Combined Effects of Single Nucleotide Polymorphisms (SNPs) within
                    C-reactive Protein (CRP) and Environmental Parameters on Risk and Prognosis for
                    Diabetic Foot Osteomyelitis Patients

Abstract Purpose This study was aimed to discover the combined effects of single nucleotide polymorphisms (SNPs) within the C-reactive protein (CRP) gene and potential environmental factors on the risk and prognosis for diabetic foot osteomyelitis (DFO). Methods A total of 1734 diabetes mellitus patients, 681 with DFO and 1053 without DFO, were successfully recruited, as well as 1261 healthy control individuals. Participants data were recorded regarding age, gender, smoking and drinking history, body mass index (BMI), hypertension, cacosmia, and ulceration. A total of 11 SNPs within the CRP gene were designated for exploration, by logistic regression analyses, of how they might interact with environmental factors to affect susceptibility to DFO. Results Frequencies of smoking and drinking, and incidence of hypertension, cacosmia, or ulceration displayed marked differences (all P<0.05) between DFO and non-DFO patients. Furthermore, allele G of rs11265260 (A>G), allele G of rs1800947 (C>G), and allele T of rs3093059 (T>C) and rs1130864 (C>T) exhibited a trend to increase risk of DFO (all P<0.05). Allele G of rs11265260 (A>G), allele G of rs1800947 (C>G) and rs3093068 (G>C), and allele T of rs1130864 (C>T) were significant predictors of poor prognosis among DFO patients (P<0.05). In addition, genotypes of rs11265260 (i.e., GG and AG), rs1800947 (i.e., CG and GG), rs3093059 (i.e., TT) and rs113084 (i.e., CT and TT) amplified the influence of smoking, alcohol consumption, cacosmia, and ulceration on progression from non-DFO to DFO (all γ>1). Conclusion Genetic mutations within CRP functioned interactively with external factors to affect DFO risk.

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Single-nucleotide polymorphisms in the C-reactive protein (CRP) gene promoter that affect transcription factor binding, alter transcriptional activity, and associate with differences in baseline serum CRP level

Journal of Molecular Medicine ◽

10.1007/s00109-005-0658-0 ◽

2005 ◽

Vol 83 (6) ◽

pp. 440-447 ◽

Cited By ~ 114

Author(s):

A. J. Szalai ◽

J. Wu ◽

E. M. Lange ◽

M. A. McCrory ◽

C. D. Langefeld ◽

...

Keyword(s):

Transcription Factor ◽

Single Nucleotide Polymorphisms ◽

Transcriptional Activity ◽

Gene Promoter ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Baseline Serum ◽

Reactive Protein ◽

Serum Crp

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Influence of Single-Nucleotide Polymorphisms on C-Reactive Protein Levels in Chronic Kidney Disease Before and After Kidney Transplantation

Transplantation Proceedings ◽

10.1016/j.transproceed.2008.04.021 ◽

2008 ◽

Vol 40 (10) ◽

pp. 3349-3353

Author(s):

A.C. Cordeiro ◽

I. de Castro ◽

R. Pecoits-Filho ◽

R. Ramasawmy ◽

H. Rodrigues ◽

...

Keyword(s):

Chronic Kidney Disease ◽

Kidney Transplantation ◽

Kidney Disease ◽

Single Nucleotide Polymorphisms ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Protein Levels ◽

Reactive Protein ◽

Before And After

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Exploring the Effects of C-Reactive Protein (CRP) and Interleukin-1 β Single Nucleotide Polymorphisms on CRP Concentration in Patients With Established Coronary Artery Disease

International Heart Journal ◽

10.1536/ihj.48.463 ◽

2007 ◽

Vol 48 (4) ◽

pp. 463-475 ◽

Cited By ~ 6

Author(s):

Jou-Wei Lin ◽

Dao-Fu Dai ◽

Yi-Hua Chou ◽

Li-Ying Huang ◽

Juey-Jen Hwang

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Single Nucleotide Polymorphisms ◽

Interleukin 1 ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Established Coronary Artery Disease ◽

Reactive Protein ◽

Artery Disease

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Relationship between polymorphisms in the CRP, LEP and LEPR genes and high sensitivity C-reactive protein levels in Spanish children

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2017-0134 ◽

2017 ◽

Vol 55 (11) ◽

Cited By ~ 3

Author(s):

Pilar Navarro ◽

Olaya de Dios ◽

Teresa Gavela-Pérez ◽

Leandro Soriano-Guillen ◽

Carmen Garcés

Keyword(s):

Single Nucleotide Polymorphisms ◽

High Sensitivity ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Elisa Kit ◽

Protein Levels ◽

Reactive Protein ◽

Hs Crp

AbstractBackground:We investigated the association of single nucleotide polymorphisms (SNPs) in the C-reactive protein (Methods:We measured hs-CRP levels in 646 6–8-year-old and 707 12–16-year-old children using a high-sensitivity C-Reactive Protein ELISA kit. Four SNPs in theResults:The four CRP SNPs studied were significantly (p<0.05) associated with hs-CRP levels in both cohorts. Furthermore, two common CRP haplotypes (constructed using the SNPs in order: rs1205, rs1130864, rs1800947, rs2794521) ACGA and GCGG were associated with significantly lower CRP levels (p<0.05) at both ages. The LEPR SNPs rs1137100 (K109R) and rs1137101 (Q223R), and LEP SNP rs7799039 (G2548A) were also associated to hs-CRP levels (p<0.05) in both cohorts.Conclusions:hs-CRP levels in healthy Spanish children, besides being associated to common polymorphisms in the

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AB0001 Genotype and Haplotype Effects of 7 Single-Nucleotide Polymorphisms in the CRP Gene on Levels of C-Reactive Protein and DAS28 in Two Cohorts of Treatment NaÏVe, Recent Onset Rheumatoid Arthritis Patients

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2014-eular.1357 ◽

2014 ◽

Vol 73 (Suppl 2) ◽

pp. 804.1-804

Author(s):

C.G. Ammitzbøll ◽

R. Steffensen ◽

M. Bøgsted ◽

K. Hørslev-Petersen ◽

M.L. Hetland ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Recent Onset ◽

Reactive Protein ◽

Treatment Naïve

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Identifying single nucleotide polymorphisms (SNPs) and detecting c-reactive protein (CRP) levels associated with posterior circulation ischemia and cerebral infarction

10.21203/rs.3.rs-358781/v1 ◽

2021 ◽

Author(s):

Wanhua Chen ◽

Teng Lin ◽

Lei Xiao ◽

Wenjie Cai ◽

Zhiwei Zeng ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Cerebral Infarction ◽

Cerebrovascular Diseases ◽

Posterior Circulation ◽

Diagnostic Methods ◽

Healthy Controls ◽

Nucleotide Polymorphisms ◽

C Reactive Protein ◽

Single Nucleotide ◽

Reactive Protein

Abstract Background: Posterior circulation ischemia (PCI) and cerebral infarction (CI) are major cerebrovascular diseases. Screening potential genetic variations such as single nucleotide polymorphisms (SNPs) in genes involved in the immune system pathway is a reliable assessment of potential risk factors for PCI and CI. Moreover, C-reactive protein (CRP) is recognized as a sensitive biomarker for systemic inflammation and secondary lesions related to cerebrovascular diseases, which has a significant clinical significance.Methods: We collected whole blood and plasma samples from 39 healthy controls, 23 PCI and 18 CI subjects. Allele-specific polymerase chain reaction (AS-PCR) was used to detect mutations in SNPs. The plasma CRP levels were measured using the newly developed single molecule array (Simoa) method. Results: We detected mutations in three SNPs such as rs3093077, rs3917368 and rs4129267, which are associated with an increased risk of PCI and CI. We also captured a significant increase in Simoa-measured CRP levels in plasma of patients with PCI and CI, compared to healthy controls.Conclusions: These three identified SNPs can be used as a prediction and evaluation of risk for PCI and CI. Our results demonstrate the high sensitivity of Simoa in detecting plasma CRP levels. Our study highlights that AS-PCR and Simoa technology can be used as sensitive clinical diagnostic methods for PCI and CI.

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