
Pcr Test

Abstract Objective To evaluate the value of galactomannan(GM) detection and nest PCR for early diagnosis of invasive aspergillosis (IA) in patients with hematological disease. Methods GM ELISA and nest PCR was performed to serially screen for circulating galactomannan(GM) and DNA of Aspergillus ssp. Respectively, 96 patients were grouped according to case definitions of the EORTC, including 5 proven IA and 28 probable IA cases. Samples of the patients included serum twice weekly(326) and bronchoalveolar lavage fluid(BALF 12). Sensitivity, specificity and predictive values were calculated respectively and compared. Results According to the receiver operator characteristic curve(ROC), using a reduced cutoff of 0.5 O.D.I of two-positive GM test result, we can achieved the most optimal results. The sensitivity, specificity, positive predictive value (PPV)and negative predictive value (NPV) of the ELISA test were 85.7%,91.6%,85.7%,91.6%respectively. While for the BALF samples, a increased cutoff 1.0 maybe improve the value of this diagnosis, with sensitivity 50% and specificity 100%.Quantitive results of the serum-based GM antigen can represent the status of IA and were correlated with the prognosis Of IA.Galactomannanemia preceded the development of characteristic findings on CT about 6 days. The nest PCR assay amplifies specifically a region of the 18S rRNA gene that is highly conserved in Aspergillus species and allows detection of down to 5.0 fg/ml of Aspergillus DNA. When two-positive results were used to define an episode as ‘PCR positive’, the sensitivity, specificity, PPV and NPV of the PCR test were 92.9%, 100%,100%,96.0% respectively. For the BALF samples, the sensitivity and specificity decreased to 75%and 60%. The frequency of the consecutively positive results of a patient was correlated with prognosis Of IA. The antifungal therapy usually causes a intermittent positive PCR results. Positve PCR results preceded the development of characteristic findings on CT about 9 days. Conclusion The cutoff 0.5 of two-positive serum-based GM ELISA test result is the most optimal cutoff value. GM ELISA test is a rapid,reliable method for early diagnosis and treatment of IA, with good sensitivity(85.7%)and specificity(91.6%). GM ELISA test is a good direction for preemptive antifungal therapy in patients with hematological disease at risk. Using two-positive results to define an episode as ‘PCR positive’, great sensitivity(92.9%)and specificity(100%) could be achieved. The serum-based PCR test is useful for screening for Aspergillus spp. in patients with hematological disease at risk but without antifungal treatment.

scholarly journals EVALUATION OF DIAGNOSTIC PERFORMANCE OF GENEXPERT MTB IN CEREBROSPINAL FLUID FOR EARLY DIAGNOSIS OF TUBERCULOUS MENINGITIS

2019 ◽  
Vol 6 (33) ◽  
pp. 2253-2256
Author(s):  
Namita Mohapatra ◽  
Gurukurshna Mohapatra ◽  
Sankarsan Das ◽  
Choudhry Bijay Kumar Mohanty ◽  
Jigyansa Mohapatra
Keyword(s):  
Cerebrospinal Fluid ◽  
Early Diagnosis ◽  
Tuberculous Meningitis ◽  
Diagnostic Performance

scholarly journals Role of Magnetic Resonance Imaging and Spinal Tap in Early Diagnosis of Tuberculous Meningitis

2019 ◽  
Vol 8 (3) ◽  
pp. 127-130
Author(s):  
Salma Haji
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Early Diagnosis ◽  
Tuberculous Meningitis ◽  
Stage I ◽  
Resonance Imaging ◽  
Mri Brain ◽  
Csf Analysis ◽  
Magnetic Resonance Imaging Mri

Background: Tuberculous meningitis (TBM) is difficult to diagnose in early stages due to nonspecific symptoms. There should be high index of suspicion to diagnose TBM at an early stage. The objective of the study was to find out the role of magnetic resonance imaging (MRI) and spinal tap in early diagnosis of tuberculous meningitis. Material and Methods: A cross sectional study was conducted from July 2015 till July 2018 at Neuromedicine ward, Jinnah Postgraduate Medical Centre (JPMC), Karachi. All patients above 12 year of age, both male and female with nonspecific symptoms like headache, malaise and drowsiness or suspicion of TBM (stage I, II, and III according to British Medical Research Council TBM staging criteria) were included in the study. Patients diagnosed with other CNS disease like encephalitis, malaria and acute bacterial meningitis were excluded. Magnetic Resonance Imaging (MRI) of the brain and early spinal tap for cerebrospinal fluid (CSF) analysis were used to diagnose TBM and findings were noted. Results of MRI and CSF analysis were analyzed by SPSS version 24. Results: A total of 110 patients of TBM, with 60 (54.5%) male and 50 (45.5%) female patients were included in the study. Most of the patients belonged to a younger age group of 12-40 years (81.8%), while 18.2% were above 40 years of age. About 90% patients were diagnosed in stage I TBM and 10% in stage II and III. MRI brain findings included meningeal enhancement (60%), hydrocephalus (41.81%) cerebral edema (82.73%), tuberculoma (19%) and infarct (14.5%), respectively. CSF analysis showed low protein in 80%, low glucose in 91.8% and lymphocytic pleocytosis in 97.2%, respectively. Conclusion: Both MRI brain and spinal tap with CSF analysis played a role in the early diagnosis of TBM, which is important to prevent the lethal complications associated with late diagnosis of this disease.

scholarly journals Efficacy of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis

2019 ◽  
Author(s):  
Se Chang Oh ◽  
Soo Min Park ◽  
Jian Hur ◽  
Eun Young Choi ◽  
Hyun Jung Jin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Diagnosis ◽  
Multiplex Pcr ◽  
Bordetella Pertussis ◽  
Turnaround Time ◽  
University Hospital ◽  
Diagnosis And Treatment ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pcr Test

Abstract Background Pertussis, a highly infectious respiratory disease caused by Bordetella pertussis, causes airway inflammation and severe, persistent (lasting 2 weeks or more) characteristic whooping cough. In severe cases, complications such as atelectasis and bronchopneumonia may occur. Recently, the prevalence of pertussis has increased in South Korea due to reduced effect of the DTaP vaccination in infants as their age increased. Although culture is the gold standard test for diagnosis, polymerase chain reaction (PCR) method is most commonly used for diagnosis of pertussis due to the low sensitivity and long turnaround time of the culture method. Recently, a rapid multiplex PCR test has been introduced for comprehensive detection of respiratory pathogens (17 viruses and 3 bacteria), including Bordetella pertussis, with a turnaround time of 1 hour. This study aimed to investigate the efficacy of multiplex PCR for early diagnosis and treatment of pertussis. Methods We performed a retrospective study on patients with pertussis diagnosed from May 2017 to June 2019 at Yeungnam University Hospital. Nasopharyngeal swab specimens were tested using multiplex PCR. Medical records collected included data on age, sex, symptoms at the time of diagnosis, admission, hospitalization, isolation, vaccination history, past medical history, and accompanying diseases. Results A total of 27 patients were diagnosed with pertussis, nine (33.3%) of whom were men, with a median age of 48.9 years (3.3–82.2). Eleven (40.7%) had fever, 12 (44.4%) had dyspnea, 3 (11.1%) had paroxysmal cough, and 9 (33.3%) had inspiratory whooping. Seventeen (62.9%) and 24 (88.8%) patients had coughing for <8 days and ≤14 days, respectively. Median time from first symptom to diagnosis was 9.0 (1–31) days. Twenty-four patients (81.5%) were diagnosed within 2 weeks. All but one patient was prescribed macrolide antibiotics; all patients were isolated, with 22 (81.5%) requiring hospitalization. Three patients (11.1%) received ICU care for ventilation. All patients survived. Conclusion A rapid multiplex PCR test can ensure early diagnosis, isolation, and treatment of pertussis. Testing of patients with respiratory symptom with multiplex PCR can led to early diagnosis of pertussis, proper treatment, and may help in outbreak control.

scholarly journals Sensitive enzyme immunoassay for early diagnosis of tuberculous meningitis.

1984 ◽  
Vol 20 (3) ◽  
pp. 533-535 ◽  
Author(s):  
R Hernández ◽  
O Muñoz ◽  
H Guiscafre
Keyword(s):  
Early Diagnosis ◽  
Enzyme Immunoassay ◽  
Tuberculous Meningitis
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